Experiment: R2A with EDTA 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu8196 and Atu4108 are separated by 84 nucleotides Atu4108 and Atu4109 are separated by 70 nucleotides Atu4109 and Atu4110 are separated by 13 nucleotides Atu4110 and Atu4111 are separated by 54 nucleotides
Atu8196: Atu8196 - hypothetical protein, at 1,226,713 to 1,227,942
Atu8196
Atu4108: Atu4108 - ribonuclease D, at 1,228,027 to 1,228,653
Atu4108
Atu4109: Atu4109 - hypothetical protein, at 1,228,724 to 1,229,413
Atu4109
Atu4110: Atu4110 - serine dehydrogenase, at 1,229,427 to 1,230,176
Atu4110
Atu4111: Atu4111 - hypothetical protein, at 1,230,231 to 1,230,494
Atu4111
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-2
-1
0
1 at 1227.748 kb on - strand, within Atu8196 at 1227.758 kb on - strand, within Atu8196 at 1227.758 kb on - strand, within Atu8196 at 1227.784 kb on + strand, within Atu8196 at 1227.784 kb on + strand, within Atu8196 at 1227.882 kb on - strand at 1227.906 kb on + strand at 1227.961 kb on + strand at 1227.969 kb on - strand at 1228.053 kb on + strand at 1228.092 kb on - strand, within Atu4108 at 1228.119 kb on - strand, within Atu4108 at 1228.190 kb on - strand, within Atu4108 at 1228.204 kb on - strand, within Atu4108 at 1228.214 kb on - strand, within Atu4108 at 1228.248 kb on - strand, within Atu4108 at 1228.305 kb on + strand, within Atu4108 at 1228.318 kb on - strand, within Atu4108 at 1228.327 kb on - strand, within Atu4108 at 1228.376 kb on + strand, within Atu4108 at 1228.376 kb on - strand, within Atu4108 at 1228.384 kb on - strand, within Atu4108 at 1228.403 kb on + strand, within Atu4108 at 1228.464 kb on - strand, within Atu4108 at 1228.486 kb on - strand, within Atu4108 at 1228.647 kb on - strand at 1228.657 kb on - strand at 1228.896 kb on - strand, within Atu4109 at 1229.095 kb on - strand, within Atu4109 at 1229.112 kb on - strand, within Atu4109 at 1229.155 kb on + strand, within Atu4109 at 1229.188 kb on + strand, within Atu4109 at 1229.246 kb on + strand, within Atu4109 at 1229.333 kb on + strand, within Atu4109 at 1229.439 kb on + strand at 1229.472 kb on - strand at 1229.652 kb on - strand, within Atu4110 at 1229.652 kb on - strand, within Atu4110 at 1229.654 kb on + strand, within Atu4110 at 1229.680 kb on + strand, within Atu4110 at 1229.734 kb on + strand, within Atu4110 at 1229.768 kb on - strand, within Atu4110 at 1229.775 kb on - strand, within Atu4110 at 1229.778 kb on - strand, within Atu4110 at 1229.803 kb on - strand, within Atu4110 at 1229.849 kb on - strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.865 kb on + strand, within Atu4110 at 1229.873 kb on - strand, within Atu4110 at 1229.914 kb on + strand, within Atu4110 at 1229.995 kb on - strand, within Atu4110 at 1230.012 kb on + strand, within Atu4110 at 1230.115 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with EDTA 0.25 mM remove 1,227,748 - Atu8196 0.84 -0.1 1,227,758 - Atu8196 0.85 +0.2 1,227,758 - Atu8196 0.85 +0.1 1,227,784 + Atu8196 0.87 -0.5 1,227,784 + Atu8196 0.87 +1.0 1,227,882 - +0.0 1,227,906 + -0.1 1,227,961 + -0.3 1,227,969 - +0.5 1,228,053 + -0.7 1,228,092 - Atu4108 0.10 -0.6 1,228,119 - Atu4108 0.15 -2.0 1,228,190 - Atu4108 0.26 -2.4 1,228,204 - Atu4108 0.28 +0.6 1,228,214 - Atu4108 0.30 -1.4 1,228,248 - Atu4108 0.35 -1.1 1,228,305 + Atu4108 0.44 -2.3 1,228,318 - Atu4108 0.46 -2.6 1,228,327 - Atu4108 0.48 -2.1 1,228,376 + Atu4108 0.56 -2.1 1,228,376 - Atu4108 0.56 -2.1 1,228,384 - Atu4108 0.57 -1.2 1,228,403 + Atu4108 0.60 -2.4 1,228,464 - Atu4108 0.70 -2.3 1,228,486 - Atu4108 0.73 -2.3 1,228,647 - -1.1 1,228,657 - +0.7 1,228,896 - Atu4109 0.25 +0.2 1,229,095 - Atu4109 0.54 -0.2 1,229,112 - Atu4109 0.56 +0.3 1,229,155 + Atu4109 0.62 +0.5 1,229,188 + Atu4109 0.67 +0.4 1,229,246 + Atu4109 0.76 -0.0 1,229,333 + Atu4109 0.88 +0.3 1,229,439 + +0.7 1,229,472 - +1.2 1,229,652 - Atu4110 0.30 +0.3 1,229,652 - Atu4110 0.30 -0.2 1,229,654 + Atu4110 0.30 -0.5 1,229,680 + Atu4110 0.34 -0.1 1,229,734 + Atu4110 0.41 -0.2 1,229,768 - Atu4110 0.45 -0.0 1,229,775 - Atu4110 0.46 -0.8 1,229,778 - Atu4110 0.47 +0.9 1,229,803 - Atu4110 0.50 +0.3 1,229,849 - Atu4110 0.56 +0.4 1,229,865 + Atu4110 0.58 +0.3 1,229,865 + Atu4110 0.58 -0.3 1,229,865 + Atu4110 0.58 +0.0 1,229,873 - Atu4110 0.59 -0.3 1,229,914 + Atu4110 0.65 -0.0 1,229,995 - Atu4110 0.76 -1.8 1,230,012 + Atu4110 0.78 -0.2 1,230,115 + -0.1
Or see this region's nucleotide sequence