Experiment: R2A with cyanide 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2070 and Atu2071 are separated by 244 nucleotides Atu2071 and Atu2072 are separated by 141 nucleotides Atu2072 and Atu2073 are separated by 79 nucleotides Atu2073 and Atu2074 are separated by 141 nucleotides
Atu2070: Atu2070 - aminopeptidase P, at 2,030,680 to 2,032,521
Atu2070
Atu2071: Atu2071 - hypothetical protein, at 2,032,766 to 2,032,993
Atu2071
Atu2072: Atu2072 - ribosomal protein L11 methyltransferase, at 2,033,135 to 2,034,013
Atu2072
Atu2073: Atu2073 - membrane protein, at 2,034,093 to 2,034,701
Atu2073
Atu2074: Atu2074 - hypothetical protein, at 2,034,843 to 2,035,157
Atu2074
Position (kb)
2033
2034
2035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2032.178 kb on - strand, within Atu2070 at 2032.227 kb on + strand, within Atu2070 at 2032.230 kb on - strand, within Atu2070 at 2032.245 kb on - strand, within Atu2070 at 2032.270 kb on - strand, within Atu2070 at 2032.349 kb on - strand at 2032.519 kb on - strand at 2032.587 kb on + strand at 2032.590 kb on - strand at 2032.592 kb on + strand at 2032.622 kb on + strand at 2032.622 kb on + strand at 2032.710 kb on + strand at 2032.764 kb on + strand at 2032.764 kb on + strand at 2032.943 kb on - strand, within Atu2071 at 2033.030 kb on + strand at 2033.035 kb on + strand at 2033.035 kb on + strand at 2033.140 kb on - strand at 2033.141 kb on - strand at 2033.155 kb on + strand at 2033.167 kb on + strand at 2033.353 kb on + strand, within Atu2072 at 2033.353 kb on + strand, within Atu2072 at 2033.368 kb on + strand, within Atu2072 at 2033.490 kb on - strand, within Atu2072 at 2033.516 kb on + strand, within Atu2072 at 2033.669 kb on + strand, within Atu2072 at 2033.671 kb on + strand, within Atu2072 at 2033.671 kb on + strand, within Atu2072 at 2033.812 kb on - strand, within Atu2072 at 2033.867 kb on + strand, within Atu2072 at 2034.030 kb on + strand at 2034.030 kb on + strand at 2034.035 kb on + strand at 2034.038 kb on - strand at 2034.038 kb on - strand at 2034.038 kb on - strand at 2034.381 kb on - strand, within Atu2073 at 2034.408 kb on + strand, within Atu2073 at 2034.443 kb on + strand, within Atu2073 at 2034.482 kb on + strand, within Atu2073 at 2034.502 kb on + strand, within Atu2073 at 2034.515 kb on - strand, within Atu2073 at 2034.528 kb on - strand, within Atu2073 at 2034.615 kb on + strand, within Atu2073 at 2034.703 kb on + strand at 2034.716 kb on - strand at 2034.743 kb on + strand at 2034.787 kb on + strand at 2034.835 kb on - strand at 2034.864 kb on + strand at 2034.867 kb on - strand at 2034.867 kb on - strand at 2034.924 kb on - strand, within Atu2074 at 2035.008 kb on + strand, within Atu2074
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with cyanide 2.5 mM remove 2,032,178 - Atu2070 0.81 -0.7 2,032,227 + Atu2070 0.84 -0.4 2,032,230 - Atu2070 0.84 -0.3 2,032,245 - Atu2070 0.85 -2.1 2,032,270 - Atu2070 0.86 +0.6 2,032,349 - -0.7 2,032,519 - -0.9 2,032,587 + +0.2 2,032,590 - +0.7 2,032,592 + -0.7 2,032,622 + +0.1 2,032,622 + -0.7 2,032,710 + -0.1 2,032,764 + +0.2 2,032,764 + +0.1 2,032,943 - Atu2071 0.78 +1.2 2,033,030 + +0.4 2,033,035 + -0.7 2,033,035 + +0.3 2,033,140 - +1.7 2,033,141 - +0.5 2,033,155 + +0.7 2,033,167 + -0.1 2,033,353 + Atu2072 0.25 +0.2 2,033,353 + Atu2072 0.25 +0.6 2,033,368 + Atu2072 0.27 -2.6 2,033,490 - Atu2072 0.40 -0.4 2,033,516 + Atu2072 0.43 +0.6 2,033,669 + Atu2072 0.61 -0.1 2,033,671 + Atu2072 0.61 +1.1 2,033,671 + Atu2072 0.61 -0.2 2,033,812 - Atu2072 0.77 -0.1 2,033,867 + Atu2072 0.83 -0.3 2,034,030 + +0.5 2,034,030 + +0.7 2,034,035 + +2.1 2,034,038 - +0.1 2,034,038 - +0.5 2,034,038 - -0.3 2,034,381 - Atu2073 0.47 -2.0 2,034,408 + Atu2073 0.52 -2.3 2,034,443 + Atu2073 0.57 -1.4 2,034,482 + Atu2073 0.64 -2.3 2,034,502 + Atu2073 0.67 -2.5 2,034,515 - Atu2073 0.69 -2.5 2,034,528 - Atu2073 0.71 -1.1 2,034,615 + Atu2073 0.86 -1.7 2,034,703 + +0.7 2,034,716 - +0.4 2,034,743 + -0.1 2,034,787 + -0.4 2,034,835 - +0.7 2,034,864 + -0.2 2,034,867 - -0.0 2,034,867 - -0.1 2,034,924 - Atu2074 0.26 +0.2 2,035,008 + Atu2074 0.52 +0.1
Or see this region's nucleotide sequence