Experiment: R2A with cyanide 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0049 and Atu0050 are separated by 60 nucleotides Atu0050 and Atu0051 are separated by 73 nucleotides Atu0051 and Atu0052 are separated by 29 nucleotides
Atu0049: Atu0049 - hypothetical protein, at 51,048 to 51,533
Atu0049
Atu0050: Atu0050 - two component response regulator, at 51,594 to 52,181
Atu0050
Atu0051: Atu0051 - two component sensor kinase, at 52,255 to 53,562
Atu0051
Atu0052: Atu0052 - helicase, at 53,592 to 56,078
Atu0052
Position (kb) 52
53
54 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 51.388 kb on + strand, within Atu0049 at 51.396 kb on - strand, within Atu0049 at 51.560 kb on + strand at 51.568 kb on - strand at 51.696 kb on - strand, within Atu0050 at 51.771 kb on - strand, within Atu0050 at 51.771 kb on - strand, within Atu0050 at 51.783 kb on + strand, within Atu0050 at 51.817 kb on + strand, within Atu0050 at 51.817 kb on + strand, within Atu0050 at 51.822 kb on + strand, within Atu0050 at 51.890 kb on + strand, within Atu0050 at 51.911 kb on + strand, within Atu0050 at 51.937 kb on - strand, within Atu0050 at 51.939 kb on + strand, within Atu0050 at 51.988 kb on + strand, within Atu0050 at 51.996 kb on - strand, within Atu0050 at 52.006 kb on - strand, within Atu0050 at 52.169 kb on - strand at 52.262 kb on - strand at 52.297 kb on - strand at 52.306 kb on - strand at 52.343 kb on - strand at 52.396 kb on + strand, within Atu0051 at 52.441 kb on + strand, within Atu0051 at 52.464 kb on + strand, within Atu0051 at 52.472 kb on - strand, within Atu0051 at 52.472 kb on - strand, within Atu0051 at 52.491 kb on + strand, within Atu0051 at 52.510 kb on + strand, within Atu0051 at 52.653 kb on + strand, within Atu0051 at 52.796 kb on + strand, within Atu0051 at 52.913 kb on + strand, within Atu0051 at 53.157 kb on + strand, within Atu0051 at 53.227 kb on + strand, within Atu0051 at 53.235 kb on - strand, within Atu0051 at 53.272 kb on - strand, within Atu0051 at 53.292 kb on + strand, within Atu0051 at 53.300 kb on - strand, within Atu0051 at 53.300 kb on - strand, within Atu0051 at 53.377 kb on + strand, within Atu0051 at 53.420 kb on + strand, within Atu0051 at 53.505 kb on + strand at 53.505 kb on + strand at 53.505 kb on + strand at 53.513 kb on - strand at 53.513 kb on - strand at 53.513 kb on - strand at 53.571 kb on + strand at 53.572 kb on - strand at 53.579 kb on - strand at 53.579 kb on - strand at 53.579 kb on - strand at 53.606 kb on + strand at 53.614 kb on - strand at 53.693 kb on + strand at 53.738 kb on + strand at 53.738 kb on + strand at 53.738 kb on + strand at 53.746 kb on - strand at 53.750 kb on + strand at 53.760 kb on + strand at 53.760 kb on + strand at 53.875 kb on + strand, within Atu0052 at 53.919 kb on - strand, within Atu0052 at 53.930 kb on - strand, within Atu0052 at 53.991 kb on + strand, within Atu0052 at 53.991 kb on + strand, within Atu0052 at 53.999 