Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF604 and GFF605 are separated by 85 nucleotides GFF605 and GFF606 are separated by 35 nucleotides GFF606 and GFF607 are separated by 69 nucleotides GFF607 and GFF608 are separated by 51 nucleotides GFF608 and GFF609 are separated by 12 nucleotides
GFF604 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 343,700 to 344,383
GFF604
GFF605 - Biotin synthesis protein BioH, at 344,469 to 345,191
GFF605
GFF606 - Uncharacterized protein YfaD, at 345,227 to 346,141
GFF606
GFF607 - hypothetical protein, at 346,211 to 346,324
GFF607
GFF608 - Ferrous iron-sensisng transcriptional regulator FeoC, at 346,376 to 346,612
GFF608
GFF609 - Ferrous iron transport protein B, at 346,625 to 348,943
GFF609
Position (kb) 345
346
347 Strain fitness (log2 ratio) -2
-1
0
1 at 344.333 kb on + strand at 344.333 kb on + strand at 344.363 kb on + strand at 344.379 kb on - strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.384 kb on - strand at 344.384 kb on - strand at 344.415 kb on + strand at 344.415 kb on + strand at 344.415 kb on + strand at 344.416 kb on - strand at 344.446 kb on - strand at 344.504 kb on - strand at 344.540 kb on + strand at 344.938 kb on + strand, within GFF605 at 344.939 kb on - strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.106 kb on + strand, within GFF605 at 345.134 kb on + strand at 345.274 kb on + strand at 345.274 kb on + strand at 345.374 kb on - strand, within GFF606 at 345.392 kb on + strand, within GFF606 at 345.392 kb on + strand, within GFF606 at 345.406 kb on + strand, within GFF606 at 345.436 kb on + strand, within GFF606 at 345.437 kb on - strand, within GFF606 at 345.463 kb on + strand, within GFF606 at 345.498 kb on + strand, within GFF606 at 345.694 kb on - strand, within GFF606 at 345.696 kb on - strand, within GFF606 at 345.701 kb on - strand, within GFF606 at 345.808 kb on + strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.886 kb on + strand, within GFF606 at 345.909 kb on + strand, within GFF606 at 345.915 kb on + strand, within GFF606 at 346.240 kb on + strand, within GFF607 at 346.324 kb on + strand at 346.324 kb on + strand at 346.356 kb on - strand at 346.356 kb on - strand at 346.412 kb on - strand, within GFF608 at 346.468 kb on + strand, within GFF608 at 346.541 kb on - strand, within GFF608 at 346.617 kb on + strand at 346.626 kb on + strand at 346.728 kb on - strand at 346.776 kb on + strand at 346.776 kb on + strand at 346.831 kb on - strand at 346.913 kb on - strand, within GFF609 at 346.920 kb on - strand, within GFF609 at 346.923 kb on + strand, within GFF609 at 346.950 kb on + strand, within GFF609 at 347.023 kb on + strand, within GFF609 at 347.023 kb on + strand, within GFF609 at 347.059 kb on + strand, within GFF609 at 347.060 kb on - strand, within GFF609
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 344,333 + +0.2 344,333 + +1.0 344,363 + -0.1 344,379 - +1.6 344,383 + -0.2 344,383 + +0.3 344,383 + -0.1 344,383 + -0.3 344,383 + +0.3 344,383 + -0.0 344,383 + +0.5 344,384 - +0.2 344,384 - +0.1 344,415 + -0.1 344,415 + +0.5 344,415 + +0.2 344,416 - +0.2 344,446 - -0.1 344,504 - +1.6 344,540 + -0.0 344,938 + GFF605 0.65 +0.5 344,939 - GFF605 0.65 +0.6 345,027 + GFF605 0.77 -2.0 345,027 + GFF605 0.77 +0.8 345,027 + GFF605 0.77 +0.1 345,027 + GFF605 0.77 +0.1 345,028 - GFF605 0.77 +0.3 345,028 - GFF605 0.77 +0.2 345,028 - GFF605 0.77 -0.1 345,106 + GFF605 0.88 +0.7 345,134 + +0.6 345,274 + +0.7 345,274 + +0.4 345,374 - GFF606 0.16 +0.5 345,392 + GFF606 0.18 +0.6 345,392 + GFF606 0.18 +0.1 345,406 + GFF606 0.20 -0.1 345,436 + GFF606 0.23 -0.4 345,437 - GFF606 0.23 -1.0 345,463 + GFF606 0.26 +0.7 345,498 + GFF606 0.30 +0.4 345,694 - GFF606 0.51 +0.7 345,696 - GFF606 0.51 +0.6 345,701 - GFF606 0.52 +0.5 345,808 + GFF606 0.63 +0.6 345,872 - GFF606 0.70 +0.2 345,872 - GFF606 0.70 -0.3 345,886 + GFF606 0.72 +0.7 345,909 + GFF606 0.75 +1.0 345,915 + GFF606 0.75 -0.1 346,240 + GFF607 0.25 +0.4 346,324 + +0.3 346,324 + -1.6 346,356 - +0.2 346,356 - +0.8 346,412 - GFF608 0.15 +0.3 346,468 + GFF608 0.39 +0.2 346,541 - GFF608 0.70 +0.3 346,617 + +0.6 346,626 + +0.5 346,728 - +0.4 346,776 + +1.0 346,776 + +0.4 346,831 - +0.4 346,913 - GFF609 0.12 +1.1 346,920 - GFF609 0.13 +0.5 346,923 + GFF609 0.13 +0.6 346,950 + GFF609 0.14 +0.4 347,023 + GFF609 0.17 +0.7 347,023 + GFF609 0.17 +1.2 347,059 + GFF609 0.19 +0.7 347,060 - GFF609 0.19 +0.2
Or see this region's nucleotide sequence