Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3971 and Atu3972 are separated by 9 nucleotides Atu3972 and Atu3973 are separated by 33 nucleotides Atu3973 and Atu3974 are separated by 108 nucleotides
Atu3971: Atu3971 - dihydroxy-acid dehydratase, at 1,075,979 to 1,077,703
Atu3971
Atu3972: Atu3972 - hypothetical protein, at 1,077,713 to 1,078,510
Atu3972
Atu3973: Atu3973 - hypothetical protein, at 1,078,544 to 1,079,254
Atu3973
Atu3974: Atu3974 - ice nucleation-like protein, at 1,079,363 to 1,084,558
Atu3974
Position (kb)
1078
1079
1080 Strain fitness (log2 ratio)
-1
0
1 at 1077.597 kb on + strand at 1077.640 kb on - strand at 1077.781 kb on - strand at 1077.796 kb on - strand, within Atu3972 at 1077.944 kb on + strand, within Atu3972 at 1077.952 kb on - strand, within Atu3972 at 1077.962 kb on - strand, within Atu3972 at 1077.990 kb on - strand, within Atu3972 at 1078.056 kb on + strand, within Atu3972 at 1078.079 kb on - strand, within Atu3972 at 1078.104 kb on + strand, within Atu3972 at 1078.263 kb on - strand, within Atu3972 at 1078.284 kb on + strand, within Atu3972 at 1078.315 kb on + strand, within Atu3972 at 1078.438 kb on - strand at 1078.541 kb on + strand at 1078.549 kb on - strand at 1078.639 kb on - strand, within Atu3973 at 1078.664 kb on + strand, within Atu3973 at 1078.694 kb on - strand, within Atu3973 at 1078.696 kb on + strand, within Atu3973 at 1078.799 kb on - strand, within Atu3973 at 1079.158 kb on + strand, within Atu3973 at 1079.161 kb on - strand, within Atu3973 at 1079.166 kb on - strand, within Atu3973 at 1079.166 kb on - strand, within Atu3973 at 1079.184 kb on + strand at 1079.184 kb on + strand at 1079.191 kb on + strand at 1079.373 kb on + strand at 1079.462 kb on - strand at 1079.462 kb on - strand at 1079.497 kb on - strand at 1079.563 kb on - strand at 1079.589 kb on + strand at 1079.728 kb on + strand at 1079.747 kb on + strand at 1079.848 kb on + strand at 1079.848 kb on + strand at 1079.896 kb on + strand, within Atu3974 at 1079.973 kb on - strand, within Atu3974 at 1079.983 kb on - strand, within Atu3974 at 1079.987 kb on + strand, within Atu3974 at 1080.012 kb on + strand, within Atu3974 at 1080.017 kb on + strand, within Atu3974 at 1080.025 kb on - strand, within Atu3974 at 1080.098 kb on - strand, within Atu3974 at 1080.105 kb on + strand, within Atu3974 at 1080.182 kb on - strand, within Atu3974 at 1080.194 kb on - strand, within Atu3974 at 1080.205 kb on - strand, within Atu3974 at 1080.253 kb on - strand, within Atu3974 at 1080.254 kb on - strand, within Atu3974
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,077,597 + -0.1 1,077,640 - +0.2 1,077,781 - +0.2 1,077,796 - Atu3972 0.10 +0.0 1,077,944 + Atu3972 0.29 -0.5 1,077,952 - Atu3972 0.30 +0.6 1,077,962 - Atu3972 0.31 +1.5 1,077,990 - Atu3972 0.35 -0.4 1,078,056 + Atu3972 0.43 +0.1 1,078,079 - Atu3972 0.46 +0.5 1,078,104 + Atu3972 0.49 +0.0 1,078,263 - Atu3972 0.69 +0.7 1,078,284 + Atu3972 0.72 -0.3 1,078,315 + Atu3972 0.75 -0.4 1,078,438 - -0.2 1,078,541 + -0.0 1,078,549 - +0.8 1,078,639 - Atu3973 0.13 -0.2 1,078,664 + Atu3973 0.17 +1.3 1,078,694 - Atu3973 0.21 -0.7 1,078,696 + Atu3973 0.21 -0.1 1,078,799 - Atu3973 0.36 +0.1 1,079,158 + Atu3973 0.86 +0.4 1,079,161 - Atu3973 0.87 +0.7 1,079,166 - Atu3973 0.87 +1.6 1,079,166 - Atu3973 0.87 +1.7 1,079,184 + +0.0 1,079,184 + +0.1 1,079,191 + +0.9 1,079,373 + +0.4 1,079,462 - -0.2 1,079,462 - +0.6 1,079,497 - -0.5 1,079,563 - +0.0 1,079,589 + -0.3 1,079,728 + +0.5 1,079,747 + +0.1 1,079,848 + -0.7 1,079,848 + +0.6 1,079,896 + Atu3974 0.10 +0.3 1,079,973 - Atu3974 0.12 -0.2 1,079,983 - Atu3974 0.12 +0.2 1,079,987 + Atu3974 0.12 +1.2 1,080,012 + Atu3974 0.12 -1.0 1,080,017 + Atu3974 0.13 +0.0 1,080,025 - Atu3974 0.13 -0.4 1,080,098 - Atu3974 0.14 +0.2 1,080,105 + Atu3974 0.14 +0.3 1,080,182 - Atu3974 0.16 +0.0 1,080,194 - Atu3974 0.16 -0.5 1,080,205 - Atu3974 0.16 +0.6 1,080,253 - Atu3974 0.17 +0.1 1,080,254 - Atu3974 0.17 -0.0
Or see this region's nucleotide sequence