Experiment: Phosphomycin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25775 and PS417_25780 are separated by 71 nucleotides PS417_25780 and PS417_25785 are separated by 1 nucleotides PS417_25785 and PS417_25790 overlap by 4 nucleotides
PS417_25775: PS417_25775 - lipoprotein, at 5,565,103 to 5,566,077
_25775
PS417_25780: PS417_25780 - molecular chaperone DnaJ, at 5,566,149 to 5,566,916
_25780
PS417_25785: PS417_25785 - mannose-1-phosphate guanylyltransferase, at 5,566,918 to 5,567,592
_25785
PS417_25790: PS417_25790 - aminoglycoside phosphotransferase, at 5,567,589 to 5,568,614
_25790
Position (kb) 5566
5567
5568 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5565.971 kb on - strand, within PS417_25775 at 5566.031 kb on - strand at 5566.127 kb on + strand at 5566.183 kb on + strand at 5566.184 kb on - strand at 5566.205 kb on + strand at 5566.357 kb on + strand, within PS417_25780 at 5566.362 kb on - strand, within PS417_25780 at 5566.527 kb on + strand, within PS417_25780 at 5566.566 kb on + strand, within PS417_25780 at 5566.567 kb on - strand, within PS417_25780 at 5566.570 kb on + strand, within PS417_25780 at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.593 kb on - strand at 5567.677 kb on - strand at 5567.678 kb on + strand at 5567.679 kb on - strand at 5567.679 kb on - strand at 5567.680 kb on + strand at 5567.680 kb on + strand at 5567.932 kb on + strand, within PS417_25790 at 5567.933 kb on - strand, within PS417_25790 at 5567.948 kb on - strand, within PS417_25790 at 5568.011 kb on + strand, within PS417_25790 at 5568.011 kb on + strand at 5568.013 kb on + strand, within PS417_25790 at 5568.013 kb on + strand, within PS417_25790 at 5568.134 kb on - strand, within PS417_25790 at 5568.182 kb on - strand, within PS417_25790 at 5568.182 kb on - strand, within PS417_25790 at 5568.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.001 mg/ml remove 5,565,971 - PS417_25775 0.89 +0.2 5,566,031 - -0.9 5,566,127 + -0.1 5,566,183 + +0.9 5,566,184 - +0.5 5,566,205 + -0.1 5,566,357 + PS417_25780 0.27 +0.4 5,566,362 - PS417_25780 0.28 +0.0 5,566,527 + PS417_25780 0.49 +1.3 5,566,566 + PS417_25780 0.54 +0.6 5,566,567 - PS417_25780 0.54 -0.1 5,566,570 + PS417_25780 0.55 -0.2 5,567,593 - -1.0 5,567,593 - -0.9 5,567,593 - +0.7 5,567,593 - -0.3 5,567,677 - -2.4 5,567,678 + -2.3 5,567,679 - -0.7 5,567,679 - -0.5 5,567,680 + -3.2 5,567,680 + -2.2 5,567,932 + PS417_25790 0.33 +1.1 5,567,933 - PS417_25790 0.34 -2.3 5,567,948 - PS417_25790 0.35 +0.1 5,568,011 + PS417_25790 0.41 -1.0 5,568,011 + +0.7 5,568,013 + PS417_25790 0.41 -2.7 5,568,013 + PS417_25790 0.41 -2.1 5,568,134 - PS417_25790 0.53 -3.0 5,568,182 - PS417_25790 0.58 -1.2 5,568,182 - PS417_25790 0.58 -1.5 5,568,591 + -1.1
Or see this region's nucleotide sequence