Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2882 and GFF2883 are separated by 4 nucleotides GFF2883 and GFF2884 overlap by 23 nucleotides GFF2884 and GFF2885 are separated by 32 nucleotides GFF2885 and GFF2886 overlap by 1 nucleotides
GFF2882 - Lipopolysaccharide biosynthesis protein RffA, at 6,397 to 7,527
GFF2882
GFF2883 - Lipopolysaccharide biosynthesis protein RffC, at 7,532 to 8,209
GFF2883
GFF2884 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 8,187 to 8,411
GFF2884
GFF2885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 8,444 to 9,511
GFF2885
GFF2886 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 9,511 to 10,773
GFF2886
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 7.481 kb on + strand at 7.481 kb on + strand at 7.481 kb on + strand at 7.539 kb on + strand at 7.540 kb on - strand at 7.580 kb on + strand at 7.581 kb on - strand at 7.584 kb on + strand at 7.980 kb on - strand, within GFF2883 at 8.168 kb on - strand at 8.180 kb on + strand at 8.237 kb on - strand, within GFF2884 at 8.350 kb on - strand, within GFF2884 at 8.350 kb on - strand, within GFF2884 at 8.360 kb on + strand, within GFF2884 at 8.431 kb on - strand at 8.615 kb on + strand, within GFF2885 at 8.615 kb on + strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.648 kb on - strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.802 kb on + strand, within GFF2885 at 8.803 kb on - strand, within GFF2885 at 8.862 kb on + strand, within GFF2885 at 8.931 kb on + strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.947 kb on + strand, within GFF2885 at 8.947 kb on + strand, within GFF2885 at 8.948 kb on - strand, within GFF2885 at 8.969 kb on - strand, within GFF2885 at 8.979 kb on + strand, within GFF2885 at 8.984 kb on - strand, within GFF2885 at 9.052 kb on - strand, within GFF2885 at 9.118 kb on + strand, within GFF2885 at 9.118 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.196 kb on - strand, within GFF2885 at 9.251 kb on + strand, within GFF2885 at 9.252 kb on - strand, within GFF2885 at 9.729 kb on + strand, within GFF2886 at 9.954 kb on + strand, within GFF2886 at 10.226 kb on - strand, within GFF2886 at 10.485 kb on - strand, within GFF2886 at 10.485 kb on - strand, within GFF2886
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 7,481 + -1.1 7,481 + -0.9 7,481 + -0.2 7,539 + -0.8 7,540 - -2.1 7,580 + -0.2 7,581 - -2.8 7,584 + -0.4 7,980 - GFF2883 0.66 +0.2 8,168 - -0.5 8,180 + -1.6 8,237 - GFF2884 0.22 +0.0 8,350 - GFF2884 0.72 +0.3 8,350 - GFF2884 0.72 +0.4 8,360 + GFF2884 0.77 -0.8 8,431 - +0.5 8,615 + GFF2885 0.16 +0.1 8,615 + GFF2885 0.16 -0.6 8,616 - GFF2885 0.16 -1.3 8,616 - GFF2885 0.16 +0.2 8,616 - GFF2885 0.16 -0.2 8,616 - GFF2885 0.16 +0.5 8,616 - GFF2885 0.16 -0.2 8,616 - GFF2885 0.16 +0.3 8,648 - GFF2885 0.19 +0.1 8,766 + GFF2885 0.30 +0.4 8,766 + GFF2885 0.30 +0.7 8,766 + GFF2885 0.30 +0.2 8,766 + GFF2885 0.30 +0.1 8,766 + GFF2885 0.30 -0.4 8,767 - GFF2885 0.30 +1.2 8,767 - GFF2885 0.30 -0.0 8,767 - GFF2885 0.30 -0.6 8,802 + GFF2885 0.34 -0.1 8,803 - GFF2885 0.34 +0.1 8,862 + GFF2885 0.39 +0.6 8,931 + GFF2885 0.46 -1.3 8,945 - GFF2885 0.47 +0.3 8,945 - GFF2885 0.47 +0.2 8,945 - GFF2885 0.47 +0.2 8,947 + GFF2885 0.47 -1.4 8,947 + GFF2885 0.47 -0.7 8,948 - GFF2885 0.47 +0.1 8,969 - GFF2885 0.49 -0.0 8,979 + GFF2885 0.50 -0.1 8,984 - GFF2885 0.51 +0.5 9,052 - GFF2885 0.57 +0.1 9,118 + GFF2885 0.63 -1.4 9,118 + GFF2885 0.63 -0.4 9,195 + GFF2885 0.70 -0.8 9,195 + GFF2885 0.70 -0.3 9,195 + GFF2885 0.70 +0.2 9,195 + GFF2885 0.70 -0.8 9,196 - GFF2885 0.70 -0.1 9,251 + GFF2885 0.76 -0.4 9,252 - GFF2885 0.76 -0.7 9,729 + GFF2886 0.17 -1.2 9,954 + GFF2886 0.35 +0.5 10,226 - GFF2886 0.57 +0.2 10,485 - GFF2886 0.77 +0.0 10,485 - GFF2886 0.77 -0.2
Or see this region's nucleotide sequence