Experiment: Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12225 and PS417_12230 are separated by 30 nucleotides PS417_12230 and PS417_12235 are separated by 64 nucleotides PS417_12235 and PS417_12240 are separated by 127 nucleotides
PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505
_12225
PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988
_12230
PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799
_12235
PS417_12240: PS417_12240 - lysine decarboxylase, at 2,659,927 to 2,662,182
_12240
Position (kb)
2659
2660 Strain fitness (log2 ratio)
-1
0
1 at 2659.004 kb on + strand at 2659.007 kb on + strand at 2659.833 kb on + strand at 2659.834 kb on - strand at 2659.889 kb on + strand at 2659.916 kb on + strand at 2660.142 kb on + strand at 2660.143 kb on - strand at 2660.233 kb on - strand, within PS417_12240 at 2660.354 kb on - strand, within PS417_12240 at 2660.354 kb on - strand, within PS417_12240 at 2660.416 kb on + strand, within PS417_12240 at 2660.416 kb on + strand, within PS417_12240 at 2660.764 kb on + strand, within PS417_12240 at 2660.764 kb on + strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.01 mg/ml remove 2,659,004 + +0.3 2,659,007 + +1.2 2,659,833 + -0.5 2,659,834 - +0.4 2,659,889 + -1.4 2,659,916 + -1.3 2,660,142 + +0.4 2,660,143 - +0.0 2,660,233 - PS417_12240 0.14 -0.3 2,660,354 - PS417_12240 0.19 +0.4 2,660,354 - PS417_12240 0.19 -0.8 2,660,416 + PS417_12240 0.22 -0.7 2,660,416 + PS417_12240 0.22 -1.0 2,660,764 + PS417_12240 0.37 +0.0 2,660,764 + PS417_12240 0.37 -0.0 2,660,765 - PS417_12240 0.37 -0.4 2,660,765 - PS417_12240 0.37 -0.5 2,660,765 - PS417_12240 0.37 -0.3
Or see this region's nucleotide sequence