Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu6064 and Atu6065 are separated by 68 nucleotides Atu6065 and Atu6066 are separated by 139 nucleotides
Atu6064: Atu6064 - haloalkane dehalogenase, at 76,616 to 77,458
Atu6064
Atu6065: Atu6065 - conserved hypothetical protein, at 77,527 to 78,150
Atu6065
Atu6066: Atu6066 - acyl-CoA dehydrogenase, at 78,290 to 79,495
Atu6066
Position (kb) 77
78
79 Strain fitness (log2 ratio) -2
-1
0
1
2 at 76.655 kb on + strand at 76.655 kb on - strand at 76.691 kb on + strand at 76.798 kb on + strand, within Atu6064 at 76.824 kb on - strand, within Atu6064 at 76.847 kb on + strand, within Atu6064 at 76.898 kb on - strand, within Atu6064 at 76.907 kb on - strand, within Atu6064 at 76.945 kb on + strand, within Atu6064 at 77.048 kb on + strand, within Atu6064 at 77.053 kb on + strand, within Atu6064 at 77.096 kb on + strand, within Atu6064 at 77.113 kb on - strand, within Atu6064 at 77.113 kb on - strand, within Atu6064 at 77.113 kb on - strand, within Atu6064 at 77.126 kb on + strand, within Atu6064 at 77.133 kb on + strand, within Atu6064 at 77.160 kb on + strand, within Atu6064 at 77.160 kb on + strand, within Atu6064 at 77.165 kb on + strand, within Atu6064 at 77.168 kb on - strand, within Atu6064 at 77.184 kb on + strand, within Atu6064 at 77.184 kb on + strand, within Atu6064 at 77.192 kb on - strand, within Atu6064 at 77.244 kb on + strand, within Atu6064 at 77.260 kb on + strand, within Atu6064 at 77.292 kb on + strand, within Atu6064 at 77.309 kb on - strand, within Atu6064 at 77.420 kb on + strand at 77.420 kb on + strand at 77.475 kb on - strand at 77.563 kb on + strand at 77.575 kb on + strand at 77.575 kb on + strand at 77.617 kb on - strand, within Atu6065 at 77.670 kb on + strand, within Atu6065 at 77.685 kb on - strand, within Atu6065 at 77.692 kb on - strand, within Atu6065 at 77.754 kb on - strand, within Atu6065 at 77.819 kb on - strand, within Atu6065 at 77.839 kb on + strand, within Atu6065 at 77.853 kb on + strand, within Atu6065 at 77.860 kb on + strand, within Atu6065 at 77.880 kb on - strand, within Atu6065 at 77.880 kb on - strand, within Atu6065 at 77.898 kb on + strand, within Atu6065 at 77.935 kb on + strand, within Atu6065 at 77.959 kb on - strand, within Atu6065 at 78.151 kb on + strand at 78.243 kb on + strand at 78.347 kb on + strand at 78.390 kb on - strand at 78.507 kb on - strand, within Atu6066 at 78.582 kb on + strand, within Atu6066 at 78.643 kb on + strand, within Atu6066 at 78.662 kb on - strand, within Atu6066 at 78.733 kb on + strand, within Atu6066 at 78.800 kb on + strand, within Atu6066 at 78.819 kb on + strand, within Atu6066 at 78.827 kb on - strand, within Atu6066 at 78.835 kb on + strand, within Atu6066 at 78.911 kb on + strand, within Atu6066 at 78.921 kb on - strand, within Atu6066 at 79.008 kb on + strand, within Atu6066 at 79.078 kb on + strand, within Atu6066 at 79.094 kb on + strand, within Atu6066
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 76,655 + +0.2 76,655 - +0.0 76,691 + -0.4 76,798 + Atu6064 0.22 -0.1 76,824 - Atu6064 0.25 +0.9 76,847 + Atu6064 0.27 +0.6 76,898 - Atu6064 0.33 +0.3 76,907 - Atu6064 0.35 +0.2 76,945 + Atu6064 0.39 -0.4 77,048 + Atu6064 0.51 +0.4 77,053 + Atu6064 0.52 -0.6 77,096 + Atu6064 0.57 +0.6 77,113 - Atu6064 0.59 +0.7 77,113 - Atu6064 0.59 +0.2 77,113 - Atu6064 0.59 -0.5 77,126 + Atu6064 0.60 -0.8 77,133 + Atu6064 0.61 -0.6 77,160 + Atu6064 0.65 +0.1 77,160 + Atu6064 0.65 -1.2 77,165 + Atu6064 0.65 +0.5 77,168 - Atu6064 0.65 -0.3 77,184 + Atu6064 0.67 +0.6 77,184 + Atu6064 0.67 -0.2 77,192 - Atu6064 0.68 -0.6 77,244 + Atu6064 0.74 +0.8 77,260 + Atu6064 0.76 +0.3 77,292 + Atu6064 0.80 -0.5 77,309 - Atu6064 0.82 +1.1 77,420 + +0.6 77,420 + -0.0 77,475 - -0.4 77,563 + +0.3 77,575 + -2.1 77,575 + -2.0 77,617 - Atu6065 0.14 +0.3 77,670 + Atu6065 0.23 -0.7 77,685 - Atu6065 0.25 -0.7 77,692 - Atu6065 0.26 +0.0 77,754 - Atu6065 0.36 +0.1 77,819 - Atu6065 0.47 -1.0 77,839 + Atu6065 0.50 -0.5 77,853 + Atu6065 0.52 -0.8 77,860 + Atu6065 0.53 -0.7 77,880 - Atu6065 0.57 -0.3 77,880 - Atu6065 0.57 -0.7 77,898 + Atu6065 0.59 +0.5 77,935 + Atu6065 0.65 -0.7 77,959 - Atu6065 0.69 -0.7 78,151 + +0.3 78,243 + +0.3 78,347 + +0.0 78,390 - -0.3 78,507 - Atu6066 0.18 -0.0 78,582 + Atu6066 0.24 -0.1 78,643 + Atu6066 0.29 +1.6 78,662 - Atu6066 0.31 +0.4 78,733 + Atu6066 0.37 +2.3 78,800 + Atu6066 0.42 -0.2 78,819 + Atu6066 0.44 +0.8 78,827 - Atu6066 0.45 -0.2 78,835 + Atu6066 0.45 +0.1 78,911 + Atu6066 0.51 -1.7 78,921 - Atu6066 0.52 +0.4 79,008 + Atu6066 0.60 +0.3 79,078 + Atu6066 0.65 +2.3 79,094 + Atu6066 0.67 +0.4
Or see this region's nucleotide sequence