Experiment: LB_plus_SM_buffer with Br60_phage 0.0000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1514 and GFF1515 overlap by 21 nucleotides GFF1515 and GFF1516 are separated by 130 nucleotides GFF1516 and GFF1517 are separated by 66 nucleotides GFF1517 and GFF1518 are separated by 9 nucleotides
GFF1514 - FIG01219785: hypothetical protein, at 19,865 to 20,308
GFF1514
GFF1515 - General stress protein 18, at 20,288 to 20,806
GFF1515
GFF1516 - Nucleoside-diphosphate-sugar epimerases, at 20,937 to 21,572
GFF1516
GFF1517 - 21 kDa hemolysin precursor, at 21,639 to 22,214
GFF1517
GFF1518 - Phosphoheptose isomerase (EC 5.3.1.-), at 22,224 to 22,814
GFF1518
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1 at 19.994 kb on + strand, within GFF1514 at 20.641 kb on + strand, within GFF1515 at 20.801 kb on - strand at 20.862 kb on - strand at 20.865 kb on + strand at 20.865 kb on + strand at 20.865 kb on + strand at 20.878 kb on - strand at 20.880 kb on + strand at 21.071 kb on + strand, within GFF1516 at 21.129 kb on - strand, within GFF1516 at 21.377 kb on + strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.570 kb on + strand at 21.609 kb on - strand at 21.640 kb on + strand at 21.644 kb on + strand at 21.645 kb on - strand at 21.645 kb on - strand at 21.645 kb on - strand at 21.647 kb on + strand at 21.648 kb on - strand at 21.653 kb on + strand at 21.653 kb on + strand at 21.891 kb on - strand, within GFF1517 at 21.892 kb on + strand, within GFF1517 at 21.925 kb on - strand, within GFF1517 at 21.972 kb on + strand, within GFF1517 at 22.170 kb on + strand at 22.170 kb on + strand at 22.226 kb on - strand at 22.226 kb on - strand at 22.290 kb on - strand, within GFF1518 at 22.356 kb on + strand, within GFF1518 at 22.357 kb on - strand, within GFF1518 at 22.558 kb on - strand, within GFF1518
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0000005 MOI remove 19,994 + GFF1514 0.29 +0.2 20,641 + GFF1515 0.68 +0.3 20,801 - -0.2 20,862 - +0.1 20,865 + -0.2 20,865 + +0.1 20,865 + -0.2 20,878 - +0.2 20,880 + -1.0 21,071 + GFF1516 0.21 +0.5 21,129 - GFF1516 0.30 +0.7 21,377 + GFF1516 0.69 -0.0 21,378 - GFF1516 0.69 +0.2 21,378 - GFF1516 0.69 +0.2 21,378 - GFF1516 0.69 +0.2 21,570 + -0.1 21,609 - -0.4 21,640 + +0.2 21,644 + -0.7 21,645 - -0.3 21,645 - +0.3 21,645 - +0.5 21,647 + -1.5 21,648 - +0.1 21,653 + +0.0 21,653 + +0.3 21,891 - GFF1517 0.44 +0.4 21,892 + GFF1517 0.44 +0.5 21,925 - GFF1517 0.50 +0.4 21,972 + GFF1517 0.58 +0.2 22,170 + -0.1 22,170 + -0.3 22,226 - +0.6 22,226 - +1.4 22,290 - GFF1518 0.11 -0.4 22,356 + GFF1518 0.22 -0.1 22,357 - GFF1518 0.23 +0.0 22,558 - GFF1518 0.57 -0.1
Or see this region's nucleotide sequence