Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04275 and PS417_04280 are separated by 48 nucleotides PS417_04280 and PS417_04285 are separated by 16 nucleotides PS417_04285 and PS417_04290 are separated by 45 nucleotides PS417_04290 and PS417_04295 are separated by 108 nucleotides
PS417_04275: PS417_04275 - hypothetical protein, at 956,859 to 960,674
_04275
PS417_04280: PS417_04280 - carbon-nitrogen hydrolase, at 960,723 to 961,571
_04280
PS417_04285: PS417_04285 - protease TldD, at 961,588 to 963,030
_04285
PS417_04290: PS417_04290 - hypothetical protein, at 963,076 to 963,597
_04290
PS417_04295: PS417_04295 - peptidase PmbA, at 963,706 to 965,052
_04295
Position (kb) 961
962
963
964 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.688 kb on - strand at 960.833 kb on - strand, within PS417_04280 at 960.953 kb on - strand, within PS417_04280 at 960.953 kb on - strand, within PS417_04280 at 961.129 kb on - strand, within PS417_04280 at 961.195 kb on - strand, within PS417_04280 at 961.195 kb on - strand, within PS417_04280 at 961.339 kb on - strand, within PS417_04280 at 961.339 kb on - strand, within PS417_04280 at 961.390 kb on - strand, within PS417_04280 at 961.575 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.584 kb on - strand at 961.584 kb on - strand at 961.584 kb on - strand at 961.587 kb on + strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.709 kb on - strand at 961.709 kb on - strand at 961.831 kb on + strand, within PS417_04285 at 961.951 kb on + strand, within PS417_04285 at 961.952 kb on - strand, within PS417_04285 at 962.228 kb on - strand, within PS417_04285 at 962.422 kb on + strand, within PS417_04285 at 962.575 kb on + strand, within PS417_04285 at 962.575 kb on + strand, within PS417_04285 at 962.576 kb on - strand, within PS417_04285 at 962.644 kb on + strand, within PS417_04285 at 962.680 kb on + strand, within PS417_04285 at 962.680 kb on + strand, within PS417_04285 at 962.680 kb on + strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.681 kb on - strand, within PS417_04285 at 962.740 kb on + strand, within PS417_04285 at 962.741 kb on - strand, within PS417_04285 at 962.813 kb on - strand, within PS417_04285 at 962.938 kb on - strand at 962.938 kb on - strand at 963.029 kb on - strand at 963.116 kb on + strand at 963.116 kb on + strand at 963.129 kb on + strand, within PS417_04290 at 963.154 kb on - strand, within PS417_04290 at 963.288 kb on + strand, within PS417_04290 at 963.402 kb on + strand, within PS417_04290 at 963.501 kb on + strand, within PS417_04290 at 963.502 kb on - strand, within PS417_04290 at 963.585 kb on - strand at 963.910 kb on + strand, within PS417_04295 at 963.910 kb on + strand, within PS417_04295 at 963.910 kb on + strand, within PS417_04295 at 963.910 kb on + strand, within PS417_04295 at 963.911 kb on - strand, within PS417_04295 at 963.911 kb on - strand, within PS417_04295
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 960,606 + +0.4 960,606 + -0.7 960,606 + +0.1 960,606 + -2.3 960,606 + -1.7 960,606 + -0.0 960,607 - -0.2 960,607 - +0.2 960,607 - +0.3 960,607 - +0.7 960,607 - -1.3 960,687 + +0.8 960,687 + +1.9 960,687 + +0.3 960,687 + +2.1 960,688 - +0.5 960,833 - PS417_04280 0.13 +0.8 960,953 - PS417_04280 0.27 -1.0 960,953 - PS417_04280 0.27 -0.5 961,129 - PS417_04280 0.48 -0.4 961,195 - PS417_04280 0.56 -1.5 961,195 - PS417_04280 0.56 -0.5 961,339 - PS417_04280 0.73 -0.9 961,339 - PS417_04280 0.73 -1.3 961,390 - PS417_04280 0.79 -0.3 961,575 + +0.7 961,583 + -1.0 961,583 + -0.9 961,583 + -0.1 961,583 + +0.6 961,583 + -0.3 961,584 - +1.6 961,584 - +0.7 961,584 - +0.6 961,587 + -0.4 961,588 - -2.9 961,588 - -0.8 961,588 - -0.9 961,588 - -0.6 961,588 - -0.2 961,588 - -1.0 961,709 - -1.5 961,709 - -1.2 961,831 + PS417_04285 0.17 -1.0 961,951 + PS417_04285 0.25 -0.5 961,952 - PS417_04285 0.25 -0.6 962,228 - PS417_04285 0.44 +0.0 962,422 + PS417_04285 0.58 -0.4 962,575 + PS417_04285 0.68 -2.9 962,575 + PS417_04285 0.68 +0.5 962,576 - PS417_04285 0.68 -2.9 962,644 + PS417_04285 0.73 -2.2 962,680 + PS417_04285 0.76 -0.7 962,680 + PS417_04285 0.76 -0.4 962,680 + PS417_04285 0.76 -0.3 962,681 - PS417_04285 0.76 -1.2 962,681 - PS417_04285 0.76 -0.5 962,681 - PS417_04285 0.76 -0.4 962,681 - PS417_04285 0.76 -1.9 962,681 - PS417_04285 0.76 -0.1 962,681 - PS417_04285 0.76 -1.4 962,740 + PS417_04285 0.80 +0.5 962,741 - PS417_04285 0.80 -0.3 962,813 - PS417_04285 0.85 -0.5 962,938 - +1.1 962,938 - +0.3 963,029 - -1.2 963,116 + -0.1 963,116 + -0.4 963,129 + PS417_04290 0.10 -1.2 963,154 - PS417_04290 0.15 -0.7 963,288 + PS417_04290 0.41 -0.4 963,402 + PS417_04290 0.62 -0.7 963,501 + PS417_04290 0.81 -0.4 963,502 - PS417_04290 0.82 -0.3 963,585 - -0.7 963,910 + PS417_04295 0.15 -1.3 963,910 + PS417_04295 0.15 -1.8 963,910 + PS417_04295 0.15 -3.2 963,910 + PS417_04295 0.15 -0.8 963,911 - PS417_04295 0.15 -0.0 963,911 - PS417_04295 0.15 -2.6
Or see this region's nucleotide sequence