Experiment: Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14040 and PS417_14045 are separated by 127 nucleotides PS417_14045 and PS417_14050 are separated by 143 nucleotides PS417_14050 and PS417_14055 are separated by 170 nucleotides
PS417_14040: PS417_14040 - alkyl hydroperoxide reductase, at 3,083,501 to 3,085,066
_14040
PS417_14045: PS417_14045 - alkyl hydroperoxide reductase, at 3,085,194 to 3,085,757
_14045
PS417_14050: PS417_14050 - recombinase, at 3,085,901 to 3,086,827
_14050
PS417_14055: PS417_14055 - integrase, at 3,086,998 to 3,088,005
_14055
Position (kb)
3085
3086
3087 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3085.030 kb on - strand at 3085.048 kb on + strand at 3085.048 kb on + strand at 3085.049 kb on - strand at 3085.067 kb on - strand at 3085.094 kb on - strand at 3085.156 kb on - strand at 3085.232 kb on + strand at 3085.233 kb on - strand at 3085.289 kb on - strand, within PS417_14045 at 3085.289 kb on - strand, within PS417_14045 at 3085.394 kb on + strand, within PS417_14045 at 3085.477 kb on + strand at 3085.478 kb on - strand, within PS417_14045 at 3085.585 kb on + strand, within PS417_14045 at 3085.585 kb on + strand, within PS417_14045 at 3085.643 kb on - strand, within PS417_14045 at 3085.693 kb on - strand, within PS417_14045 at 3085.711 kb on + strand at 3085.734 kb on + strand at 3085.781 kb on + strand at 3085.782 kb on - strand at 3085.791 kb on + strand at 3085.888 kb on - strand at 3085.992 kb on + strand at 3086.070 kb on + strand, within PS417_14050 at 3086.268 kb on + strand, within PS417_14050 at 3086.269 kb on - strand, within PS417_14050 at 3086.876 kb on + strand at 3086.877 kb on - strand at 3086.891 kb on + strand at 3086.891 kb on + strand at 3086.963 kb on - strand at 3087.087 kb on - strand at 3087.087 kb on - strand at 3087.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.01 mg/ml remove 3,085,030 - +0.8 3,085,048 + -2.3 3,085,048 + +0.5 3,085,049 - +0.0 3,085,067 - +1.0 3,085,094 - +0.4 3,085,156 - -0.0 3,085,232 + -0.1 3,085,233 - +3.8 3,085,289 - PS417_14045 0.17 +1.0 3,085,289 - PS417_14045 0.17 -1.6 3,085,394 + PS417_14045 0.35 +1.8 3,085,477 + +1.0 3,085,478 - PS417_14045 0.50 -0.6 3,085,585 + PS417_14045 0.69 -1.0 3,085,585 + PS417_14045 0.69 -1.3 3,085,643 - PS417_14045 0.80 +1.4 3,085,693 - PS417_14045 0.88 -0.4 3,085,711 + +0.4 3,085,734 + +0.1 3,085,781 + +1.0 3,085,782 - +0.3 3,085,791 + +0.2 3,085,888 - -0.6 3,085,992 + -0.0 3,086,070 + PS417_14050 0.18 -1.5 3,086,268 + PS417_14050 0.40 -0.8 3,086,269 - PS417_14050 0.40 -0.5 3,086,876 + -0.3 3,086,877 - -0.3 3,086,891 + -1.3 3,086,891 + +0.1 3,086,963 - -0.3 3,087,087 - -0.7 3,087,087 - +0.1 3,087,743 + +1.0
Or see this region's nucleotide sequence