Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4346 and GFF4347 are separated by 273 nucleotides GFF4347 and GFF4348 are separated by 83 nucleotides GFF4348 and GFF4349 are separated by 43 nucleotides GFF4349 and GFF4350 are separated by 7 nucleotides GFF4350 and GFF4351 are separated by 122 nucleotides
GFF4346 - Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-), at 127,403 to 128,332
GFF4346
GFF4347 - hypothetical protein, at 128,606 to 128,728
GFF4347
GFF4348 - AmpG permease, at 128,812 to 130,287
GFF4348
GFF4349 - Hypothetical lipoprotein YajG precursor, at 130,331 to 130,963
GFF4349
GFF4350 - hypothetical protein, at 130,971 to 131,090
GFF4350
GFF4351 - Cell division protein BolA, at 131,213 to 131,530
GFF4351
Position (kb) 128
129
130
131 Strain fitness (log2 ratio) -2
-1
0
1 at 127.936 kb on - strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.143 kb on + strand, within GFF4346 at 128.316 kb on - strand at 128.338 kb on + strand at 128.429 kb on + strand at 128.481 kb on + strand at 128.493 kb on + strand at 128.493 kb on + strand at 128.509 kb on + strand at 128.774 kb on + strand at 128.813 kb on + strand at 128.925 kb on + strand at 128.930 kb on + strand at 129.064 kb on - strand, within GFF4348 at 129.064 kb on - strand, within GFF4348 at 129.064 kb on - strand, within GFF4348 at 129.065 kb on + strand, within GFF4348 at 129.066 kb on - strand, within GFF4348 at 129.066 kb on - strand, within GFF4348 at 129.250 kb on - strand, within GFF4348 at 129.250 kb on - strand, within GFF4348 at 129.253 kb on - strand, within GFF4348 at 129.253 kb on - strand, within GFF4348 at 129.624 kb on + strand, within GFF4348 at 129.758 kb on + strand, within GFF4348 at 129.759 kb on - strand, within GFF4348 at 130.057 kb on + strand, within GFF4348 at 130.105 kb on - strand, within GFF4348 at 130.112 kb on + strand, within GFF4348 at 130.113 kb on - strand, within GFF4348 at 130.183 kb on + strand at 130.274 kb on + strand at 130.287 kb on + strand at 130.288 kb on - strand at 130.288 kb on - strand at 130.296 kb on - strand at 130.330 kb on - strand at 130.381 kb on + strand at 130.382 kb on - strand at 130.382 kb on - strand at 130.435 kb on + strand, within GFF4349 at 130.465 kb on - strand, within GFF4349 at 130.465 kb on - strand, within GFF4349 at 130.579 kb on - strand, within GFF4349 at 130.596 kb on - strand, within GFF4349 at 130.596 kb on - strand, within GFF4349 at 130.631 kb on - strand, within GFF4349 at 130.632 kb on + strand, within GFF4349 at 130.633 kb on - strand, within GFF4349 at 130.633 kb on - strand, within GFF4349 at 130.670 kb on + strand, within GFF4349 at 130.671 kb on - strand, within GFF4349 at 131.013 kb on - strand, within GFF4350 at 131.166 kb on + strand at 131.167 kb on - strand at 131.220 kb on + strand at 131.220 kb on + strand at 131.221 kb on - strand at 131.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 127,936 - GFF4346 0.57 -1.4 128,127 + GFF4346 0.78 -1.8 128,127 + GFF4346 0.78 -1.4 128,143 + GFF4346 0.80 -0.3 128,316 - -1.2 128,338 + -0.9 128,429 + -1.3 128,481 + +0.2 128,493 + +0.5 128,493 + +0.0 128,509 + +0.1 128,774 + +0.6 128,813 + +1.2 128,925 + +0.1 128,930 + -0.2 129,064 - GFF4348 0.17 -0.3 129,064 - GFF4348 0.17 -0.1 129,064 - GFF4348 0.17 +0.4 129,065 + GFF4348 0.17 +0.6 129,066 - GFF4348 0.17 -0.0 129,066 - GFF4348 0.17 -1.3 129,250 - GFF4348 0.30 -0.2 129,250 - GFF4348 0.30 +0.0 129,253 - GFF4348 0.30 +0.2 129,253 - GFF4348 0.30 +0.2 129,624 + GFF4348 0.55 +0.4 129,758 + GFF4348 0.64 +0.6 129,759 - GFF4348 0.64 -0.0 130,057 + GFF4348 0.84 +0.3 130,105 - GFF4348 0.88 +0.3 130,112 + GFF4348 0.88 -0.3 130,113 - GFF4348 0.88 -0.1 130,183 + +0.7 130,274 + +1.0 130,287 + +0.6 130,288 - +0.1 130,288 - +0.8 130,296 - +0.4 130,330 - +0.2 130,381 + +0.3 130,382 - +0.4 130,382 - +0.4 130,435 + GFF4349 0.16 +1.1 130,465 - GFF4349 0.21 +0.3 130,465 - GFF4349 0.21 +0.1 130,579 - GFF4349 0.39 +0.1 130,596 - GFF4349 0.42 +0.4 130,596 - GFF4349 0.42 -0.0 130,631 - GFF4349 0.47 +0.3 130,632 + GFF4349 0.48 -0.1 130,633 - GFF4349 0.48 +0.5 130,633 - GFF4349 0.48 +0.1 130,670 + GFF4349 0.54 +0.7 130,671 - GFF4349 0.54 +0.3 131,013 - GFF4350 0.35 +0.7 131,166 + +0.2 131,167 - -0.2 131,220 + +0.0 131,220 + -1.5 131,221 - +0.3 131,233 - +0.4
Or see this region's nucleotide sequence