Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS25085 and ABID97_RS25090 overlap by 4 nucleotides ABID97_RS25090 and ABID97_RS25095 overlap by 4 nucleotides ABID97_RS25095 and ABID97_RS25100 are separated by 31 nucleotides ABID97_RS25100 and ABID97_RS25105 are separated by 116 nucleotides
ABID97_RS25085: ABID97_RS25085 - 2-hydroxychromene-2-carboxylate isomerase, at 5,197,691 to 5,198,338
_RS25085
ABID97_RS25090: ABID97_RS25090 - DUF1289 domain-containing protein, at 5,198,335 to 5,198,604
_RS25090
ABID97_RS25095: ABID97_RS25095 - YbaK/EbsC family protein, at 5,198,601 to 5,199,089
_RS25095
ABID97_RS25100: ABID97_RS25100 - hydroxymethylglutaryl-CoA lyase, at 5,199,121 to 5,200,041
_RS25100
ABID97_RS25105: ABID97_RS25105 - glyoxylate/hydroxypyruvate reductase A, at 5,200,158 to 5,201,078
_RS25105
Position (kb)
5199
5200
5201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5198.235 kb on + strand, within ABID97_RS25085 at 5198.247 kb on + strand, within ABID97_RS25085 at 5198.247 kb on + strand, within ABID97_RS25085 at 5198.248 kb on - strand, within ABID97_RS25085 at 5198.320 kb on - strand at 5198.398 kb on + strand, within ABID97_RS25090 at 5199.194 kb on + strand at 5199.195 kb on - strand at 5199.260 kb on + strand, within ABID97_RS25100 at 5199.260 kb on + strand, within ABID97_RS25100 at 5199.260 kb on + strand, within ABID97_RS25100 at 5199.260 kb on + strand, within ABID97_RS25100 at 5199.260 kb on + strand, within ABID97_RS25100 at 5199.261 kb on - strand, within ABID97_RS25100 at 5199.261 kb on - strand, within ABID97_RS25100 at 5199.261 kb on - strand, within ABID97_RS25100 at 5199.407 kb on + strand, within ABID97_RS25100 at 5199.408 kb on - strand, within ABID97_RS25100 at 5199.408 kb on - strand, within ABID97_RS25100 at 5200.058 kb on + strand at 5200.058 kb on + strand at 5200.058 kb on + strand at 5200.058 kb on + strand at 5200.059 kb on - strand at 5200.059 kb on - strand at 5200.059 kb on - strand at 5200.059 kb on - strand at 5200.059 kb on - strand at 5200.332 kb on + strand, within ABID97_RS25105 at 5200.332 kb on + strand, within ABID97_RS25105 at 5200.333 kb on - strand, within ABID97_RS25105 at 5200.333 kb on - strand, within ABID97_RS25105 at 5200.333 kb on - strand, within ABID97_RS25105 at 5200.436 kb on - strand, within ABID97_RS25105 at 5200.714 kb on + strand, within ABID97_RS25105 at 5200.715 kb on - strand, within ABID97_RS25105 at 5200.715 kb on - strand, within ABID97_RS25105 at 5200.747 kb on + strand, within ABID97_RS25105 at 5200.792 kb on + strand, within ABID97_RS25105 at 5200.793 kb on - strand, within ABID97_RS25105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 5,198,235 + ABID97_RS25085 0.84 -0.5 5,198,247 + ABID97_RS25085 0.86 -1.5 5,198,247 + ABID97_RS25085 0.86 -1.1 5,198,248 - ABID97_RS25085 0.86 +0.5 5,198,320 - -0.2 5,198,398 + ABID97_RS25090 0.23 -0.1 5,199,194 + -1.3 5,199,195 - -1.0 5,199,260 + ABID97_RS25100 0.15 +2.0 5,199,260 + ABID97_RS25100 0.15 -0.2 5,199,260 + ABID97_RS25100 0.15 -2.5 5,199,260 + ABID97_RS25100 0.15 -1.4 5,199,260 + ABID97_RS25100 0.15 +2.0 5,199,261 - ABID97_RS25100 0.15 -0.7 5,199,261 - ABID97_RS25100 0.15 -2.6 5,199,261 - ABID97_RS25100 0.15 -1.4 5,199,407 + ABID97_RS25100 0.31 -0.5 5,199,408 - ABID97_RS25100 0.31 -2.9 5,199,408 - ABID97_RS25100 0.31 -1.4 5,200,058 + -0.7 5,200,058 + +0.0 5,200,058 + -0.3 5,200,058 + -0.4 5,200,059 - -0.3 5,200,059 - -0.0 5,200,059 - -0.5 5,200,059 - -1.9 5,200,059 - +0.5 5,200,332 + ABID97_RS25105 0.19 +2.0 5,200,332 + ABID97_RS25105 0.19 -1.0 5,200,333 - ABID97_RS25105 0.19 -1.9 5,200,333 - ABID97_RS25105 0.19 +0.4 5,200,333 - ABID97_RS25105 0.19 -0.8 5,200,436 - ABID97_RS25105 0.30 +0.6 5,200,714 + ABID97_RS25105 0.60 -0.0 5,200,715 - ABID97_RS25105 0.60 +0.3 5,200,715 - ABID97_RS25105 0.60 -1.4 5,200,747 + ABID97_RS25105 0.64 -0.5 5,200,792 + ABID97_RS25105 0.69 +0.7 5,200,793 - ABID97_RS25105 0.69 -0.2
Or see this region's nucleotide sequence