Experiment: Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13495 and PS417_13500 overlap by 4 nucleotides PS417_13500 and PS417_13505 are separated by 80 nucleotides
PS417_13495: PS417_13495 - nuclease, at 2,973,412 to 2,975,058
_13495
PS417_13500: PS417_13500 - ATP-dependent DNA helicase, at 2,975,055 to 2,977,310
_13500
PS417_13505: PS417_13505 - alpha/beta hydrolase, at 2,977,391 to 2,978,323
_13505
Position (kb)
2975
2976
2977
2978 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2974.122 kb on + strand, within PS417_13495 at 2974.358 kb on + strand, within PS417_13495 at 2974.738 kb on + strand, within PS417_13495 at 2975.061 kb on + strand at 2975.062 kb on - strand at 2975.197 kb on - strand at 2975.216 kb on - strand at 2975.216 kb on - strand at 2975.302 kb on - strand at 2975.344 kb on + strand, within PS417_13500 at 2975.344 kb on + strand, within PS417_13500 at 2975.345 kb on - strand, within PS417_13500 at 2975.345 kb on - strand, within PS417_13500 at 2975.368 kb on - strand, within PS417_13500 at 2975.424 kb on + strand, within PS417_13500 at 2975.637 kb on + strand at 2975.706 kb on - strand, within PS417_13500 at 2975.934 kb on + strand, within PS417_13500 at 2975.935 kb on - strand, within PS417_13500 at 2976.085 kb on - strand, within PS417_13500 at 2976.090 kb on + strand, within PS417_13500 at 2976.090 kb on + strand at 2976.093 kb on + strand, within PS417_13500 at 2976.094 kb on - strand, within PS417_13500 at 2976.094 kb on - strand, within PS417_13500 at 2976.222 kb on + strand, within PS417_13500 at 2976.327 kb on + strand, within PS417_13500 at 2976.336 kb on + strand, within PS417_13500 at 2976.399 kb on + strand, within PS417_13500 at 2976.400 kb on - strand, within PS417_13500 at 2976.400 kb on - strand, within PS417_13500 at 2976.651 kb on + strand, within PS417_13500 at 2976.655 kb on - strand, within PS417_13500 at 2976.727 kb on + strand, within PS417_13500 at 2976.727 kb on + strand, within PS417_13500 at 2976.727 kb on + strand, within PS417_13500 at 2976.728 kb on - strand, within PS417_13500 at 2976.816 kb on + strand, within PS417_13500 at 2976.838 kb on - strand, within PS417_13500 at 2976.838 kb on - strand, within PS417_13500 at 2976.838 kb on - strand, within PS417_13500 at 2976.886 kb on + strand, within PS417_13500 at 2977.094 kb on - strand at 2977.155 kb on + strand at 2977.155 kb on + strand at 2977.156 kb on - strand at 2977.161 kb on + strand at 2977.161 kb on + strand at 2977.162 kb on - strand at 2977.162 kb on - strand at 2977.428 kb on + strand at 2977.438 kb on + strand at 2977.624 kb on - strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2978.027 kb on + strand, within PS417_13505 at 2978.130 kb on + strand, within PS417_13505 at 2978.238 kb on + strand at 2978.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.01 mg/ml remove 2,974,122 + PS417_13495 0.43 -1.5 2,974,358 + PS417_13495 0.57 -0.8 2,974,738 + PS417_13495 0.81 -2.7 2,975,061 + -1.3 2,975,062 - -1.5 2,975,197 - -2.8 2,975,216 - -3.2 2,975,216 - -2.5 2,975,302 - -0.6 2,975,344 + PS417_13500 0.13 -3.4 2,975,344 + PS417_13500 0.13 -3.2 2,975,345 - PS417_13500 0.13 -0.8 2,975,345 - PS417_13500 0.13 -1.7 2,975,368 - PS417_13500 0.14 -2.5 2,975,424 + PS417_13500 0.16 -2.2 2,975,637 + +0.0 2,975,706 - PS417_13500 0.29 -2.5 2,975,934 + PS417_13500 0.39 -2.2 2,975,935 - PS417_13500 0.39 -3.7 2,976,085 - PS417_13500 0.46 -2.4 2,976,090 + PS417_13500 0.46 -2.0 2,976,090 + +1.0 2,976,093 + PS417_13500 0.46 -2.6 2,976,094 - PS417_13500 0.46 -1.9 2,976,094 - PS417_13500 0.46 -3.9 2,976,222 + PS417_13500 0.52 +0.0 2,976,327 + PS417_13500 0.56 -2.6 2,976,336 + PS417_13500 0.57 -2.0 2,976,399 + PS417_13500 0.60 -2.7 2,976,400 - PS417_13500 0.60 -2.3 2,976,400 - PS417_13500 0.60 -3.2 2,976,651 + PS417_13500 0.71 -1.0 2,976,655 - PS417_13500 0.71 +1.0 2,976,727 + PS417_13500 0.74 -1.5 2,976,727 + PS417_13500 0.74 -4.1 2,976,727 + PS417_13500 0.74 -3.8 2,976,728 - PS417_13500 0.74 -2.7 2,976,816 + PS417_13500 0.78 -1.8 2,976,838 - PS417_13500 0.79 -1.3 2,976,838 - PS417_13500 0.79 -2.3 2,976,838 - PS417_13500 0.79 -0.6 2,976,886 + PS417_13500 0.81 -1.6 2,977,094 - -1.0 2,977,155 + -3.4 2,977,155 + -3.2 2,977,156 - -1.8 2,977,161 + -2.2 2,977,161 + -3.0 2,977,162 - -2.2 2,977,162 - -1.3 2,977,428 + +0.0 2,977,438 + +0.2 2,977,624 - PS417_13505 0.25 -0.2 2,977,835 + PS417_13505 0.48 +1.5 2,977,835 + PS417_13505 0.48 +1.4 2,977,835 + PS417_13505 0.48 +0.5 2,977,836 - PS417_13505 0.48 +1.4 2,977,836 - PS417_13505 0.48 +0.5 2,977,836 - PS417_13505 0.48 +1.0 2,978,027 + PS417_13505 0.68 +0.3 2,978,130 + PS417_13505 0.79 +1.0 2,978,238 + +0.9 2,978,238 + -0.3
Or see this region's nucleotide sequence