Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4044 and Atu4045 overlap by 8 nucleotides Atu4045 and Atu4046 are separated by 218 nucleotides
Atu4044: Atu4044 - glycine betaine ABC transporter ATPase, at 1,157,179 to 1,158,240
Atu4044
Atu4045: Atu4045 - glycine betaine ABC transporter permease, at 1,158,233 to 1,159,120
Atu4045
Atu4046: Atu4046 - glycine betaine ABC transporter substrate-binding protein, at 1,159,339 to 1,160,337
Atu4046
Position (kb)
1158
1159
1160 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1157.233 kb on - strand at 1157.249 kb on - strand at 1157.303 kb on - strand, within Atu4044 at 1157.457 kb on - strand, within Atu4044 at 1157.469 kb on - strand, within Atu4044 at 1157.660 kb on + strand, within Atu4044 at 1157.668 kb on - strand, within Atu4044 at 1157.668 kb on - strand, within Atu4044 at 1157.668 kb on - strand, within Atu4044 at 1157.718 kb on - strand, within Atu4044 at 1157.753 kb on + strand, within Atu4044 at 1157.761 kb on - strand, within Atu4044 at 1157.761 kb on - strand, within Atu4044 at 1157.786 kb on + strand, within Atu4044 at 1157.799 kb on - strand, within Atu4044 at 1157.833 kb on + strand, within Atu4044 at 1157.841 kb on - strand, within Atu4044 at 1158.025 kb on + strand, within Atu4044 at 1158.056 kb on + strand, within Atu4044 at 1158.062 kb on - strand, within Atu4044 at 1158.108 kb on + strand, within Atu4044 at 1158.114 kb on + strand, within Atu4044 at 1158.122 kb on - strand, within Atu4044 at 1158.122 kb on - strand, within Atu4044 at 1158.122 kb on - strand, within Atu4044 at 1158.131 kb on + strand, within Atu4044 at 1158.139 kb on - strand at 1158.139 kb on - strand at 1158.143 kb on + strand at 1158.151 kb on - strand at 1158.151 kb on - strand at 1158.151 kb on - strand at 1158.271 kb on + strand at 1158.281 kb on - strand at 1158.286 kb on - strand at 1158.382 kb on + strand, within Atu4045 at 1158.382 kb on + strand, within Atu4045 at 1158.382 kb on + strand, within Atu4045 at 1158.382 kb on + strand, within Atu4045 at 1158.390 kb on - strand, within Atu4045 at 1158.390 kb on - strand, within Atu4045 at 1158.390 kb on - strand, within Atu4045 at 1158.492 kb on + strand, within Atu4045 at 1158.500 kb on - strand, within Atu4045 at 1158.500 kb on - strand, within Atu4045 at 1158.514 kb on + strand, within Atu4045 at 1158.546 kb on - strand, within Atu4045 at 1158.634 kb on + strand, within Atu4045 at 1158.743 kb on + strand, within Atu4045 at 1158.882 kb on - strand, within Atu4045 at 1158.983 kb on + strand, within Atu4045 at 1158.983 kb on + strand, within Atu4045 at 1158.988 kb on + strand, within Atu4045 at 1158.993 kb on + strand, within Atu4045 at 1158.996 kb on + strand, within Atu4045 at 1159.030 kb on + strand, within Atu4045 at 1159.116 kb on + strand at 1159.131 kb on + strand at 1159.152 kb on + strand at 1159.154 kb on + strand at 1159.154 kb on + strand at 1159.162 kb on - strand at 1159.162 kb on - strand at 1159.162 kb on - strand at 1159.162 kb on - strand at 1159.326 kb on - strand at 1159.329 kb on - strand at 1159.357 kb on - strand at 1159.379 kb on + strand at 1159.387 