Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF170 and GFF171 are separated by 13 nucleotides GFF171 and GFF172 are separated by 44 nucleotides GFF172 and GFF173 overlap by 25 nucleotides
GFF170 - SgrR, sugar-phosphate stress, transcriptional activator of SgrS small RNA, at 160,584 to 162,242
GFF170
GFF171 - hypothetical protein, at 162,256 to 162,375
GFF171
GFF172 - Thiamin ABC transporter, substrate-binding component, at 162,420 to 163,403
GFF172
GFF173 - Thiamin ABC transporter, transmembrane component, at 163,379 to 164,989
GFF173
Position (kb) 162
163
164 Strain fitness (log2 ratio) -1
0
1
2 at 161.470 kb on + strand, within GFF170 at 161.592 kb on + strand, within GFF170 at 161.595 kb on + strand, within GFF170 at 161.685 kb on + strand, within GFF170 at 161.704 kb on + strand, within GFF170 at 161.958 kb on - strand, within GFF170 at 162.007 kb on + strand, within GFF170 at 162.188 kb on + strand at 162.189 kb on - strand at 162.280 kb on - strand, within GFF171 at 162.284 kb on + strand, within GFF171 at 162.492 kb on + strand at 162.698 kb on + strand, within GFF172 at 163.140 kb on + strand, within GFF172 at 163.140 kb on + strand, within GFF172 at 163.141 kb on - strand, within GFF172 at 163.144 kb on + strand, within GFF172 at 163.144 kb on + strand, within GFF172 at 163.145 kb on - strand, within GFF172 at 163.150 kb on + strand, within GFF172 at 163.151 kb on - strand, within GFF172 at 163.151 kb on - strand, within GFF172 at 163.529 kb on + strand at 163.690 kb on - strand, within GFF173 at 163.721 kb on + strand, within GFF173 at 163.722 kb on - strand, within GFF173 at 164.090 kb on + strand, within GFF173 at 164.158 kb on - strand, within GFF173 at 164.256 kb on + strand, within GFF173 at 164.257 kb on - strand, within GFF173
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 161,470 + GFF170 0.53 -0.4 161,592 + GFF170 0.61 -0.1 161,595 + GFF170 0.61 +0.4 161,685 + GFF170 0.66 +2.7 161,704 + GFF170 0.68 +0.9 161,958 - GFF170 0.83 +0.1 162,007 + GFF170 0.86 +0.1 162,188 + -0.2 162,189 - -0.4 162,280 - GFF171 0.20 +0.7 162,284 + GFF171 0.23 -0.4 162,492 + +0.4 162,698 + GFF172 0.28 -0.4 163,140 + GFF172 0.73 +0.1 163,140 + GFF172 0.73 -0.8 163,141 - GFF172 0.73 -0.0 163,144 + GFF172 0.74 +1.1 163,144 + GFF172 0.74 +0.3 163,145 - GFF172 0.74 -1.0 163,150 + GFF172 0.74 -0.1 163,151 - GFF172 0.74 -0.8 163,151 - GFF172 0.74 -0.3 163,529 + -1.2 163,690 - GFF173 0.19 +2.0 163,721 + GFF173 0.21 +0.5 163,722 - GFF173 0.21 +0.1 164,090 + GFF173 0.44 +0.1 164,158 - GFF173 0.48 +0.3 164,256 + GFF173 0.54 +0.1 164,257 - GFF173 0.55 -0.6
Or see this region's nucleotide sequence