Experiment: R2A with Novobiocin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3871 and Atu3872 are separated by 29 nucleotides Atu3872 and Atu3873 are separated by 195 nucleotides Atu3873 and Atu3874 are separated by 255 nucleotides
Atu3871: Atu3871 - L-lactate dehydrogenase, at 964,886 to 966,031
Atu3871
Atu3872: Atu3872 - hypothetical protein, at 966,061 to 966,405
Atu3872
Atu3873: Atu3873 - hypothetical protein, at 966,601 to 967,581
Atu3873
Atu3874: Atu3874 - L-xylulose kinase, at 967,837 to 969,315
Atu3874
Position (kb) 966
967
968 Strain fitness (log2 ratio) -1
0
1
2 at 965.601 kb on + strand, within Atu3871 at 965.626 kb on + strand, within Atu3871 at 965.793 kb on + strand, within Atu3871 at 965.824 kb on + strand, within Atu3871 at 965.832 kb on - strand, within Atu3871 at 965.925 kb on - strand at 965.975 kb on + strand at 965.975 kb on - strand at 965.983 kb on - strand at 965.983 kb on - strand at 966.159 kb on - strand, within Atu3872 at 966.337 kb on + strand, within Atu3872 at 966.337 kb on + strand, within Atu3872 at 966.337 kb on + strand, within Atu3872 at 966.345 kb on - strand, within Atu3872 at 966.345 kb on - strand, within Atu3872 at 966.345 kb on - strand, within Atu3872 at 966.453 kb on + strand at 966.461 kb on - strand at 966.466 kb on + strand at 966.466 kb on + strand at 966.476 kb on + strand at 966.520 kb on + strand at 966.528 kb on - strand at 966.533 kb on - strand at 966.550 kb on - strand at 966.749 kb on + strand, within Atu3873 at 966.749 kb on + strand, within Atu3873 at 966.784 kb on + strand, within Atu3873 at 966.789 kb on - strand, within Atu3873 at 966.939 kb on - strand, within Atu3873 at 966.965 kb on + strand, within Atu3873 at 966.992 kb on + strand, within Atu3873 at 967.107 kb on + strand, within Atu3873 at 967.115 kb on - strand, within Atu3873 at 967.204 kb on + strand, within Atu3873 at 967.392 kb on + strand, within Atu3873 at 967.480 kb on - strand, within Atu3873 at 967.526 kb on + strand at 967.591 kb on + strand at 967.641 kb on + strand at 967.748 kb on + strand at 967.811 kb on + strand at 968.002 kb on - strand, within Atu3874 at 968.013 kb on + strand, within Atu3874 at 968.018 kb on + strand, within Atu3874 at 968.095 kb on + strand, within Atu3874 at 968.131 kb on + strand, within Atu3874 at 968.162 kb on + strand, within Atu3874 at 968.207 kb on + strand, within Atu3874 at 968.207 kb on + strand, within Atu3874 at 968.215 kb on - strand, within Atu3874 at 968.233 kb on + strand, within Atu3874 at 968.240 kb on - strand, within Atu3874 at 968.353 kb on - strand, within Atu3874 at 968.363 kb on - strand, within Atu3874 at 968.478 kb on - strand, within Atu3874 at 968.493 kb on + strand, within Atu3874 at 968.493 kb on + strand, within Atu3874 at 968.506 kb on - strand, within Atu3874 at 968.572 kb on + strand, within Atu3874 at 968.580 kb on + strand, within Atu3874
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Novobiocin 0.002 mM remove 965,601 + Atu3871 0.62 -0.0 965,626 + Atu3871 0.65 +0.6 965,793 + Atu3871 0.79 +0.8 965,824 + Atu3871 0.82 +0.3 965,832 - Atu3871 0.83 +1.2 965,925 - +1.4 965,975 + +0.7 965,975 - +0.8 965,983 - -0.5 965,983 - +0.3 966,159 - Atu3872 0.28 -0.1 966,337 + Atu3872 0.80 -0.6 966,337 + Atu3872 0.80 +0.0 966,337 + Atu3872 0.80 +0.5 966,345 - Atu3872 0.82 -0.4 966,345 - Atu3872 0.82 +0.4 966,345 - Atu3872 0.82 +0.0 966,453 + -1.1 966,461 - -0.8 966,466 + -0.7 966,466 + +0.1 966,476 + +0.4 966,520 + -0.4 966,528 - +0.5 966,533 - -0.3 966,550 - +0.2 966,749 + Atu3873 0.15 -0.7 966,749 + Atu3873 0.15 +0.7 966,784 + Atu3873 0.19 +0.9 966,789 - Atu3873 0.19 +0.6 966,939 - Atu3873 0.34 +1.5 966,965 + Atu3873 0.37 +0.5 966,992 + Atu3873 0.40 +0.1 967,107 + Atu3873 0.52 +1.0 967,115 - Atu3873 0.52 -0.1 967,204 + Atu3873 0.61 +1.8 967,392 + Atu3873 0.81 +0.6 967,480 - Atu3873 0.90 +0.5 967,526 + -0.2 967,591 + +0.4 967,641 + +1.0 967,748 + -0.6 967,811 + -0.6 968,002 - Atu3874 0.11 -0.7 968,013 + Atu3874 0.12 -0.8 968,018 + Atu3874 0.12 +0.7 968,095 + Atu3874 0.17 -1.6 968,131 + Atu3874 0.20 -0.5 968,162 + Atu3874 0.22 +1.4 968,207 + Atu3874 0.25 +0.7 968,207 + Atu3874 0.25 +0.3 968,215 - Atu3874 0.26 +2.1 968,233 + Atu3874 0.27 -0.2 968,240 - Atu3874 0.27 -0.3 968,353 - Atu3874 0.35 +0.6 968,363 - Atu3874 0.36 +0.6 968,478 - Atu3874 0.43 -0.9 968,493 + Atu3874 0.44 -0.0 968,493 + Atu3874 0.44 +0.7 968,506 - Atu3874 0.45 +0.2 968,572 + Atu3874 0.50 -0.5 968,580 + Atu3874 0.50 +1.0
Or see this region's nucleotide sequence