kb on - strand, within Atu0052 at 54.001 kb on - strand, within Atu0052 at 54.068 kb on + strand, within Atu0052 at 54.130 kb on - strand, within Atu0052 at 54.216 kb on + strand, within Atu0052 at 54.216 kb on + strand, within Atu0052 at 54.216 kb on + strand, within Atu0052 at 54.224 kb on - strand, within Atu0052 at 54.224 kb on - strand, within Atu0052 at 54.341 kb on - strand, within Atu0052 at 54.417 kb on - strand, within Atu0052 at 54.445 kb on + strand, within Atu0052 at 54.445 kb on + strand, within Atu0052 at 54.453 kb on - strand, within Atu0052 at 54.453 kb on - strand, within Atu0052 at 54.455 kb on + strand, within Atu0052 at 54.489 kb on + strand, within Atu0052 at 54.509 kb on + strand, within Atu0052 at 54.514 kb on + strand, within Atu0052 at 54.542 kb on - strand, within Atu0052
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with cyanide 2.5 mM remove 51,388 + Atu0049 0.70 +0.0 51,396 - Atu0049 0.72 -0.4 51,560 + -0.2 51,568 - -0.8 51,696 - Atu0050 0.17 -3.1 51,771 - Atu0050 0.30 -0.9 51,771 - Atu0050 0.30 -4.0 51,783 + Atu0050 0.32 -1.8 51,817 + Atu0050 0.38 +0.4 51,817 + Atu0050 0.38 -1.7 51,822 + Atu0050 0.39 -1.8 51,890 + Atu0050 0.50 -2.3 51,911 + Atu0050 0.54 -3.1 51,937 - Atu0050 0.58 -1.6 51,939 + Atu0050 0.59 -2.7 51,988 + Atu0050 0.67 -2.2 51,996 - Atu0050 0.68 -0.8 52,006 - Atu0050 0.70 -3.8 52,169 - -2.6 52,262 - -1.5 52,297 - -1.7 52,306 - -2.4 52,343 - -3.4 52,396 + Atu0051 0.11 -1.5 52,441 + Atu0051 0.14 -1.2 52,464 + Atu0051 0.16 -2.1 52,472 - Atu0051 0.17 +0.1 52,472 - Atu0051 0.17 -0.4 52,491 + Atu0051 0.18 -1.8 52,510 + Atu0051 0.19 -4.0 52,653 + Atu0051 0.30 -0.4 52,796 + Atu0051 0.41 -2.1 52,913 + Atu0051 0.50 -2.5 53,157 + Atu0051 0.69 +0.7 53,227 + Atu0051 0.74 -3.0 53,235 - Atu0051 0.75 -3.0 53,272 - Atu0051 0.78 -1.0 53,292 + Atu0051 0.79 -2.4 53,300 - Atu0051 0.80 -1.5 53,300 - Atu0051 0.80 -1.6 53,377 + Atu0051 0.86 -3.6 53,420 + Atu0051 0.89 +0.1 53,505 + -1.8 53,505 + -2.9 53,505 + -2.5 53,513 - -1.5 53,513 - -0.4 53,513 - -2.4 53,571 + +0.4 53,572 - +0.3 53,579 - +1.8 53,579 - +0.1 53,579 - +1.9 53,606 + -0.3 53,614 - -0.3 53,693 + -0.1 53,738 + -0.2 53,738 + -0.1 53,738 + -0.6 53,746 - -0.4 53,750 + +0.4 53,760 + -0.2 53,760 + +0.4 53,875 + Atu0052 0.11 +0.3 53,919 - Atu0052 0.13 -0.4 53,930 - Atu0052 0.14 -0.2 53,991 + Atu0052 0.16 -0.5 53,991 + Atu0052 0.16 +0.5 53,999 - Atu0052 0.16 -0.1 54,001 - Atu0052 0.16 +0.1 54,068 + Atu0052 0.19 -0.3 54,130 - Atu0052 0.22 +0.6 54,216 + Atu0052 0.25 +0.6 54,216 + Atu0052 0.25 +0.0 54,216 + Atu0052 0.25 -0.3 54,224 - Atu0052 0.25 +1.5 54,224 - Atu0052 0.25 +0.3 54,341 - Atu0052 0.30 +0.2 54,417 - Atu0052 0.33 +0.4 54,445 + Atu0052 0.34 +0.3 54,445 + Atu0052 0.34 +0.5 54,453 - Atu0052 0.35 +0.1 54,453 - Atu0052 0.35 +0.7 54,455 + Atu0052 0.35 +0.6 54,489 + Atu0052 0.36 +0.5 54,509 + Atu0052 0.37 -0.1 54,514 + Atu0052 0.37 -0.5 54,542 - Atu0052 0.38 -0.4
Or see this region's nucleotide sequence