Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23200 and PS417_23205 are separated by 50 nucleotides PS417_23205 and PS417_23210 are separated by 291 nucleotides PS417_23210 and PS417_23215 are separated by 2 nucleotides PS417_23215 and PS417_23220 are separated by 145 nucleotides
PS417_23200: PS417_23200 - cysteine desulfurase, at 5,025,811 to 5,027,025
_23200
PS417_23205: PS417_23205 - transcriptional regulator, at 5,027,076 to 5,027,567
_23205
PS417_23210: PS417_23210 - serine acetyltransferase, at 5,027,859 to 5,028,641
_23210
PS417_23215: PS417_23215 - RNA methyltransferase, at 5,028,644 to 5,029,414
_23215
PS417_23220: PS417_23220 - inositol monophosphatase, at 5,029,560 to 5,030,375
_23220
Position (kb)
5027
5028
5029 Strain fitness (log2 ratio)
-1
0
1
2 at 5027.349 kb on - strand, within PS417_23205 at 5027.447 kb on - strand, within PS417_23205 at 5027.576 kb on - strand at 5027.576 kb on - strand at 5027.576 kb on - strand at 5027.870 kb on - strand at 5028.065 kb on - strand, within PS417_23210 at 5028.658 kb on + strand at 5028.710 kb on + strand at 5028.712 kb on + strand at 5028.712 kb on + strand at 5028.713 kb on - strand at 5028.713 kb on - strand at 5028.713 kb on - strand at 5028.835 kb on - strand, within PS417_23215 at 5028.835 kb on - strand, within PS417_23215 at 5028.835 kb on - strand, within PS417_23215 at 5028.902 kb on + strand, within PS417_23215 at 5028.903 kb on - strand, within PS417_23215 at 5028.951 kb on + strand, within PS417_23215 at 5029.381 kb on + strand at 5029.472 kb on - strand at 5029.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 5,027,349 - PS417_23205 0.55 -0.2 5,027,447 - PS417_23205 0.75 -0.2 5,027,576 - +2.3 5,027,576 - +0.7 5,027,576 - +0.3 5,027,870 - -0.1 5,028,065 - PS417_23210 0.26 -0.6 5,028,658 + +0.0 5,028,710 + +0.2 5,028,712 + -1.1 5,028,712 + -0.6 5,028,713 - +2.6 5,028,713 - -0.6 5,028,713 - +0.7 5,028,835 - PS417_23215 0.25 -0.4 5,028,835 - PS417_23215 0.25 -0.2 5,028,835 - PS417_23215 0.25 -0.7 5,028,902 + PS417_23215 0.33 +0.2 5,028,903 - PS417_23215 0.34 -0.3 5,028,951 + PS417_23215 0.40 -0.4 5,029,381 + -0.4 5,029,472 - +0.7 5,029,486 - -0.1
Or see this region's nucleotide sequence