Experiment: R2A with Novobiocin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0222 and Atu0223 are separated by 133 nucleotides Atu0223 and Atu0224 are separated by 101 nucleotides Atu0224 and Atu0225 are separated by 70 nucleotides Atu0225 and Atu0226 are separated by 118 nucleotides
Atu0222: Atu0222 - components of type IV pilus, at 228,073 to 228,879
Atu0222
Atu0223: Atu0223 - components of type IV pilus, prepilin peptidase, at 229,013 to 229,525
Atu0223
Atu0224: Atu0224 - components of type IV pilus, pilin subunit, at 229,627 to 229,818
Atu0224
Atu0225: Atu0225 - hypothetical protein, at 229,889 to 230,143
Atu0225
Atu0226: Atu0226 - PilQ, at 230,262 to 230,645
Atu0226
Position (kb) 229
230 Strain fitness (log2 ratio) -1
0
1 at 228.661 kb on - strand, within Atu0222 at 228.922 kb on + strand at 229.035 kb on + strand at 229.048 kb on - strand at 229.070 kb on - strand, within Atu0223 at 229.121 kb on - strand, within Atu0223 at 229.190 kb on + strand, within Atu0223 at 229.218 kb on - strand, within Atu0223 at 229.222 kb on + strand, within Atu0223 at 229.272 kb on + strand, within Atu0223 at 229.272 kb on + strand, within Atu0223 at 229.280 kb on - strand, within Atu0223 at 229.280 kb on - strand, within Atu0223 at 229.431 kb on + strand, within Atu0223 at 229.446 kb on - strand, within Atu0223 at 229.466 kb on + strand, within Atu0223 at 229.487 kb on - strand at 229.545 kb on + strand at 229.553 kb on - strand at 229.580 kb on + strand at 229.606 kb on - strand at 229.606 kb on - strand at 229.611 kb on - strand at 229.640 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.646 kb on + strand at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.654 kb on - strand, within Atu0224 at 229.687 kb on + strand, within Atu0224 at 229.696 kb on - strand, within Atu0224 at 229.718 kb on + strand, within Atu0224 at 229.738 kb on + strand, within Atu0224 at 229.744 kb on - strand, within Atu0224 at 229.857 kb on + strand at 229.902 kb on + strand at 230.082 kb on + strand, within Atu0225 at 230.166 kb on - strand at 230.181 kb on - strand at 230.202 kb on + strand at 230.205 kb on - strand at 230.205 kb on - strand at 230.290 kb on - strand at 230.290 kb on - strand at 230.295 kb on - strand at 230.388 kb on - strand, within Atu0226 at 230.463 kb on - strand, within Atu0226 at 230.470 kb on + strand, within Atu0226 at 230.470 kb on + strand, within Atu0226 at 230.478 kb on - strand, within Atu0226 at 230.537 kb on + strand, within Atu0226 at 230.537 kb on + strand, within Atu0226 at 230.537 kb on + strand, within Atu0226 at 230.537 kb on + strand, within Atu0226 at 230.545 kb on - strand, within Atu0226 at 230.545 kb on - strand, within Atu0226 at 230.545 kb on - strand, within Atu0226 at 230.545 kb on - strand, within Atu0226 at 230.547 kb on - strand, within Atu0226 at 230.577 kb on + strand, within Atu0226 at 230.577 kb on + strand, within Atu0226 at 230.577 kb on + strand, within Atu0226 at 230.577 kb on + strand, within Atu0226 at 230.578 kb on - strand, within Atu0226 at 230.585 kb on - strand, within Atu0226 at 230.585 kb on - strand, within Atu0226 at 230.585 kb on - strand, within Atu0226 at 230.585 kb on - strand, within Atu0226 at 230.590 kb on - strand, within Atu0226 at 230.592 kb on - strand, within Atu0226 at 230.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Novobiocin 0.002 mM remove 228,661 - Atu0222 0.73 -0.1 228,922 + -0.2 229,035 + -0.0 229,048 - +0.1 229,070 - Atu0223 0.11 +0.2 229,121 - Atu0223 0.21 -0.5 229,190 + Atu0223 0.35 -0.0 229,218 - Atu0223 0.40 -0.3 229,222 + Atu0223 0.41 +0.4 229,272 + Atu0223 0.50 +0.2 229,272 + Atu0223 0.50 +0.1 229,280 - Atu0223 0.52 -0.1 229,280 - Atu0223 0.52 -0.2 229,431 + Atu0223 0.81 +0.2 229,446 - Atu0223 0.84 -0.5 229,466 + Atu0223 0.88 -0.1 229,487 - +0.1 229,545 + +1.4 229,553 - -0.2 229,580 + +0.1 229,606 - -1.1 229,606 - -0.3 229,611 - +0.2 229,640 + +0.3 229,646 + +0.2 229,646 + +1.1 229,646 + +0.1 229,646 + -0.9 229,646 + -0.1 229,646 + +0.4 229,646 + +0.1 229,646 + -1.6 229,646 + -0.9 229,646 + +0.1 229,646 + +1.0 229,646 + +0.2 229,654 - Atu0224 0.14 +0.1 229,654 - Atu0224 0.14 -0.9 229,654 - Atu0224 0.14 -0.1 229,654 - Atu0224 0.14 -0.2 229,654 - Atu0224 0.14 -0.2 229,654 - Atu0224 0.14 +0.3 229,654 - Atu0224 0.14 -0.1 229,654 - Atu0224 0.14 +0.6 229,687 + Atu0224 0.31 +0.8 229,696 - Atu0224 0.36 +1.2 229,718 + Atu0224 0.47 +0.3 229,738 + Atu0224 0.58 +1.3 229,744 - Atu0224 0.61 +1.0 229,857 + +0.3 229,902 + +0.5 230,082 + Atu0225 0.76 +0.6 230,166 - +0.2 230,181 - -0.3 230,202 + -0.9 230,205 - +0.3 230,205 - +0.5 230,290 - -0.4 230,290 - +0.3 230,295 - +0.2 230,388 - Atu0226 0.33 -0.2 230,463 - Atu0226 0.52 +0.4 230,470 + Atu0226 0.54 -0.8 230,470 + Atu0226 0.54 -0.5 230,478 - Atu0226 0.56 +0.2 230,537 + Atu0226 0.72 -0.2 230,537 + Atu0226 0.72 +0.1 230,537 + Atu0226 0.72 -1.5 230,537 + Atu0226 0.72 -0.7 230,545 - Atu0226 0.74 -0.1 230,545 - Atu0226 0.74 -0.2 230,545 - Atu0226 0.74 -0.7 230,545 - Atu0226 0.74 -0.6 230,547 - Atu0226 0.74 -0.3 230,577 + Atu0226 0.82 +0.8 230,577 + Atu0226 0.82 -1.0 230,577 + Atu0226 0.82 -0.1 230,577 + Atu0226 0.82 -0.2 230,578 - Atu0226 0.82 -0.4 230,585 - Atu0226 0.84 +0.1 230,585 - Atu0226 0.84 -0.6 230,585 - Atu0226 0.84 +0.4 230,585 - Atu0226 0.84 -0.8 230,590 - Atu0226 0.85 -0.5 230,592 - Atu0226 0.86 +1.2 230,639 + -0.4
Or see this region's nucleotide sequence