kb on - strand at 1159.513 kb on + strand, within Atu4046 at 1159.513 kb on + strand, within Atu4046 at 1159.513 kb on + strand, within Atu4046 at 1159.521 kb on - strand, within Atu4046 at 1159.573 kb on + strand, within Atu4046 at 1159.736 kb on + strand, within Atu4046 at 1159.781 kb on + strand, within Atu4046 at 1159.850 kb on - strand, within Atu4046 at 1159.853 kb on + strand, within Atu4046 at 1159.921 kb on - strand, within Atu4046 at 1159.932 kb on + strand, within Atu4046 at 1159.932 kb on + strand, within Atu4046 at 1159.990 kb on + strand, within Atu4046 at 1159.995 kb on + strand, within Atu4046 at 1159.998 kb on - strand, within Atu4046 at 1159.998 kb on - strand, within Atu4046 at 1160.051 kb on - strand, within Atu4046
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,157,233 - -0.0 1,157,249 - +0.4 1,157,303 - Atu4044 0.12 -0.2 1,157,457 - Atu4044 0.26 +0.4 1,157,469 - Atu4044 0.27 -2.3 1,157,660 + Atu4044 0.45 -0.1 1,157,668 - Atu4044 0.46 +1.1 1,157,668 - Atu4044 0.46 +0.9 1,157,668 - Atu4044 0.46 -0.2 1,157,718 - Atu4044 0.51 +0.5 1,157,753 + Atu4044 0.54 -1.0 1,157,761 - Atu4044 0.55 +0.1 1,157,761 - Atu4044 0.55 -0.0 1,157,786 + Atu4044 0.57 -0.1 1,157,799 - Atu4044 0.58 -0.5 1,157,833 + Atu4044 0.62 +0.1 1,157,841 - Atu4044 0.62 +0.2 1,158,025 + Atu4044 0.80 -0.6 1,158,056 + Atu4044 0.83 +0.5 1,158,062 - Atu4044 0.83 -0.5 1,158,108 + Atu4044 0.87 +1.6 1,158,114 + Atu4044 0.88 +0.7 1,158,122 - Atu4044 0.89 +0.8 1,158,122 - Atu4044 0.89 -0.3 1,158,122 - Atu4044 0.89 -0.1 1,158,131 + Atu4044 0.90 +1.5 1,158,139 - +2.1 1,158,139 - +0.6 1,158,143 + +0.3 1,158,151 - +1.5 1,158,151 - -0.3 1,158,151 - +0.1 1,158,271 + +0.3 1,158,281 - -0.0 1,158,286 - -1.9 1,158,382 + Atu4045 0.17 -0.0 1,158,382 + Atu4045 0.17 +0.1 1,158,382 + Atu4045 0.17 +0.5 1,158,382 + Atu4045 0.17 -0.5 1,158,390 - Atu4045 0.18 +0.5 1,158,390 - Atu4045 0.18 +0.4 1,158,390 - Atu4045 0.18 +0.5 1,158,492 + Atu4045 0.29 +0.5 1,158,500 - Atu4045 0.30 +0.1 1,158,500 - Atu4045 0.30 -0.0 1,158,514 + Atu4045 0.32 +0.7 1,158,546 - Atu4045 0.35 +1.0 1,158,634 + Atu4045 0.45 +1.0 1,158,743 + Atu4045 0.57 -0.4 1,158,882 - Atu4045 0.73 +0.2 1,158,983 + Atu4045 0.84 -0.2 1,158,983 + Atu4045 0.84 +1.3 1,158,988 + Atu4045 0.85 +0.4 1,158,993 + Atu4045 0.86 +0.5 1,158,996 + Atu4045 0.86 +0.8 1,159,030 + Atu4045 0.90 +0.1 1,159,116 + +0.2 1,159,131 + -1.0 1,159,152 + +0.8 1,159,154 + -0.1 1,159,154 + -0.2 1,159,162 - +1.1 1,159,162 - -0.2 1,159,162 - +0.1 1,159,162 - +0.5 1,159,326 - +0.6 1,159,329 - -0.3 1,159,357 - +1.1 1,159,379 + +0.4 1,159,387 - -0.3 1,159,513 + Atu4046 0.17 -0.1 1,159,513 + Atu4046 0.17 -0.1 1,159,513 + Atu4046 0.17 +0.4 1,159,521 - Atu4046 0.18 +0.1 1,159,573 + Atu4046 0.23 +0.4 1,159,736 + Atu4046 0.40 +0.1 1,159,781 + Atu4046 0.44 -0.3 1,159,850 - Atu4046 0.51 +0.1 1,159,853 + Atu4046 0.51 -0.2 1,159,921 - Atu4046 0.58 -0.2 1,159,932 + Atu4046 0.59 -0.4 1,159,932 + Atu4046 0.59 +0.6 1,159,990 + Atu4046 0.65 +0.4 1,159,995 + Atu4046 0.66 -0.1 1,159,998 - Atu4046 0.66 +0.3 1,159,998 - Atu4046 0.66 -0.4 1,160,051 - Atu4046 0.71 -0.1
Or see this region's nucleotide sequence