Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3768 and GFF3769 are separated by 9 nucleotides GFF3769 and GFF3770 are separated by 84 nucleotides GFF3770 and GFF3771 are separated by 20 nucleotides GFF3771 and GFF3772 overlap by 1 nucleotides GFF3772 and GFF3773 are separated by 16 nucleotides
GFF3768 - Sugar efflux transporter B, at 35,947 to 37,128
GFF3768
GFF3769 - hypothetical protein, at 37,138 to 37,260
GFF3769
GFF3770 - hypothetical protein, at 37,345 to 37,476
GFF3770
GFF3771 - Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69), at 37,497 to 38,627
GFF3771
GFF3772 - 1-phosphofructokinase (EC 2.7.1.56), at 38,627 to 39,565
GFF3772
GFF3773 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 39,582 to 41,270
GFF3773
Position (kb) 37
38
39 Strain fitness (log2 ratio) -1
0
1 at 36.603 kb on - strand, within GFF3768 at 36.618 kb on + strand, within GFF3768 at 36.815 kb on + strand, within GFF3768 at 36.815 kb on + strand, within GFF3768 at 37.098 kb on + strand at 37.134 kb on + strand at 37.241 kb on - strand, within GFF3769 at 37.298 kb on + strand at 37.622 kb on + strand, within GFF3771 at 37.777 kb on - strand, within GFF3771 at 37.790 kb on + strand, within GFF3771 at 38.173 kb on + strand, within GFF3771 at 38.194 kb on + strand, within GFF3771 at 38.351 kb on - strand, within GFF3771 at 38.666 kb on + strand at 39.118 kb on + strand, within GFF3772 at 39.370 kb on - strand, within GFF3772 at 39.446 kb on - strand, within GFF3772 at 39.549 kb on + strand at 39.598 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 36,603 - GFF3768 0.55 +0.4 36,618 + GFF3768 0.57 -0.0 36,815 + GFF3768 0.73 +0.0 36,815 + GFF3768 0.73 -0.0 37,098 + +0.2 37,134 + +0.7 37,241 - GFF3769 0.84 -0.1 37,298 + +0.2 37,622 + GFF3771 0.11 -0.6 37,777 - GFF3771 0.25 -0.3 37,790 + GFF3771 0.26 -0.1 38,173 + GFF3771 0.60 -0.4 38,194 + GFF3771 0.62 +0.5 38,351 - GFF3771 0.76 -0.5 38,666 + -0.8 39,118 + GFF3772 0.52 -1.0 39,370 - GFF3772 0.79 -1.5 39,446 - GFF3772 0.87 -0.8 39,549 + +0.5 39,598 + -0.3
Or see this region's nucleotide sequence