Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23050 and PS417_23055 are separated by 238 nucleotides PS417_23055 and PS417_23060 are separated by 13 nucleotides PS417_23060 and PS417_23065 are separated by 167 nucleotides
PS417_23050: PS417_23050 - trehalose PTS system, EII-BC components TreB <small>(from data)</small>, at 4,996,268 to 4,997,710
_23050
PS417_23055: PS417_23055 - GntR family transcriptional regulator, at 4,997,949 to 4,998,653
_23055
PS417_23060: PS417_23060 - aminoglycoside phosphotransferase, at 4,998,667 to 4,999,437
_23060
PS417_23065: PS417_23065 - hypothetical protein, at 4,999,605 to 5,000,162
_23065
Position (kb) 4998
4999
5000 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4997.697 kb on + strand at 4997.698 kb on - strand at 4997.785 kb on + strand at 4997.959 kb on - strand at 4997.959 kb on - strand at 4997.971 kb on - strand at 4998.426 kb on + strand, within PS417_23055 at 4998.426 kb on + strand, within PS417_23055 at 4998.595 kb on - strand at 4998.620 kb on + strand at 4998.989 kb on + strand, within PS417_23060 at 4998.990 kb on - strand, within PS417_23060 at 4999.009 kb on - strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.327 kb on - strand, within PS417_23060 at 4999.327 kb on - strand, within PS417_23060 at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.376 kb on - strand at 4999.376 kb on - strand at 4999.411 kb on + strand at 4999.412 kb on - strand at 4999.672 kb on + strand, within PS417_23065 at 4999.790 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.863 kb on - strand, within PS417_23065 at 4999.878 kb on - strand, within PS417_23065 at 4999.901 kb on - strand at 4999.921 kb on + strand, within PS417_23065 at 4999.921 kb on + strand, within PS417_23065 at 4999.922 kb on - strand, within PS417_23065 at 4999.922 kb on - strand, within PS417_23065 at 4999.985 kb on - strand, within PS417_23065 at 4999.985 kb on - strand, within PS417_23065 at 5000.014 kb on - strand, within PS417_23065 at 5000.014 kb on - strand, within PS417_23065 at 5000.078 kb on + strand, within PS417_23065 at 5000.117 kb on + strand at 5000.227 kb on - strand at 5000.382 kb on + strand at 5000.383 kb on - strand at 5000.385 kb on - strand at 5000.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,997,697 + +0.3 4,997,698 - -0.2 4,997,785 + +0.3 4,997,959 - +2.1 4,997,959 - -0.3 4,997,971 - -0.1 4,998,426 + PS417_23055 0.68 +0.5 4,998,426 + PS417_23055 0.68 -1.5 4,998,595 - -0.1 4,998,620 + +0.0 4,998,989 + PS417_23060 0.42 -1.2 4,998,990 - PS417_23060 0.42 -0.5 4,999,009 - PS417_23060 0.44 -1.0 4,999,065 + PS417_23060 0.52 -0.7 4,999,065 + PS417_23060 0.52 -0.9 4,999,065 + PS417_23060 0.52 -0.5 4,999,327 - PS417_23060 0.86 -0.2 4,999,327 - PS417_23060 0.86 -0.0 4,999,375 + +2.0 4,999,375 + -0.0 4,999,375 + +0.5 4,999,375 + +1.0 4,999,375 + -0.4 4,999,375 + +0.9 4,999,375 + -0.4 4,999,376 - +0.8 4,999,376 - -0.1 4,999,411 + +0.4 4,999,412 - +0.5 4,999,672 + PS417_23065 0.12 +0.3 4,999,790 + PS417_23065 0.33 +0.5 4,999,862 + PS417_23065 0.46 -3.4 4,999,862 + PS417_23065 0.46 +0.5 4,999,862 + PS417_23065 0.46 +0.1 4,999,862 + PS417_23065 0.46 -0.2 4,999,863 - PS417_23065 0.46 -0.9 4,999,878 - PS417_23065 0.49 -1.4 4,999,901 - +0.4 4,999,921 + PS417_23065 0.57 +0.1 4,999,921 + PS417_23065 0.57 +0.4 4,999,922 - PS417_23065 0.57 +1.1 4,999,922 - PS417_23065 0.57 +0.2 4,999,985 - PS417_23065 0.68 +0.5 4,999,985 - PS417_23065 0.68 +1.1 5,000,014 - PS417_23065 0.73 +0.5 5,000,014 - PS417_23065 0.73 +0.0 5,000,078 + PS417_23065 0.85 -0.1 5,000,117 + +0.1 5,000,227 - -0.8 5,000,382 + -1.7 5,000,383 - -0.1 5,000,385 - -0.2 5,000,387 - -0.5
Or see this region's nucleotide sequence