Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19735 and PS417_19740 are separated by 4 nucleotides PS417_19740 and PS417_19745 overlap by 1 nucleotides PS417_19745 and PS417_19750 are separated by 1 nucleotides PS417_19750 and PS417_19755 are separated by 46 nucleotides
PS417_19735: PS417_19735 - flagellar biosynthesis protein FliQ, at 4,288,250 to 4,288,519
_19735
PS417_19740: PS417_19740 - flagellar biosynthesis protein flip, at 4,288,524 to 4,289,267
_19740
PS417_19745: PS417_19745 - flagellar assembly protein FliO, at 4,289,267 to 4,289,710
_19745
PS417_19750: PS417_19750 - flagellar motor switch protein, at 4,289,712 to 4,290,179
_19750
PS417_19755: PS417_19755 - flagellar motor switch protein FliM, at 4,290,226 to 4,291,194
_19755
Position (kb)
4289
4290 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.294 kb on - strand, within PS417_19735 at 4288.525 kb on + strand at 4288.853 kb on + strand, within PS417_19740 at 4288.854 kb on - strand, within PS417_19740 at 4288.854 kb on - strand, within PS417_19740 at 4289.106 kb on - strand, within PS417_19740 at 4289.268 kb on + strand at 4289.268 kb on + strand at 4289.383 kb on + strand, within PS417_19745 at 4289.612 kb on + strand, within PS417_19745 at 4289.613 kb on - strand, within PS417_19745 at 4289.613 kb on - strand, within PS417_19745 at 4289.704 kb on - strand at 4289.704 kb on - strand at 4290.016 kb on + strand, within PS417_19750 at 4290.180 kb on + strand at 4290.180 kb on + strand at 4290.180 kb on + strand at 4290.181 kb on - strand at 4290.181 kb on - strand at 4290.181 kb on - strand at 4290.181 kb on - strand at 4290.213 kb on + strand at 4290.213 kb on + strand at 4290.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,288,293 + PS417_19735 0.16 -2.1 4,288,293 + PS417_19735 0.16 -3.0 4,288,293 + PS417_19735 0.16 -5.6 4,288,294 - PS417_19735 0.16 -3.7 4,288,525 + -4.9 4,288,853 + PS417_19740 0.44 -3.5 4,288,854 - PS417_19740 0.44 -1.7 4,288,854 - PS417_19740 0.44 -7.6 4,289,106 - PS417_19740 0.78 -3.8 4,289,268 + -0.6 4,289,268 + -1.4 4,289,383 + PS417_19745 0.26 -4.0 4,289,612 + PS417_19745 0.78 -6.0 4,289,613 - PS417_19745 0.78 -4.0 4,289,613 - PS417_19745 0.78 -2.5 4,289,704 - -1.9 4,289,704 - -4.5 4,290,016 + PS417_19750 0.65 -3.0 4,290,180 + -6.0 4,290,180 + -2.4 4,290,180 + -1.1 4,290,181 - -2.2 4,290,181 - -6.4 4,290,181 - -1.9 4,290,181 - -4.5 4,290,213 + -2.6 4,290,213 + -1.9 4,290,214 - -0.5
Or see this region's nucleotide sequence