Strain Fitness in Variovorax sp. OAS795 around ABID97_RS25100

Experiment: R2A outgrowth control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABID97_RS25085 and ABID97_RS25090 overlap by 4 nucleotidesABID97_RS25090 and ABID97_RS25095 overlap by 4 nucleotidesABID97_RS25095 and ABID97_RS25100 are separated by 31 nucleotidesABID97_RS25100 and ABID97_RS25105 are separated by 116 nucleotides ABID97_RS25085: ABID97_RS25085 - 2-hydroxychromene-2-carboxylate isomerase, at 5,197,691 to 5,198,338 _RS25085 ABID97_RS25090: ABID97_RS25090 - DUF1289 domain-containing protein, at 5,198,335 to 5,198,604 _RS25090 ABID97_RS25095: ABID97_RS25095 - YbaK/EbsC family protein, at 5,198,601 to 5,199,089 _RS25095 ABID97_RS25100: ABID97_RS25100 - hydroxymethylglutaryl-CoA lyase, at 5,199,121 to 5,200,041 _RS25100 ABID97_RS25105: ABID97_RS25105 - glyoxylate/hydroxypyruvate reductase A, at 5,200,158 to 5,201,078 _RS25105 Position (kb) 5199 5200 5201Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5198.235 kb on + strand, within ABID97_RS25085at 5198.247 kb on + strand, within ABID97_RS25085at 5198.247 kb on + strand, within ABID97_RS25085at 5198.248 kb on - strand, within ABID97_RS25085at 5198.320 kb on - strandat 5198.398 kb on + strand, within ABID97_RS25090at 5199.194 kb on + strandat 5199.195 kb on - strandat 5199.260 kb on + strand, within ABID97_RS25100at 5199.260 kb on + strand, within ABID97_RS25100at 5199.260 kb on + strand, within ABID97_RS25100at 5199.260 kb on + strand, within ABID97_RS25100at 5199.260 kb on + strand, within ABID97_RS25100at 5199.261 kb on - strand, within ABID97_RS25100at 5199.261 kb on - strand, within ABID97_RS25100at 5199.261 kb on - strand, within ABID97_RS25100at 5199.407 kb on + strand, within ABID97_RS25100at 5199.408 kb on - strand, within ABID97_RS25100at 5199.408 kb on - strand, within ABID97_RS25100at 5200.058 kb on + strandat 5200.058 kb on + strandat 5200.058 kb on + strandat 5200.058 kb on + strandat 5200.059 kb on - strandat 5200.059 kb on - strandat 5200.059 kb on - strandat 5200.059 kb on - strandat 5200.059 kb on - strandat 5200.332 kb on + strand, within ABID97_RS25105at 5200.332 kb on + strand, within ABID97_RS25105at 5200.333 kb on - strand, within ABID97_RS25105at 5200.333 kb on - strand, within ABID97_RS25105at 5200.333 kb on - strand, within ABID97_RS25105at 5200.436 kb on - strand, within ABID97_RS25105at 5200.714 kb on + strand, within ABID97_RS25105at 5200.715 kb on - strand, within ABID97_RS25105at 5200.715 kb on - strand, within ABID97_RS25105at 5200.747 kb on + strand, within ABID97_RS25105at 5200.792 kb on + strand, within ABID97_RS25105at 5200.793 kb on - strand, within ABID97_RS25105

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A outgrowth control
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5,198,235 + ABID97_RS25085 0.84 -0.5
5,198,247 + ABID97_RS25085 0.86 -1.5
5,198,247 + ABID97_RS25085 0.86 -1.1
5,198,248 - ABID97_RS25085 0.86 +0.5
5,198,320 - -0.2
5,198,398 + ABID97_RS25090 0.23 -0.1
5,199,194 + -1.3
5,199,195 - -1.0
5,199,260 + ABID97_RS25100 0.15 +2.0
5,199,260 + ABID97_RS25100 0.15 -0.2
5,199,260 + ABID97_RS25100 0.15 -2.5
5,199,260 + ABID97_RS25100 0.15 -1.4
5,199,260 + ABID97_RS25100 0.15 +2.0
5,199,261 - ABID97_RS25100 0.15 -0.7
5,199,261 - ABID97_RS25100 0.15 -2.6
5,199,261 - ABID97_RS25100 0.15 -1.4
5,199,407 + ABID97_RS25100 0.31 -0.5
5,199,408 - ABID97_RS25100 0.31 -2.9
5,199,408 - ABID97_RS25100 0.31 -1.4
5,200,058 + -0.7
5,200,058 + +0.0
5,200,058 + -0.3
5,200,058 + -0.4
5,200,059 - -0.3
5,200,059 - -0.0
5,200,059 - -0.5
5,200,059 - -1.9
5,200,059 - +0.5
5,200,332 + ABID97_RS25105 0.19 +2.0
5,200,332 + ABID97_RS25105 0.19 -1.0
5,200,333 - ABID97_RS25105 0.19 -1.9
5,200,333 - ABID97_RS25105 0.19 +0.4
5,200,333 - ABID97_RS25105 0.19 -0.8
5,200,436 - ABID97_RS25105 0.30 +0.6
5,200,714 + ABID97_RS25105 0.60 -0.0
5,200,715 - ABID97_RS25105 0.60 +0.3
5,200,715 - ABID97_RS25105 0.60 -1.4
5,200,747 + ABID97_RS25105 0.64 -0.5
5,200,792 + ABID97_RS25105 0.69 +0.7
5,200,793 - ABID97_RS25105 0.69 -0.2

Or see this region's nucleotide sequence