Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11515 and PS417_11520 are separated by 18 nucleotides PS417_11520 and PS417_11525 are separated by 18 nucleotides PS417_11525 and PS417_11530 are separated by 147 nucleotides
PS417_11515: PS417_11515 - sugar ABC transporter substrate-binding protein, at 2,476,415 to 2,477,725
_11515
PS417_11520: PS417_11520 - Sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,477,744 to 2,478,517
_11520
PS417_11525: PS417_11525 - ADP-ribose pyrophosphatase, at 2,478,536 to 2,479,228
_11525
PS417_11530: PS417_11530 - amidase, at 2,479,376 to 2,480,023
_11530
Position (kb)
2477
2478
2479 Strain fitness (log2 ratio)
-1
0
1
2 at 2476.800 kb on - strand at 2476.835 kb on + strand, within PS417_11515 at 2476.889 kb on - strand, within PS417_11515 at 2476.987 kb on + strand, within PS417_11515 at 2477.094 kb on - strand, within PS417_11515 at 2477.114 kb on + strand, within PS417_11515 at 2477.310 kb on - strand, within PS417_11515 at 2477.393 kb on + strand, within PS417_11515 at 2477.393 kb on + strand, within PS417_11515 at 2477.394 kb on - strand, within PS417_11515 at 2477.394 kb on - strand, within PS417_11515 at 2477.460 kb on - strand, within PS417_11515 at 2477.460 kb on - strand at 2477.573 kb on + strand, within PS417_11515 at 2477.574 kb on - strand, within PS417_11515 at 2477.647 kb on + strand at 2477.820 kb on - strand at 2478.053 kb on + strand, within PS417_11520 at 2478.200 kb on + strand, within PS417_11520 at 2478.200 kb on + strand, within PS417_11520 at 2478.200 kb on + strand, within PS417_11520 at 2478.467 kb on + strand at 2478.485 kb on + strand at 2478.486 kb on - strand at 2479.281 kb on - strand at 2479.304 kb on - strand at 2479.496 kb on + strand, within PS417_11530 at 2479.497 kb on - strand, within PS417_11530 at 2479.497 kb on - strand, within PS417_11530
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 2,476,800 - +0.7 2,476,835 + PS417_11515 0.32 +0.1 2,476,889 - PS417_11515 0.36 +2.3 2,476,987 + PS417_11515 0.44 -0.8 2,477,094 - PS417_11515 0.52 -0.1 2,477,114 + PS417_11515 0.53 +0.1 2,477,310 - PS417_11515 0.68 +0.1 2,477,393 + PS417_11515 0.75 +0.4 2,477,393 + PS417_11515 0.75 +0.3 2,477,394 - PS417_11515 0.75 -0.1 2,477,394 - PS417_11515 0.75 -0.6 2,477,460 - PS417_11515 0.80 +0.4 2,477,460 - +0.7 2,477,573 + PS417_11515 0.88 -0.6 2,477,574 - PS417_11515 0.88 -0.0 2,477,647 + +0.7 2,477,820 - +0.2 2,478,053 + PS417_11520 0.40 -0.1 2,478,200 + PS417_11520 0.59 -1.2 2,478,200 + PS417_11520 0.59 -1.0 2,478,200 + PS417_11520 0.59 -0.7 2,478,467 + -0.3 2,478,485 + -0.5 2,478,486 - -1.3 2,479,281 - +0.0 2,479,304 - -0.5 2,479,496 + PS417_11530 0.19 -0.1 2,479,497 - PS417_11530 0.19 -0.1 2,479,497 - PS417_11530 0.19 +0.8
Or see this region's nucleotide sequence