Experiment: R2A with Novobiocin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4722 and Atu4723 are separated by 4 nucleotides Atu4723 and Atu4724 are separated by 70 nucleotides Atu4724 and Atu8200 are separated by 182 nucleotides Atu8200 and Atu4726 are separated by 193 nucleotides
Atu4722: Atu4722 - gamma-glutamyltranspeptidase, at 1,884,541 to 1,886,325
Atu4722
Atu4723: Atu4723 - ABC transporter permease, at 1,886,330 to 1,888,141
Atu4723
Atu4724: Atu4724 - hypothetical protein, at 1,888,212 to 1,888,415
Atu4724
Atu8200: Atu8200 - hypothetical protein, at 1,888,598 to 1,888,918
Atu8200
Atu4726: Atu4726 - superoxide dismutase, at 1,889,112 to 1,889,807
Atu4726
Position (kb)
1886
1887
1888
1889 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1885.384 kb on - strand, within Atu4722 at 1885.384 kb on - strand, within Atu4722 at 1885.388 kb on + strand, within Atu4722 at 1885.435 kb on + strand, within Atu4722 at 1885.454 kb on + strand, within Atu4722 at 1885.462 kb on - strand, within Atu4722 at 1885.462 kb on - strand, within Atu4722 at 1885.468 kb on - strand, within Atu4722 at 1885.515 kb on + strand, within Atu4722 at 1885.572 kb on + strand, within Atu4722 at 1885.608 kb on + strand, within Atu4722 at 1885.621 kb on - strand, within Atu4722 at 1885.621 kb on - strand, within Atu4722 at 1885.669 kb on - strand, within Atu4722 at 1885.679 kb on - strand, within Atu4722 at 1885.788 kb on + strand, within Atu4722 at 1885.796 kb on - strand, within Atu4722 at 1885.808 kb on - strand, within Atu4722 at 1885.840 kb on + strand, within Atu4722 at 1885.848 kb on - strand, within Atu4722 at 1885.946 kb on + strand, within Atu4722 at 1885.946 kb on + strand, within Atu4722 at 1885.946 kb on + strand, within Atu4722 at 1885.951 kb on + strand, within Atu4722 at 1885.956 kb on + strand, within Atu4722 at 1885.970 kb on + strand, within Atu4722 at 1885.986 kb on - strand, within Atu4722 at 1886.080 kb on + strand, within Atu4722 at 1886.085 kb on + strand, within Atu4722 at 1886.085 kb on + strand, within Atu4722 at 1886.093 kb on - strand, within Atu4722 at 1886.098 kb on + strand, within Atu4722 at 1886.149 kb on + strand at 1886.177 kb on + strand at 1886.343 kb on - strand at 1886.540 kb on + strand, within Atu4723 at 1886.548 kb on - strand, within Atu4723 at 1886.561 kb on + strand, within Atu4723 at 1886.627 kb on - strand, within Atu4723 at 1886.681 kb on + strand, within Atu4723 at 1886.757 kb on - strand, within Atu4723 at 1887.043 kb on - strand, within Atu4723 at 1887.189 kb on + strand, within Atu4723 at 1887.290 kb on + strand, within Atu4723 at 1887.299 kb on + strand, within Atu4723 at 1887.312 kb on - strand, within Atu4723 at 1887.332 kb on + strand, within Atu4723 at 1887.474 kb on + strand, within Atu4723 at 1887.493 kb on + strand, within Atu4723 at 1887.586 kb on + strand, within Atu4723 at 1887.600 kb on + strand, within Atu4723 at 1887.624 kb on - strand, within Atu4723 at 1887.738 kb on + strand, within Atu4723 at 1887.738 kb on + strand, within Atu4723 at 1887.745 kb on + strand, within Atu4723 at 1887.746 kb on - strand, within Atu4723 at 1887.765 kb on + strand, within