Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05185 and PS417_05190 are separated by 150 nucleotides PS417_05190 and PS417_05195 overlap by 8 nucleotides PS417_05195 and PS417_05200 are separated by 154 nucleotides PS417_05200 and PS417_05205 are separated by 51 nucleotides
PS417_05185: PS417_05185 - signal peptidase, at 1,151,593 to 1,152,447
_05185
PS417_05190: PS417_05190 - ribonuclease III, at 1,152,598 to 1,153,287
_05190
PS417_05195: PS417_05195 - GTPase Era, at 1,153,280 to 1,154,182
_05195
PS417_05200: PS417_05200 - DNA recombination protein RecO, at 1,154,337 to 1,155,026
_05200
PS417_05205: PS417_05205 - pyridoxine 5'-phosphate synthase, at 1,155,078 to 1,155,821
_05205
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-1
0
1 at 1152.445 kb on + strand at 1154.247 kb on + strand at 1154.248 kb on - strand at 1154.248 kb on - strand at 1154.313 kb on - strand at 1154.370 kb on + strand at 1154.371 kb on - strand at 1154.449 kb on + strand, within PS417_05200 at 1154.450 kb on - strand, within PS417_05200 at 1154.450 kb on - strand at 1154.579 kb on + strand, within PS417_05200 at 1154.608 kb on - strand, within PS417_05200 at 1154.608 kb on - strand, within PS417_05200 at 1154.820 kb on + strand, within PS417_05200 at 1155.037 kb on + strand at 1155.037 kb on + strand at 1155.038 kb on - strand at 1155.038 kb on - strand at 1155.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 1,152,445 + +0.1 1,154,247 + +0.1 1,154,248 - -0.2 1,154,248 - -0.2 1,154,313 - -0.2 1,154,370 + +0.5 1,154,371 - -1.3 1,154,449 + PS417_05200 0.16 +0.7 1,154,450 - PS417_05200 0.16 +0.6 1,154,450 - +0.7 1,154,579 + PS417_05200 0.35 -0.4 1,154,608 - PS417_05200 0.39 -0.0 1,154,608 - PS417_05200 0.39 -0.9 1,154,820 + PS417_05200 0.70 +0.7 1,155,037 + +0.3 1,155,037 + -0.5 1,155,038 - -0.5 1,155,038 - -0.2 1,155,038 - +0.4
Or see this region's nucleotide sequence