Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10300 and PS417_10305 are separated by 224 nucleotides PS417_10305 and PS417_10310 are separated by 42 nucleotides PS417_10310 and PS417_10315 are separated by 31 nucleotides
PS417_10300: PS417_10300 - protease, at 2,203,199 to 2,204,512
_10300
PS417_10305: PS417_10305 - hypothetical protein, at 2,204,737 to 2,205,000
_10305
PS417_10310: PS417_10310 - hypothetical protein, at 2,205,043 to 2,205,255
_10310
PS417_10315: PS417_10315 - cytochrome C biogenesis protein, at 2,205,287 to 2,206,495
_10315
Position (kb)
2204
2205
2206 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2203.743 kb on + strand at 2203.903 kb on - strand, within PS417_10300 at 2203.957 kb on + strand, within PS417_10300 at 2203.959 kb on + strand at 2203.986 kb on + strand, within PS417_10300 at 2203.987 kb on - strand, within PS417_10300 at 2204.072 kb on + strand, within PS417_10300 at 2204.073 kb on - strand, within PS417_10300 at 2204.624 kb on - strand at 2204.680 kb on + strand at 2204.680 kb on + strand at 2204.680 kb on + strand at 2204.681 kb on - strand at 2204.681 kb on - strand at 2204.756 kb on - strand at 2204.840 kb on - strand, within PS417_10305 at 2204.926 kb on + strand, within PS417_10305 at 2204.963 kb on - strand, within PS417_10305 at 2205.291 kb on + strand at 2205.338 kb on - strand at 2205.471 kb on + strand, within PS417_10315 at 2205.472 kb on - strand, within PS417_10315 at 2205.481 kb on + strand, within PS417_10315 at 2205.924 kb on + strand, within PS417_10315
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 2,203,743 + -1.2 2,203,903 - PS417_10300 0.54 +0.3 2,203,957 + PS417_10300 0.58 -0.3 2,203,959 + +3.6 2,203,986 + PS417_10300 0.60 -0.4 2,203,987 - PS417_10300 0.60 +0.3 2,204,072 + PS417_10300 0.66 -1.7 2,204,073 - PS417_10300 0.67 +0.5 2,204,624 - +0.4 2,204,680 + -1.6 2,204,680 + +0.4 2,204,680 + -0.3 2,204,681 - -1.1 2,204,681 - +0.4 2,204,756 - -0.6 2,204,840 - PS417_10305 0.39 -0.7 2,204,926 + PS417_10305 0.72 -0.9 2,204,963 - PS417_10305 0.86 -1.2 2,205,291 + +0.1 2,205,338 - -0.1 2,205,471 + PS417_10315 0.15 -1.1 2,205,472 - PS417_10315 0.15 -0.2 2,205,481 + PS417_10315 0.16 +0.2 2,205,924 + PS417_10315 0.53 +0.2
Or see this region's nucleotide sequence