Atu4723 at 1887.802 kb on - strand, within Atu4723 at 1887.819 kb on + strand, within Atu4723 at 1887.872 kb on - strand, within Atu4723 at 1887.894 kb on + strand, within Atu4723 at 1887.947 kb on + strand, within Atu4723 at 1887.990 kb on - strand at 1888.107 kb on + strand at 1888.132 kb on + strand at 1888.218 kb on + strand at 1888.226 kb on - strand at 1888.302 kb on - strand, within Atu4724 at 1888.361 kb on + strand, within Atu4724 at 1888.366 kb on + strand, within Atu4724 at 1888.469 kb on + strand at 1888.642 kb on + strand, within Atu8200 at 1888.668 kb on - strand, within Atu8200 at 1888.696 kb on - strand, within Atu8200 at 1888.734 kb on + strand, within Atu8200 at 1888.903 kb on + strand at 1888.911 kb on - strand at 1888.927 kb on + strand at 1888.974 kb on + strand at 1889.057 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Novobiocin 0.002 mM remove 1,885,384 - Atu4722 0.47 +0.4 1,885,384 - Atu4722 0.47 -0.8 1,885,388 + Atu4722 0.47 +0.6 1,885,435 + Atu4722 0.50 -0.6 1,885,454 + Atu4722 0.51 +1.1 1,885,462 - Atu4722 0.52 +0.9 1,885,462 - Atu4722 0.52 +0.3 1,885,468 - Atu4722 0.52 +0.1 1,885,515 + Atu4722 0.55 +0.3 1,885,572 + Atu4722 0.58 +0.9 1,885,608 + Atu4722 0.60 -0.3 1,885,621 - Atu4722 0.61 +1.0 1,885,621 - Atu4722 0.61 -1.0 1,885,669 - Atu4722 0.63 -0.6 1,885,679 - Atu4722 0.64 -0.2 1,885,788 + Atu4722 0.70 +1.9 1,885,796 - Atu4722 0.70 +0.4 1,885,808 - Atu4722 0.71 -1.0 1,885,840 + Atu4722 0.73 +0.5 1,885,848 - Atu4722 0.73 +0.1 1,885,946 + Atu4722 0.79 -0.3 1,885,946 + Atu4722 0.79 -0.2 1,885,946 + Atu4722 0.79 -0.5 1,885,951 + Atu4722 0.79 -0.2 1,885,956 + Atu4722 0.79 +0.6 1,885,970 + Atu4722 0.80 +0.0 1,885,986 - Atu4722 0.81 -0.2 1,886,080 + Atu4722 0.86 +0.2 1,886,085 + Atu4722 0.86 -0.0 1,886,085 + Atu4722 0.86 +0.1 1,886,093 - Atu4722 0.87 -0.8 1,886,098 + Atu4722 0.87 +0.4 1,886,149 + +1.4 1,886,177 + +0.6 1,886,343 - +0.1 1,886,540 + Atu4723 0.12 +1.9 1,886,548 - Atu4723 0.12 +0.4 1,886,561 + Atu4723 0.13 +3.2 1,886,627 - Atu4723 0.16 +0.4 1,886,681 + Atu4723 0.19 +2.2 1,886,757 - Atu4723 0.24 +0.6 1,887,043 - Atu4723 0.39 +0.1 1,887,189 + Atu4723 0.47 +0.1 1,887,290 + Atu4723 0.53 +1.0 1,887,299 + Atu4723 0.53 +0.4 1,887,312 - Atu4723 0.54 +0.4 1,887,332 + Atu4723 0.55 +1.0 1,887,474 + Atu4723 0.63 +0.5 1,887,493 + Atu4723 0.64 -0.1 1,887,586 + Atu4723 0.69 -0.5 1,887,600 + Atu4723 0.70 +0.0 1,887,624 - Atu4723 0.71 -0.6 1,887,738 + Atu4723 0.78 +1.2 1,887,738 + Atu4723 0.78 -0.1 1,887,745 + Atu4723 0.78 +0.3 1,887,746 - Atu4723 0.78 -1.0 1,887,765 + Atu4723 0.79 -0.1 1,887,802 - Atu4723 0.81 +0.5 1,887,819 + Atu4723 0.82 -1.7 1,887,872 - Atu4723 0.85 -0.1 1,887,894 + Atu4723 0.86 +1.5 1,887,947 + Atu4723 0.89 +0.7 1,887,990 - +1.0 1,888,107 + -1.1 1,888,132 + +0.8 1,888,218 + -0.1 1,888,226 - -0.2 1,888,302 - Atu4724 0.44 +2.0 1,888,361 + Atu4724 0.73 -0.1 1,888,366 + Atu4724 0.75 +0.1 1,888,469 + -0.1 1,888,642 + Atu8200 0.14 +0.9 1,888,668 - Atu8200 0.22 +1.3 1,888,696 - Atu8200 0.31 +0.2 1,888,734 + Atu8200 0.42 +0.1 1,888,903 + +0.6 1,888,911 - +0.6 1,888,927 + +0.4 1,888,974 + +0.2 1,889,057 + -0.2
Or see this region's nucleotide sequence