Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1879 and GFF1880 are separated by 6 nucleotides GFF1880 and GFF1881 are separated by 313 nucleotides
GFF1879 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 70,747 to 72,204
GFF1879
GFF1880 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 72,211 to 73,740
GFF1880
GFF1881 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 74,054 to 75,889
GFF1881
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 71.299 kb on - strand, within GFF1879 at 71.365 kb on - strand, within GFF1879 at 71.488 kb on - strand, within GFF1879 at 71.513 kb on + strand, within GFF1879 at 71.616 kb on + strand, within GFF1879 at 71.616 kb on + strand, within GFF1879 at 71.673 kb on - strand, within GFF1879 at 71.673 kb on - strand, within GFF1879 at 71.729 kb on + strand, within GFF1879 at 71.730 kb on - strand, within GFF1879 at 71.888 kb on + strand, within GFF1879 at 71.889 kb on - strand, within GFF1879 at 71.979 kb on - strand, within GFF1879 at 72.225 kb on + strand at 72.226 kb on - strand at 72.300 kb on - strand at 72.342 kb on + strand at 72.411 kb on - strand, within GFF1880 at 72.439 kb on + strand, within GFF1880 at 72.452 kb on - strand, within GFF1880 at 72.452 kb on - strand, within GFF1880 at 72.624 kb on + strand, within GFF1880 at 72.640 kb on - strand, within GFF1880 at 72.653 kb on + strand, within GFF1880 at 72.654 kb on - strand, within GFF1880 at 72.749 kb on + strand, within GFF1880 at 72.790 kb on - strand, within GFF1880 at 72.790 kb on - strand, within GFF1880 at 72.791 kb on + strand, within GFF1880 at 72.792 kb on - strand, within GFF1880 at 72.871 kb on - strand, within GFF1880 at 72.871 kb on - strand, within GFF1880 at 73.212 kb on - strand, within GFF1880 at 73.474 kb on + strand, within GFF1880 at 73.506 kb on + strand, within GFF1880 at 73.506 kb on + strand, within GFF1880 at 73.736 kb on - strand at 73.745 kb on - strand at 73.887 kb on + strand at 73.887 kb on + strand at 73.888 kb on - strand at 73.888 kb on - strand at 73.896 kb on - strand at 74.011 kb on + strand at 74.115 kb on + strand at 74.116 kb on - strand at 74.116 kb on - strand at 74.116 kb on - strand at 74.128 kb on - strand at 74.252 kb on + strand, within GFF1881 at 74.253 kb on - strand, within GFF1881 at 74.263 kb on - strand, within GFF1881 at 74.289 kb on + strand, within GFF1881 at 74.290 kb on - strand, within GFF1881
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 71,299 - GFF1879 0.38 -2.1 71,365 - GFF1879 0.42 -3.1 71,488 - GFF1879 0.51 -2.9 71,513 + GFF1879 0.53 -3.7 71,616 + GFF1879 0.60 -3.3 71,616 + GFF1879 0.60 -0.9 71,673 - GFF1879 0.64 -4.7 71,673 - GFF1879 0.64 -1.9 71,729 + GFF1879 0.67 -4.3 71,730 - GFF1879 0.67 -3.2 71,888 + GFF1879 0.78 -2.7 71,889 - GFF1879 0.78 -3.9 71,979 - GFF1879 0.84 -3.0 72,225 + -5.1 72,226 - -2.8 72,300 - -4.2 72,342 + -2.1 72,411 - GFF1880 0.13 -2.8 72,439 + GFF1880 0.15 -3.2 72,452 - GFF1880 0.16 -2.8 72,452 - GFF1880 0.16 -3.8 72,624 + GFF1880 0.27 -4.6 72,640 - GFF1880 0.28 -3.7 72,653 + GFF1880 0.29 -2.7 72,654 - GFF1880 0.29 -2.7 72,749 + GFF1880 0.35 -3.8 72,790 - GFF1880 0.38 -4.1 72,790 - GFF1880 0.38 -2.2 72,791 + GFF1880 0.38 -4.9 72,792 - GFF1880 0.38 -3.5 72,871 - GFF1880 0.43 -3.2 72,871 - GFF1880 0.43 -3.7 73,212 - GFF1880 0.65 -3.7 73,474 + GFF1880 0.83 -2.1 73,506 + GFF1880 0.85 -3.8 73,506 + GFF1880 0.85 -1.8 73,736 - -4.2 73,745 - -3.5 73,887 + -2.1 73,887 + -5.4 73,888 - -3.6 73,888 - -2.3 73,896 - -2.0 74,011 + -3.0 74,115 + -2.1 74,116 - -4.4 74,116 - -3.9 74,116 - -3.0 74,128 - -4.1 74,252 + GFF1881 0.11 -1.6 74,253 - GFF1881 0.11 -3.9 74,263 - GFF1881 0.11 -3.1 74,289 + GFF1881 0.13 -2.8 74,290 - GFF1881 0.13 -3.3
Or see this region's nucleotide sequence