Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2063 and GFF2064 overlap by 14 nucleotides GFF2064 and GFF2065 are separated by 212 nucleotides GFF2065 and GFF2066 are separated by 2 nucleotides
GFF2063 - Inner membrane protein YfeZ, at 27,278 to 27,727
GFF2063
GFF2064 - Acetyltransferase YpeA, at 27,714 to 28,139
GFF2064
GFF2065 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 28,352 to 29,221
GFF2065
GFF2066 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 29,224 to 30,123
GFF2066
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 27.583 kb on + strand, within GFF2063 at 27.787 kb on + strand, within GFF2064 at 27.787 kb on + strand, within GFF2064 at 27.787 kb on + strand, within GFF2064 at 27.788 kb on - strand, within GFF2064 at 27.788 kb on - strand, within GFF2064 at 27.810 kb on - strand, within GFF2064 at 27.928 kb on + strand, within GFF2064 at 27.928 kb on + strand, within GFF2064 at 28.131 kb on + strand at 28.177 kb on - strand at 28.267 kb on + strand at 28.317 kb on - strand at 28.436 kb on - strand at 28.436 kb on - strand at 28.450 kb on - strand, within GFF2065 at 28.718 kb on - strand, within GFF2065 at 28.739 kb on - strand, within GFF2065 at 28.835 kb on + strand, within GFF2065 at 28.836 kb on - strand, within GFF2065 at 28.905 kb on + strand, within GFF2065 at 28.906 kb on - strand, within GFF2065 at 29.086 kb on + strand, within GFF2065 at 29.370 kb on - strand, within GFF2066 at 29.525 kb on - strand, within GFF2066 at 29.525 kb on - strand, within GFF2066 at 29.623 kb on + strand, within GFF2066 at 29.624 kb on - strand, within GFF2066 at 29.848 kb on + strand, within GFF2066 at 30.100 kb on - strand at 30.132 kb on + strand at 30.132 kb on + strand at 30.137 kb on + strand at 30.169 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 27,583 + GFF2063 0.68 -0.2 27,787 + GFF2064 0.17 +0.5 27,787 + GFF2064 0.17 -0.5 27,787 + GFF2064 0.17 +0.1 27,788 - GFF2064 0.17 +0.2 27,788 - GFF2064 0.17 +0.2 27,810 - GFF2064 0.23 +1.0 27,928 + GFF2064 0.50 +1.7 27,928 + GFF2064 0.50 -1.8 28,131 + -0.3 28,177 - -0.5 28,267 + -0.1 28,317 - -0.4 28,436 - -0.3 28,436 - -0.9 28,450 - GFF2065 0.11 -0.7 28,718 - GFF2065 0.42 +0.4 28,739 - GFF2065 0.44 -0.0 28,835 + GFF2065 0.56 -0.6 28,836 - GFF2065 0.56 -0.3 28,905 + GFF2065 0.64 -0.0 28,906 - GFF2065 0.64 -0.5 29,086 + GFF2065 0.84 -1.2 29,370 - GFF2066 0.16 -0.2 29,525 - GFF2066 0.33 +1.1 29,525 - GFF2066 0.33 -0.7 29,623 + GFF2066 0.44 -1.0 29,624 - GFF2066 0.44 +0.0 29,848 + GFF2066 0.69 +0.2 30,100 - -0.1 30,132 + -0.4 30,132 + +0.6 30,137 + +0.3 30,169 + +0.2
Or see this region's nucleotide sequence