Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08130 and PS417_08135 are separated by 28 nucleotides PS417_08135 and PS417_08140 overlap by 4 nucleotides PS417_08140 and PS417_08145 are separated by 120 nucleotides
PS417_08130: PS417_08130 - aspartate aminotransferase, at 1,780,167 to 1,781,279
_08130
PS417_08135: PS417_08135 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,781,308 to 1,783,518
_08135
PS417_08140: PS417_08140 - cytidylate kinase, at 1,783,515 to 1,784,204
_08140
PS417_08145: PS417_08145 - 30S ribosomal protein S1, at 1,784,325 to 1,786,010
_08145
Position (kb)
1781
1782
1783
1784 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1780.764 kb on + strand, within PS417_08130 at 1780.764 kb on + strand, within PS417_08130 at 1780.764 kb on + strand, within PS417_08130 at 1780.860 kb on + strand, within PS417_08130 at 1781.184 kb on + strand at 1781.211 kb on - strand at 1781.211 kb on - strand at 1781.307 kb on + strand at 1781.307 kb on + strand at 1781.307 kb on + strand at 1781.308 kb on - strand at 1781.827 kb on - strand, within PS417_08135 at 1781.919 kb on + strand, within PS417_08135 at 1782.113 kb on + strand, within PS417_08135 at 1782.113 kb on + strand, within PS417_08135 at 1782.182 kb on - strand, within PS417_08135 at 1782.691 kb on + strand, within PS417_08135 at 1782.691 kb on + strand, within PS417_08135 at 1782.798 kb on + strand, within PS417_08135 at 1782.798 kb on + strand, within PS417_08135 at 1782.909 kb on + strand, within PS417_08135 at 1782.911 kb on + strand, within PS417_08135 at 1782.911 kb on + strand, within PS417_08135 at 1782.912 kb on - strand, within PS417_08135 at 1783.314 kb on + strand at 1783.446 kb on + strand at 1783.467 kb on + strand at 1783.467 kb on + strand at 1784.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 1,780,764 + PS417_08130 0.54 -2.6 1,780,764 + PS417_08130 0.54 -1.8 1,780,764 + PS417_08130 0.54 +1.0 1,780,860 + PS417_08130 0.62 -1.5 1,781,184 + -0.5 1,781,211 - -1.2 1,781,211 - -2.1 1,781,307 + -0.0 1,781,307 + -1.4 1,781,307 + -0.7 1,781,308 - -1.2 1,781,827 - PS417_08135 0.23 -1.8 1,781,919 + PS417_08135 0.28 -4.1 1,782,113 + PS417_08135 0.36 -1.0 1,782,113 + PS417_08135 0.36 +0.6 1,782,182 - PS417_08135 0.40 +0.1 1,782,691 + PS417_08135 0.63 -0.6 1,782,691 + PS417_08135 0.63 -0.1 1,782,798 + PS417_08135 0.67 -0.1 1,782,798 + PS417_08135 0.67 +0.8 1,782,909 + PS417_08135 0.72 -1.7 1,782,911 + PS417_08135 0.73 -0.2 1,782,911 + PS417_08135 0.73 -1.0 1,782,912 - PS417_08135 0.73 -1.7 1,783,314 + -0.8 1,783,446 + -0.5 1,783,467 + -0.1 1,783,467 + +0.0 1,784,206 + -0.7
Or see this region's nucleotide sequence