Experiment: marine_broth_2216 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1928 and B158DRAFT_1929 are separated by 30 nucleotides B158DRAFT_1929 and B158DRAFT_1930 are separated by 34 nucleotides B158DRAFT_1930 and B158DRAFT_1931 are separated by 143 nucleotides B158DRAFT_1931 and B158DRAFT_1932 are separated by 3 nucleotides B158DRAFT_1932 and B158DRAFT_1933 are separated by 79 nucleotides
B158DRAFT_1928: B158DRAFT_1928 - Uncharacterized protein conserved in bacteria, at 64,243 to 65,277
_1928
B158DRAFT_1929: B158DRAFT_1929 - transcription elongation factor GreB, at 65,308 to 65,796
_1929
B158DRAFT_1930: B158DRAFT_1930 - hypothetical protein, at 65,831 to 66,043
_1930
B158DRAFT_1931: B158DRAFT_1931 - Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase., at 66,187 to 67,422
_1931
B158DRAFT_1932: B158DRAFT_1932 - Response regulator consisting of a CheY-like receiver domain and a Fis-type HTH domain, at 67,426 to 68,001
_1932
B158DRAFT_1933: B158DRAFT_1933 - Outer membrane receptor for Fe3+-dicitrate, at 68,081 to 70,303
_1933
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 65.256 kb on + strand at 65.279 kb on - strand at 65.294 kb on + strand at 65.322 kb on + strand at 65.345 kb on + strand at 65.345 kb on + strand at 65.345 kb on + strand at 65.345 kb on + strand at 65.353 kb on - strand at 65.353 kb on - strand at 65.373 kb on + strand, within B158DRAFT_1929 at 65.381 kb on - strand, within B158DRAFT_1929 at 65.427 kb on - strand, within B158DRAFT_1929 at 65.458 kb on - strand, within B158DRAFT_1929 at 65.474 kb on - strand, within B158DRAFT_1929 at 65.578 kb on + strand, within B158DRAFT_1929 at 65.837 kb on - strand at 66.060 kb on + strand at 66.068 kb on - strand at 66.183 kb on + strand at 66.183 kb on + strand at 66.190 kb on - strand at 66.191 kb on - strand at 66.191 kb on - strand at 66.191 kb on - strand at 66.202 kb on - strand at 66.281 kb on - strand at 66.291 kb on + strand at 66.306 kb on - strand at 66.358 kb on + strand, within B158DRAFT_1931 at 66.370 kb on - strand, within B158DRAFT_1931 at 66.376 kb on - strand, within B158DRAFT_1931 at 66.414 kb on + strand, within B158DRAFT_1931 at 66.511 kb on - strand, within B158DRAFT_1931 at 66.520 kb on + strand, within B158DRAFT_1931 at 66.528 kb on - strand, within B158DRAFT_1931 at 66.600 kb on - strand, within B158DRAFT_1931 at 66.607 kb on - strand, within B158DRAFT_1931 at 66.647 kb on - strand, within B158DRAFT_1931 at 66.649 kb on + strand, within B158DRAFT_1931 at 66.657 kb on - strand, within B158DRAFT_1931 at 66.662 kb on + strand, within B158DRAFT_1931 at 66.680 kb on - strand, within B158DRAFT_1931 at 66.683 kb on - strand, within B158DRAFT_1931 at 66.732 kb on + strand, within B158DRAFT_1931 at 66.732 kb on + strand, within B158DRAFT_1931 at 66.740 kb on - strand, within B158DRAFT_1931 at 66.740 kb on - strand, within B158DRAFT_1931 at 66.740 kb on - strand, within B158DRAFT_1931 at 66.754 kb on + strand, within B158DRAFT_1931 at 66.754 kb on + strand, within B158DRAFT_1931 at 66.825 kb on - strand, within B158DRAFT_1931 at 66.835 kb on + strand, within B158DRAFT_1931 at 66.878 kb on - strand, within B158DRAFT_1931 at 66.911 kb on + strand, within B158DRAFT_1931 at 66.920 kb on + strand, within B158DRAFT_1931 at 66.920 kb on + strand, within B158DRAFT_1931 at 66.920 kb on + strand, within B158DRAFT_1931 at 66.921 kb on + strand, within B158DRAFT_1931 at 66.944 kb on - strand, within