Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1881 and GFF1882 are separated by 2 nucleotides GFF1882 and GFF1883 overlap by 4 nucleotides GFF1883 and GFF1884 are separated by 10 nucleotides GFF1884 and GFF1885 are separated by 14 nucleotides GFF1885 and GFF1886 overlap by 4 nucleotides
GFF1881 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 74,054 to 75,889
GFF1881
GFF1882 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 75,892 to 76,194
GFF1882
GFF1883 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 76,191 to 76,745
GFF1883
GFF1884 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 76,756 to 77,298
GFF1884
GFF1885 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 77,313 to 78,290
GFF1885
GFF1886 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 78,287 to 81,013
GFF1886
Position (kb) 76
77
78 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 75.872 kb on - strand at 75.911 kb on - strand at 76.010 kb on + strand, within GFF1882 at 76.010 kb on + strand, within GFF1882 at 76.010 kb on + strand, within GFF1882 at 76.011 kb on - strand, within GFF1882 at 76.011 kb on - strand, within GFF1882 at 76.105 kb on - strand, within GFF1882 at 76.153 kb on + strand, within GFF1882 at 76.310 kb on + strand, within GFF1883 at 76.311 kb on - strand, within GFF1883 at 76.311 kb on - strand, within GFF1883 at 76.336 kb on + strand, within GFF1883 at 76.421 kb on - strand, within GFF1883 at 76.600 kb on + strand, within GFF1883 at 76.600 kb on + strand, within GFF1883 at 76.601 kb on - strand, within GFF1883 at 76.655 kb on - strand, within GFF1883 at 76.679 kb on + strand, within GFF1883 at 76.728 kb on - strand at 76.757 kb on + strand at 76.857 kb on - strand, within GFF1884 at 77.157 kb on + strand, within GFF1884 at 77.400 kb on + strand at 77.401 kb on - strand at 77.411 kb on - strand, within GFF1885 at 77.566 kb on + strand, within GFF1885 at 77.766 kb on - strand, within GFF1885 at 77.769 kb on - strand, within GFF1885 at 77.824 kb on + strand, within GFF1885 at 77.870 kb on - strand, within GFF1885 at 77.975 kb on + strand, within GFF1885 at 78.152 kb on - strand, within GFF1885
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 75,872 - -2.7 75,911 - -2.1 76,010 + GFF1882 0.39 -3.1 76,010 + GFF1882 0.39 -4.3 76,010 + GFF1882 0.39 -2.8 76,011 - GFF1882 0.39 -4.0 76,011 - GFF1882 0.39 -0.7 76,105 - GFF1882 0.70 -3.3 76,153 + GFF1882 0.86 -3.9 76,310 + GFF1883 0.21 -4.2 76,311 - GFF1883 0.22 -3.9 76,311 - GFF1883 0.22 -2.7 76,336 + GFF1883 0.26 -2.3 76,421 - GFF1883 0.41 -3.5 76,600 + GFF1883 0.74 -3.5 76,600 + GFF1883 0.74 -2.6 76,601 - GFF1883 0.74 -3.1 76,655 - GFF1883 0.84 -4.0 76,679 + GFF1883 0.88 -5.1 76,728 - -5.2 76,757 + -3.5 76,857 - GFF1884 0.19 -3.1 77,157 + GFF1884 0.74 -2.1 77,400 + -4.0 77,401 - -1.4 77,411 - GFF1885 0.10 -4.3 77,566 + GFF1885 0.26 -3.9 77,766 - GFF1885 0.46 -4.1 77,769 - GFF1885 0.47 -3.3 77,824 + GFF1885 0.52 -2.3 77,870 - GFF1885 0.57 -2.5 77,975 + GFF1885 0.68 -3.6 78,152 - GFF1885 0.86 -3.0
Or see this region's nucleotide sequence