Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF555 and GFF556 are separated by 63 nucleotides GFF556 and GFF557 overlap by 4 nucleotides GFF557 and GFF558 are separated by 1 nucleotides
GFF555 - Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3), at 285,401 to 286,717
GFF555
GFF556 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 286,781 to 287,668
GFF556
GFF557 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 287,665 to 288,510
GFF557
GFF558 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 288,512 to 289,582
GFF558
Position (kb) 286
287
288 Strain fitness (log2 ratio) -1
0
1
2 at 285.783 kb on - strand, within GFF555 at 285.783 kb on - strand, within GFF555 at 285.824 kb on - strand, within GFF555 at 285.849 kb on - strand, within GFF555 at 285.923 kb on + strand, within GFF555 at 285.923 kb on + strand, within GFF555 at 285.924 kb on - strand, within GFF555 at 286.142 kb on + strand, within GFF555 at 286.253 kb on + strand, within GFF555 at 286.254 kb on - strand, within GFF555 at 286.330 kb on - strand, within GFF555 at 286.485 kb on - strand, within GFF555 at 286.534 kb on + strand, within GFF555 at 286.576 kb on + strand, within GFF555 at 286.576 kb on + strand, within GFF555 at 286.721 kb on + strand at 286.721 kb on + strand at 286.722 kb on - strand at 286.722 kb on - strand at 286.723 kb on + strand at 286.723 kb on + strand at 286.724 kb on - strand at 286.979 kb on + strand, within GFF556 at 286.980 kb on - strand, within GFF556 at 287.075 kb on + strand, within GFF556 at 287.075 kb on + strand, within GFF556 at 287.076 kb on - strand, within GFF556 at 287.123 kb on + strand, within GFF556 at 287.151 kb on + strand, within GFF556 at 287.188 kb on - strand, within GFF556 at 287.265 kb on + strand, within GFF556 at 287.290 kb on + strand, within GFF556 at 287.291 kb on - strand, within GFF556 at 287.411 kb on - strand, within GFF556 at 287.651 kb on - strand at 287.869 kb on - strand, within GFF557 at 287.949 kb on + strand, within GFF557 at 287.949 kb on + strand, within GFF557 at 287.950 kb on - strand, within GFF557 at 288.097 kb on + strand, within GFF557 at 288.098 kb on - strand, within GFF557 at 288.293 kb on - strand, within GFF557 at 288.307 kb on + strand, within GFF557 at 288.307 kb on + strand, within GFF557 at 288.452 kb on + strand at 288.462 kb on - strand at 288.528 kb on + strand at 288.537 kb on + strand at 288.608 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 285,783 - GFF555 0.29 +1.0 285,783 - GFF555 0.29 +0.7 285,824 - GFF555 0.32 +0.1 285,849 - GFF555 0.34 +0.5 285,923 + GFF555 0.40 -0.1 285,923 + GFF555 0.40 +0.6 285,924 - GFF555 0.40 +0.2 286,142 + GFF555 0.56 +1.6 286,253 + GFF555 0.65 -0.2 286,254 - GFF555 0.65 -1.2 286,330 - GFF555 0.71 +0.9 286,485 - GFF555 0.82 +0.6 286,534 + GFF555 0.86 +0.7 286,576 + GFF555 0.89 +0.2 286,576 + GFF555 0.89 +0.5 286,721 + +0.4 286,721 + +0.2 286,722 - +0.1 286,722 - +0.2 286,723 + +0.1 286,723 + +0.2 286,724 - +0.2 286,979 + GFF556 0.22 -0.0 286,980 - GFF556 0.22 -0.0 287,075 + GFF556 0.33 -0.3 287,075 + GFF556 0.33 -0.2 287,076 - GFF556 0.33 -0.4 287,123 + GFF556 0.39 -0.2 287,151 + GFF556 0.42 +0.1 287,188 - GFF556 0.46 +0.5 287,265 + GFF556 0.55 +0.6 287,290 + GFF556 0.57 -0.0 287,291 - GFF556 0.57 -0.1 287,411 - GFF556 0.71 +0.1 287,651 - -0.2 287,869 - GFF557 0.24 -0.2 287,949 + GFF557 0.34 +0.3 287,949 + GFF557 0.34 -0.1 287,950 - GFF557 0.34 +0.2 288,097 + GFF557 0.51 +1.6 288,098 - GFF557 0.51 +0.1 288,293 - GFF557 0.74 +0.2 288,307 + GFF557 0.76 -0.4 288,307 + GFF557 0.76 -0.3 288,452 + +0.2 288,462 - -0.4 288,528 + +2.0 288,537 + +0.1 288,608 - -0.4
Or see this region's nucleotide sequence