B158DRAFT_1931 at 67.006 kb on - strand, within B158DRAFT_1931 at 67.043 kb on + strand, within B158DRAFT_1931 at 67.045 kb on - strand, within B158DRAFT_1931 at 67.228 kb on + strand, within B158DRAFT_1931 at 67.236 kb on - strand, within B158DRAFT_1931 at 67.236 kb on - strand, within B158DRAFT_1931 at 67.284 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.348 kb on + strand at 67.387 kb on + strand at 67.440 kb on + strand at 67.452 kb on + strand at 67.457 kb on + strand at 67.470 kb on - strand at 67.526 kb on + strand, within B158DRAFT_1932 at 67.526 kb on + strand, within B158DRAFT_1932 at 67.526 kb on + strand, within B158DRAFT_1932 at 67.534 kb on - strand, within B158DRAFT_1932 at 67.534 kb on - strand, within B158DRAFT_1932 at 67.536 kb on + strand, within B158DRAFT_1932 at 67.544 kb on + strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.606 kb on + strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.625 kb on + strand, within B158DRAFT_1932 at 67.625 kb on + strand, within B158DRAFT_1932 at 67.633 kb on - strand, within B158DRAFT_1932 at 67.635 kb on + strand, within B158DRAFT_1932 at 67.640 kb on + strand, within B158DRAFT_1932 at 67.643 kb on - strand, within B158DRAFT_1932 at 67.688 kb on + strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.786 kb on + strand, within B158DRAFT_1932 at 67.826 kb on + strand, within B158DRAFT_1932 at 67.870 kb on + strand, within B158DRAFT_1932 at 67.914 kb on + strand, within B158DRAFT_1932 at 67.953 kb on + strand at 67.953 kb on - strand at 67.953 kb on - strand at 67.958 kb on + strand at 67.958 kb on + strand at 68.066 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.132 kb on - strand at 68.132 kb on - strand at 68.155 kb on + strand at 68.182 kb on + strand at 68.209 kb on + strand at 68.212 kb on + strand at 68.217 kb on - strand at 68.219 kb on + strand at 68.219 kb on + strand at 68.219 kb on + strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.235 kb on + strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.238 kb on + strand at 68.272 kb on + strand at 68.293 kb on - strand at 68.300 kb on + strand at 68.308 kb on - strand, within B158DRAFT_1933 at 68.310 kb on + strand, within B158DRAFT_1933 at 68.318 kb on - strand, within B158DRAFT_1933 at 68.323 kb on - strand, within B158DRAFT_1933 at 68.340 kb on + strand, within B158DRAFT_1933 at 68.348 kb on - strand, within B158DRAFT_1933 at 68.348 kb on - strand, within B158DRAFT_1933 at 68.373 kb on + strand, within B158DRAFT_1933 at 68.386 kb on - strand, within B158DRAFT_1933 at 68.386 kb on - strand, within B158DRAFT_1933
Per-strain Table
Position Strand Gene LocusTag Fraction marine_broth_2216 with nitrite 20 mM remove 65,256 + +0.5 65,279 - +0.1 65,294 + -0.4 65,322 + +0.0 65,345 + +0.1 65,345 + +0.1 65,345 + -0.2 65,345 + +0.8 65,353 - -0.0 65,353 - +0.1 65,373 + B158DRAFT_1929 0.13 -0.4 65,381 - B158DRAFT_1929 0.15 -0.2 65,427 - B158DRAFT_1929 0.24 +0.2 65,458 - B158DRAFT_1929 0.31 -0.4 65,474 - B158DRAFT_1929 0.34 -0.9 65,578 + B158DRAFT_1929 0.55 -0.2 65,837 - +0.0 66,060 + -0.1 66,068 - -0.8 66,183 + +0.8 66,183 + -1.3 66,190 - -0.6 66,191 - -0.2 66,191 - -2.3 66,191 - +0.0 66,202 - -1.6 66,281 - +0.0 66,291 + +2.0 66,306 - +0.0 66,358 + B158DRAFT_1931 0.14 +1.0 66,370 - B158DRAFT_1931 0.15 -1.7 66,376 - B158DRAFT_1931 0.15 -0.6 66,414 + B158DRAFT_1931 0.18 +0.0 66,511 - B158DRAFT_1931 0.26 -1.8 66,520 + B158DRAFT_1931 0.27 +0.0 66,528 - B158DRAFT_1931 0.28 -0.6 66,600 - B158DRAFT_1931 0.33 -1.0 66,607 - B158DRAFT_1931 0.34 -0.3 66,647 - B158DRAFT_1931 0.37 -2.4 66,649 + B158DRAFT_1931 0.37 -2.0 66,657 - B158DRAFT_1931 0.38 +0.0 66,662 + B158DRAFT_1931 0.38 -2.2 66,680 - B158DRAFT_1931 0.40 -1.0 66,683 - B158DRAFT_1931 0.40 -0.3 66,732 + B158DRAFT_1931 0.44 -0.6 66,732 + B158DRAFT_1931 0.44 -2.2 66,740 - B158DRAFT_1931 0.45 -1.6 66,740 - B158DRAFT_1931 0.45 -0.6 66,740 - B158DRAFT_1931 0.45 -1.0 66,754 + B158DRAFT_1931 0.46 -1.3 66,754 + B158DRAFT_1931 0.46 +1.0 66,825 - B158DRAFT_1931 0.52 -1.3 66,835 + B158DRAFT_1931 0.52 -0.6 66,878 - B158DRAFT_1931 0.56 -1.3 66,911 + B158DRAFT_1931 0.59 -2.6 66,920 + B158DRAFT_1931 0.59 -1.0 66,920 + B158DRAFT_1931 0.59 +0.0 66,920 + B158DRAFT_1931 0.59 -0.4 66,921 + B158DRAFT_1931 0.59 -1.0 66,944 - B158DRAFT_1931 0.61 +0.0 67,006 - B158DRAFT_1931 0.66 +1.0 67,043 + B158DRAFT_1931 0.69 -1.3 67,045 - B158DRAFT_1931 0.69 +0.7 67,228 + B158DRAFT_1931 0.84 -0.9 67,236 - B158DRAFT_1931 0.85 -3.4 67,236 - B158DRAFT_1931 0.85 -1.6 67,284 + B158DRAFT_1931 0.89 -3.5 67,285 + B158DRAFT_1931 0.89 +0.0 67,285 + B158DRAFT_1931 0.89 +0.0 67,285 + B158DRAFT_1931 0.89 +0.6 67,348 + +0.0 67,387 + -0.6 67,440 + +1.0 67,452 + -2.6 67,457 + +0.4 67,470 - -1.2 67,526 + B158DRAFT_1932 0.17 +2.0 67,526 + B158DRAFT_1932 0.17 +1.0 67,526 + B158DRAFT_1932 0.17 +0.0 67,534 - B158DRAFT_1932 0.19 -0.6 67,534 - B158DRAFT_1932 0.19 +0.0 67,536 + B158DRAFT_1932 0.19 -0.6 67,544 + B158DRAFT_1932 0.20 -2.2 67,544 - B158DRAFT_1932 0.20 -1.4 67,544 - B158DRAFT_1932 0.20 +0.0 67,544 - B158DRAFT_1932 0.20 +1.0 67,606 + B158DRAFT_1932 0.31 +1.0 67,614 - B158DRAFT_1932 0.33 +1.0 67,614 - B158DRAFT_1932 0.33 -1.0 67,614 - B158DRAFT_1932 0.33 -0.6 67,625 + B158DRAFT_1932 0.35 -0.3 67,625 + B158DRAFT_1932 0.35 -1.2 67,633 - B158DRAFT_1932 0.36 -1.3 67,635 + B158DRAFT_1932 0.36 -0.6 67,640 + B158DRAFT_1932 0.37 -0.3 67,643 - B158DRAFT_1932 0.38 -2.0 67,688 + B158DRAFT_1932 0.45 -2.0 67,696 - B158DRAFT_1932 0.47 -1.6 67,696 - B158DRAFT_1932 0.47 -2.3 67,696 - B158DRAFT_1932 0.47 -0.6 67,786 + B158DRAFT_1932 0.62 +1.0 67,826 + B158DRAFT_1932 0.69 -2.4 67,870 + B158DRAFT_1932 0.77 -1.0 67,914 + B158DRAFT_1932 0.85 -0.6 67,953 + -1.6 67,953 - -0.8 67,953 - +0.0 67,958 + +0.0 67,958 + -1.0 68,066 + -0.8 68,071 + +0.1 68,071 + +0.2 68,071 + -0.6 68,071 + -0.3 68,071 + -0.6 68,071 + -0.4 68,071 + -0.4 68,071 + -0.1 68,079 - -0.1 68,079 - -0.1 68,079 - -0.5 68,079 - +0.3 68,079 - +0.4 68,079 - -0.1 68,079 - +0.0 68,079 - -0.4 68,079 - -0.1 68,132 - -1.4 68,132 - +0.3 68,155 + +0.6 68,182 + +0.6 68,209 + -0.0 68,212 + -0.4 68,217 - +0.5 68,219 + +0.3 68,219 + -0.3 68,219 + +0.1 68,227 - -0.3 68,227 - +0.3 68,227 - +0.0 68,227 - -0.3 68,227 - +0.2 68,227 - -0.3 68,227 - -1.4 68,227 - -0.3 68,227 - -0.2 68,227 - +0.2 68,228 + -0.0 68,228 + -1.0 68,228 + -0.8 68,228 + -0.4 68,228 + -0.3 68,228 + -0.1 68,228 + -0.0 68,235 + -0.9 68,236 - -0.8 68,236 - -0.2 68,236 - -1.5 68,236 - -0.5 68,236 - +0.2 68,236 - +0.0 68,238 + -0.1 68,272 + -0.0 68,293 - -0.7 68,300 + -0.3 68,308 - B158DRAFT_1933 0.10 +0.2 68,310 + B158DRAFT_1933 0.10 -1.0 68,318 - B158DRAFT_1933 0.11 +1.2 68,323 - B158DRAFT_1933 0.11 -0.2 68,340 + B158DRAFT_1933 0.12 -1.6 68,348 - B158DRAFT_1933 0.12 +0.0 68,348 - B158DRAFT_1933 0.12 +0.5 68,373 + B158DRAFT_1933 0.13 +0.3 68,386 - B158DRAFT_1933 0.14 +0.7 68,386 - B158DRAFT_1933 0.14 -2.9
Or see this region's nucleotide sequence