Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14505 and PS417_14510 are separated by 1 nucleotides PS417_14510 and PS417_14515 overlap by 4 nucleotides PS417_14515 and PS417_14520 overlap by 4 nucleotides PS417_14520 and PS417_14525 overlap by 4 nucleotides
PS417_14505: PS417_14505 - glycogen debranching protein, at 3,171,398 to 3,173,557
_14505
PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557
_14510
PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312
_14515
PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908
_14520
PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564
_14525
Position (kb) 3174
3175
3176 Strain fitness (log2 ratio) -1
0
1 at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.472 kb on - strand at 3174.472 kb on - strand at 3174.969 kb on - strand, within PS417_14515 at 3175.309 kb on - strand at 3175.436 kb on - strand, within PS417_14520 at 3175.638 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 3,174,471 + -0.1 3,174,471 + +0.2 3,174,471 + -0.1 3,174,472 - +0.0 3,174,472 - +0.0 3,174,969 - PS417_14515 0.55 -0.6 3,175,309 - -0.1 3,175,436 - PS417_14520 0.21 +0.0 3,175,638 - PS417_14520 0.55 -0.0 3,175,655 - PS417_14520 0.58 -0.4 3,175,655 - PS417_14520 0.58 -0.3
Or see this region's nucleotide sequence