Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5013 and Atu5014 overlap by 4 nucleotides Atu5014 and Atu5015 are separated by 14 nucleotides Atu5015 and Atu5016 are separated by 298 nucleotides
Atu5013: Atu5013 - hypothetical protein, at 14,416 to 14,664
Atu5013
Atu5014: Atu5014 - plasmid stabilization protein, at 14,661 to 15,074
Atu5014
Atu5015: Atu5015 - phage-related integrase, at 15,089 to 16,129
Atu5015
Atu5016: Atu5016 - hypothetical protein, at 16,428 to 17,576
Atu5016
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1
2 at 14.631 kb on + strand, within Atu5013 at 14.809 kb on + strand, within Atu5014 at 14.844 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.909 kb on - strand, within Atu5014 at 14.958 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.998 kb on - strand, within Atu5014 at 14.998 kb on - strand, within Atu5014 at 15.167 kb on - strand at 15.261 kb on + strand, within Atu5015 at 15.269 kb on - strand, within Atu5015 at 15.308 kb on - strand, within Atu5015 at 15.502 kb on + strand, within Atu5015 at 15.502 kb on + strand, within Atu5015 at 15.559 kb on + strand, within Atu5015 at 15.672 kb on - strand, within Atu5015 at 15.752 kb on + strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.770 kb on - strand, within Atu5015 at 15.802 kb on + strand, within Atu5015 at 15.993 kb on - strand, within Atu5015 at 15.995 kb on + strand, within Atu5015 at 15.998 kb on - strand, within Atu5015 at 15.998 kb on - strand, within Atu5015 at 16.035 kb on + strand at 16.035 kb on + strand at 16.045 kb on + strand at 16.066 kb on + strand at 16.066 kb on + strand at 16.107 kb on + strand at 16.211 kb on + strand at 16.219 kb on - strand at 16.267 kb on + strand at 16.513 kb on + strand at 16.550 kb on + strand, within Atu5016 at 16.558 kb on - strand, within Atu5016 at 16.560 kb on - strand, within Atu5016 at 16.580 kb on + strand, within Atu5016 at 16.580 kb on + strand, within Atu5016 at 16.583 kb on - strand, within Atu5016 at 16.588 kb on - strand, within Atu5016 at 16.595 kb on - strand, within Atu5016 at 16.627 kb on + strand, within Atu5016 at 16.712 kb on + strand, within Atu5016 at 16.737 kb on - strand, within Atu5016 at 16.808 kb on + strand, within Atu5016 at 16.824 kb on + strand, within Atu5016 at 16.923 kb on + strand, within Atu5016 at 16.942 kb on - strand, within Atu5016 at 17.000 kb on - strand, within Atu5016
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 14,631 + Atu5013 0.86 -0.2 14,809 + Atu5014 0.36 +0.1 14,844 + Atu5014 0.44 -0.0 14,901 + Atu5014 0.58 +1.2 14,901 + Atu5014 0.58 -0.3 14,901 + Atu5014 0.58 +1.0 14,901 + Atu5014 0.58 +0.3 14,909 - Atu5014 0.60 +0.5 14,958 + Atu5014 0.72 +0.3 14,990 + Atu5014 0.79 +0.4 14,990 + Atu5014 0.79 +0.8 14,990 + Atu5014 0.79 -0.0 14,990 + Atu5014 0.79 -0.6 14,998 - Atu5014 0.81 -1.8 14,998 - Atu5014 0.81 -1.0 15,167 - +0.1 15,261 + Atu5015 0.17 -1.2 15,269 - Atu5015 0.17 -0.4 15,308 - Atu5015 0.21 +2.0 15,502 + Atu5015 0.40 -0.2 15,502 + Atu5015 0.40 -0.2 15,559 + Atu5015 0.45 -0.4 15,672 - Atu5015 0.56 +0.5 15,752 + Atu5015 0.64 -0.7 15,760 - Atu5015 0.64 +1.0 15,760 - Atu5015 0.64 -0.2 15,760 - Atu5015 0.64 +0.4 15,770 - Atu5015 0.65 +1.3 15,802 + Atu5015 0.68 +1.5 15,993 - Atu5015 0.87 +0.2 15,995 + Atu5015 0.87 +0.3 15,998 - Atu5015 0.87 +0.5 15,998 - Atu5015 0.87 -0.0 16,035 + -0.1 16,035 + +1.0 16,045 + -1.0 16,066 + +0.1 16,066 + -0.4 16,107 + -0.7 16,211 + +0.4 16,219 - +0.3 16,267 + +0.3 16,513 + +0.1 16,550 + Atu5016 0.11 -0.5 16,558 - Atu5016 0.11 -0.9 16,560 - Atu5016 0.11 +0.2 16,580 + Atu5016 0.13 +0.1 16,580 + Atu5016 0.13 -1.1 16,583 - Atu5016 0.13 -1.0 16,588 - Atu5016 0.14 +0.6 16,595 - Atu5016 0.15 +0.2 16,627 + Atu5016 0.17 +0.1 16,712 + Atu5016 0.25 +0.6 16,737 - Atu5016 0.27 -0.3 16,808 + Atu5016 0.33 +0.2 16,824 + Atu5016 0.34 +0.8 16,923 + Atu5016 0.43 -1.3 16,942 - Atu5016 0.45 -0.5 17,000 - Atu5016 0.50 +0.9
Or see this region's nucleotide sequence