Experiment: R2A with cyanide 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3594 and Atu3595 are separated by 182 nucleotides Atu3595 and Atu3596 are separated by 25 nucleotides Atu3596 and Atu3597 are separated by 114 nucleotides
Atu3594: Atu3594 - hypothetical protein, at 648,719 to 649,492
Atu3594
Atu3595: Atu3595 - electron transfer flavoprotein subunit beta, at 649,675 to 650,421
Atu3595
Atu3596: Atu3596 - electron transfer flavoprotein alpha subunit, at 650,447 to 651,376
Atu3596
Atu3597: Atu3597 - 3-hydroxybutyryl-CoA dehydrogenase, at 651,491 to 652,372
Atu3597
Position (kb) 650
651
652 Strain fitness (log2 ratio) -2
-1
0
1 at 649.480 kb on - strand at 649.491 kb on - strand at 649.491 kb on - strand at 649.573 kb on + strand at 650.415 kb on + strand at 651.352 kb on + strand at 651.530 kb on + strand at 651.589 kb on - strand, within Atu3597 at 651.663 kb on + strand, within Atu3597 at 651.696 kb on + strand, within Atu3597 at 651.753 kb on - strand, within Atu3597 at 651.805 kb on + strand, within Atu3597 at 651.805 kb on + strand, within Atu3597 at 651.813 kb on - strand, within Atu3597 at 651.901 kb on + strand, within Atu3597 at 652.069 kb on - strand, within Atu3597 at 652.108 kb on + strand, within Atu3597 at 652.108 kb on + strand, within Atu3597 at 652.108 kb on + strand, within Atu3597 at 652.116 kb on - strand, within Atu3597 at 652.116 kb on - strand, within Atu3597 at 652.272 kb on + strand, within Atu3597 at 652.272 kb on + strand, within Atu3597
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with cyanide 2.5 mM remove 649,480 - +0.3 649,491 - -0.3 649,491 - +0.2 649,573 + -0.0 650,415 + -1.1 651,352 + -0.3 651,530 + -0.3 651,589 - Atu3597 0.11 -0.2 651,663 + Atu3597 0.20 -0.7 651,696 + Atu3597 0.23 -1.3 651,753 - Atu3597 0.30 -1.3 651,805 + Atu3597 0.36 +0.1 651,805 + Atu3597 0.36 +0.1 651,813 - Atu3597 0.37 -0.6 651,901 + Atu3597 0.46 -1.8 652,069 - Atu3597 0.66 -1.5 652,108 + Atu3597 0.70 -0.6 652,108 + Atu3597 0.70 -1.2 652,108 + Atu3597 0.70 -1.8 652,116 - Atu3597 0.71 -2.5 652,116 - Atu3597 0.71 -0.0 652,272 + Atu3597 0.89 -0.8 652,272 + Atu3597 0.89 -0.3
Or see this region's nucleotide sequence