Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22695 and PS417_22700 are separated by 196 nucleotides PS417_22700 and PS417_22705 are separated by 74 nucleotides
PS417_22695: PS417_22695 - glyceraldehyde-3-phosphate dehydrogenase, at 4,915,330 to 4,916,331
_22695
PS417_22700: PS417_22700 - RNA polymerase subunit sigma-24, at 4,916,528 to 4,917,046
_22700
PS417_22705: PS417_22705 - glycerol-3-phosphate ABC transporter substrate-binding protein, at 4,917,121 to 4,918,089
_22705
Position (kb)
4916
4917
4918 Strain fitness (log2 ratio)
-2
-1
0
1 at 4915.665 kb on + strand, within PS417_22695 at 4915.665 kb on + strand, within PS417_22695 at 4915.666 kb on - strand, within PS417_22695 at 4915.666 kb on - strand, within PS417_22695 at 4915.733 kb on - strand, within PS417_22695 at 4916.152 kb on + strand, within PS417_22695 at 4916.155 kb on - strand, within PS417_22695 at 4916.155 kb on - strand, within PS417_22695 at 4916.263 kb on + strand at 4916.293 kb on - strand at 4916.293 kb on - strand at 4916.301 kb on + strand at 4916.329 kb on + strand at 4916.330 kb on - strand at 4916.643 kb on - strand, within PS417_22700 at 4916.702 kb on + strand, within PS417_22700 at 4917.008 kb on - strand at 4917.016 kb on - strand at 4917.016 kb on - strand at 4917.016 kb on - strand at 4917.016 kb on - strand at 4917.016 kb on - strand at 4917.092 kb on + strand at 4917.092 kb on + strand at 4917.326 kb on + strand, within PS417_22705 at 4917.531 kb on - strand, within PS417_22705 at 4917.531 kb on - strand, within PS417_22705 at 4917.994 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,915,665 + PS417_22695 0.33 +0.2 4,915,665 + PS417_22695 0.33 +0.5 4,915,666 - PS417_22695 0.34 +0.6 4,915,666 - PS417_22695 0.34 -0.5 4,915,733 - PS417_22695 0.40 +0.3 4,916,152 + PS417_22695 0.82 +0.3 4,916,155 - PS417_22695 0.82 +1.0 4,916,155 - PS417_22695 0.82 -0.3 4,916,263 + -0.5 4,916,293 - +0.8 4,916,293 - -0.4 4,916,301 + -0.1 4,916,329 + +0.3 4,916,330 - -0.2 4,916,643 - PS417_22700 0.22 -0.1 4,916,702 + PS417_22700 0.34 -1.9 4,917,008 - +1.0 4,917,016 - +0.3 4,917,016 - +0.7 4,917,016 - -0.1 4,917,016 - -1.5 4,917,016 - +0.4 4,917,092 + +0.3 4,917,092 + -0.9 4,917,326 + PS417_22705 0.21 -1.7 4,917,531 - PS417_22705 0.42 +0.8 4,917,531 - PS417_22705 0.42 +0.2 4,917,994 + -0.2
Or see this region's nucleotide sequence