Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07620 and PS417_07625 are separated by 49 nucleotides PS417_07625 and PS417_07630 are separated by 9 nucleotides PS417_07630 and PS417_07635 are separated by 11 nucleotides
PS417_07620: PS417_07620 - beta-hexosaminidase, at 1,676,293 to 1,677,303
_07620
PS417_07625: PS417_07625 - helicase, at 1,677,353 to 1,680,046
_07625
PS417_07630: PS417_07630 - hypothetical protein, at 1,680,056 to 1,680,622
_07630
PS417_07635: PS417_07635 - transcription-repair coupling factor, at 1,680,634 to 1,684,083
_07635
Position (kb)
1677
1678
1679
1680
1681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1676.579 kb on - strand, within PS417_07620 at 1676.938 kb on + strand, within PS417_07620 at 1677.447 kb on - strand at 1677.751 kb on - strand, within PS417_07625 at 1677.868 kb on + strand, within PS417_07625 at 1677.868 kb on + strand, within PS417_07625 at 1678.050 kb on + strand, within PS417_07625 at 1678.051 kb on - strand, within PS417_07625 at 1678.389 kb on + strand, within PS417_07625 at 1678.464 kb on + strand, within PS417_07625 at 1678.465 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.486 kb on - strand, within PS417_07625 at 1678.513 kb on + strand, within PS417_07625 at 1678.513 kb on + strand at 1678.514 kb on - strand, within PS417_07625 at 1678.527 kb on + strand, within PS417_07625 at 1678.527 kb on + strand, within PS417_07625 at 1678.528 kb on - strand, within PS417_07625 at 1678.605 kb on - strand, within PS417_07625 at 1678.950 kb on + strand, within PS417_07625 at 1678.950 kb on + strand, within PS417_07625 at 1678.993 kb on - strand, within PS417_07625 at 1679.168 kb on - strand, within PS417_07625 at 1679.168 kb on - strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.523 kb on + strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.524 kb on - strand, within PS417_07625 at 1679.535 kb on + strand, within PS417_07625 at 1679.535 kb on + strand, within PS417_07625 at 1679.569 kb on - strand, within PS417_07625 at 1679.990 kb on - strand at 1680.147 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.393 kb on + strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.394 kb on - strand, within PS417_07630 at 1680.424 kb on + strand, within PS417_07630 at 1680.427 kb on + strand, within PS417_07630 at 1680.428 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.452 kb on - strand, within PS417_07630 at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.678 kb on + strand at 1680.679 kb on - strand at 1680.682 kb on - strand at 1680.682 kb on - strand at 1680.682 kb on - strand at 1680.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,676,579 - PS417_07620 0.28 -2.5 1,676,938 + PS417_07620 0.64 -2.2 1,677,447 - +0.4 1,677,751 - PS417_07625 0.15 -0.7 1,677,868 + PS417_07625 0.19 +0.1 1,677,868 + PS417_07625 0.19 +0.2 1,678,050 + PS417_07625 0.26 +0.6 1,678,051 - PS417_07625 0.26 +0.2 1,678,389 + PS417_07625 0.38 -0.6 1,678,464 + PS417_07625 0.41 +0.9 1,678,465 - PS417_07625 0.41 +0.4 1,678,486 - PS417_07625 0.42 -0.5 1,678,486 - PS417_07625 0.42 -0.1 1,678,486 - PS417_07625 0.42 +0.2 1,678,513 + PS417_07625 0.43 -1.4 1,678,513 + +0.9 1,678,514 - PS417_07625 0.43 -0.0 1,678,527 + PS417_07625 0.44 -1.1 1,678,527 + PS417_07625 0.44 -1.2 1,678,528 - PS417_07625 0.44 +0.5 1,678,605 - PS417_07625 0.46 -0.6 1,678,950 + PS417_07625 0.59 -0.2 1,678,950 + PS417_07625 0.59 +1.2 1,678,993 - PS417_07625 0.61 -2.4 1,679,168 - PS417_07625 0.67 -1.3 1,679,168 - PS417_07625 0.67 +0.5 1,679,523 + PS417_07625 0.81 -0.3 1,679,523 + PS417_07625 0.81 +0.2 1,679,523 + PS417_07625 0.81 +0.7 1,679,524 - PS417_07625 0.81 +0.3 1,679,524 - PS417_07625 0.81 +0.1 1,679,524 - PS417_07625 0.81 +0.8 1,679,524 - PS417_07625 0.81 -0.0 1,679,524 - PS417_07625 0.81 -1.1 1,679,524 - PS417_07625 0.81 +0.3 1,679,524 - PS417_07625 0.81 +0.0 1,679,535 + PS417_07625 0.81 -0.2 1,679,535 + PS417_07625 0.81 +1.9 1,679,569 - PS417_07625 0.82 +0.4 1,679,990 - -0.2 1,680,147 + PS417_07630 0.16 -0.4 1,680,393 + PS417_07630 0.59 +1.8 1,680,393 + PS417_07630 0.59 +0.3 1,680,393 + PS417_07630 0.59 +0.1 1,680,394 - PS417_07630 0.60 -2.1 1,680,394 - PS417_07630 0.60 +0.4 1,680,394 - PS417_07630 0.60 +0.2 1,680,424 + PS417_07630 0.65 -0.9 1,680,427 + PS417_07630 0.65 -0.1 1,680,428 - PS417_07630 0.66 -1.5 1,680,452 - PS417_07630 0.70 +0.4 1,680,452 - PS417_07630 0.70 -1.2 1,680,452 - PS417_07630 0.70 +0.0 1,680,678 + -1.6 1,680,678 + -0.8 1,680,678 + -0.8 1,680,678 + -2.0 1,680,678 + -1.2 1,680,679 - -1.6 1,680,682 - -0.1 1,680,682 - -1.0 1,680,682 - -2.7 1,680,729 + -0.7
Or see this region's nucleotide sequence