Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF556 and GFF557 overlap by 4 nucleotides GFF557 and GFF558 are separated by 1 nucleotides GFF558 and GFF559 overlap by 4 nucleotides GFF559 and GFF560 are separated by 12 nucleotides
GFF556 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 286,781 to 287,668
GFF556
GFF557 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 287,665 to 288,510
GFF557
GFF558 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 288,512 to 289,582
GFF558
GFF559 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 289,579 to 290,319
GFF559
GFF560 - FIG00638953: hypothetical protein, at 290,332 to 290,748
GFF560
Position (kb)
288
289
290 Strain fitness (log2 ratio)
-1
0
1
2 at 287.651 kb on - strand at 287.869 kb on - strand, within GFF557 at 287.949 kb on + strand, within GFF557 at 287.949 kb on + strand, within GFF557 at 287.950 kb on - strand, within GFF557 at 288.097 kb on + strand, within GFF557 at 288.098 kb on - strand, within GFF557 at 288.293 kb on - strand, within GFF557 at 288.307 kb on + strand, within GFF557 at 288.307 kb on + strand, within GFF557 at 288.452 kb on + strand at 288.462 kb on - strand at 288.528 kb on + strand at 288.537 kb on + strand at 288.608 kb on - strand at 288.754 kb on - strand, within GFF558 at 288.764 kb on + strand, within GFF558 at 288.764 kb on + strand, within GFF558 at 288.764 kb on + strand, within GFF558 at 288.765 kb on - strand, within GFF558 at 288.789 kb on + strand, within GFF558 at 288.827 kb on - strand, within GFF558 at 288.827 kb on - strand, within GFF558 at 289.041 kb on + strand, within GFF558 at 289.079 kb on + strand, within GFF558 at 289.084 kb on + strand, within GFF558 at 289.085 kb on - strand, within GFF558 at 289.099 kb on - strand, within GFF558 at 289.138 kb on + strand, within GFF558 at 289.139 kb on - strand, within GFF558 at 289.139 kb on - strand, within GFF558 at 289.176 kb on + strand, within GFF558 at 289.179 kb on - strand, within GFF558 at 289.334 kb on - strand, within GFF558 at 289.334 kb on - strand, within GFF558 at 289.363 kb on + strand, within GFF558 at 289.364 kb on - strand, within GFF558 at 289.364 kb on - strand, within GFF558 at 289.407 kb on + strand, within GFF558 at 289.408 kb on - strand, within GFF558 at 289.411 kb on + strand, within GFF558 at 289.412 kb on - strand, within GFF558 at 289.412 kb on - strand, within GFF558 at 289.514 kb on - strand at 289.514 kb on - strand at 289.579 kb on - strand at 289.584 kb on + strand at 289.914 kb on + strand, within GFF559 at 290.027 kb on + strand, within GFF559 at 290.271 kb on + strand at 290.318 kb on - strand at 290.520 kb on + strand, within GFF560
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 287,651 - -0.2 287,869 - GFF557 0.24 -0.2 287,949 + GFF557 0.34 +0.3 287,949 + GFF557 0.34 -0.1 287,950 - GFF557 0.34 +0.2 288,097 + GFF557 0.51 +1.6 288,098 - GFF557 0.51 +0.1 288,293 - GFF557 0.74 +0.2 288,307 + GFF557 0.76 -0.4 288,307 + GFF557 0.76 -0.3 288,452 + +0.2 288,462 - -0.4 288,528 + +2.0 288,537 + +0.1 288,608 - -0.4 288,754 - GFF558 0.23 +0.3 288,764 + GFF558 0.24 +0.4 288,764 + GFF558 0.24 +0.6 288,764 + GFF558 0.24 +0.1 288,765 - GFF558 0.24 +0.7 288,789 + GFF558 0.26 +0.3 288,827 - GFF558 0.29 +0.3 288,827 - GFF558 0.29 +0.1 289,041 + GFF558 0.49 +0.8 289,079 + GFF558 0.53 -0.3 289,084 + GFF558 0.53 +1.9 289,085 - GFF558 0.54 -0.7 289,099 - GFF558 0.55 +0.5 289,138 + GFF558 0.58 +0.0 289,139 - GFF558 0.59 -0.2 289,139 - GFF558 0.59 -0.5 289,176 + GFF558 0.62 +0.5 289,179 - GFF558 0.62 +0.3 289,334 - GFF558 0.77 +0.9 289,334 - GFF558 0.77 -0.6 289,363 + GFF558 0.79 -0.8 289,364 - GFF558 0.80 +0.3 289,364 - GFF558 0.80 -0.0 289,407 + GFF558 0.84 +1.1 289,408 - GFF558 0.84 -0.4 289,411 + GFF558 0.84 +0.0 289,412 - GFF558 0.84 +0.4 289,412 - GFF558 0.84 -0.3 289,514 - +0.3 289,514 - -0.6 289,579 - -0.1 289,584 + -0.2 289,914 + GFF559 0.45 +1.2 290,027 + GFF559 0.60 +0.6 290,271 + +0.2 290,318 - +0.7 290,520 + GFF560 0.45 -0.9
Or see this region's nucleotide sequence