Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3490 and Atu3491 are separated by 3 nucleotides Atu3491 and Atu3492 overlap by 4 nucleotides
Atu3490: Atu3490 - ABC transporter permease, at 541,189 to 542,199
Atu3490
Atu3491: Atu3491 - hypothetical protein, at 542,203 to 542,526
Atu3491
Atu3492: Atu3492 - sugar kinase, at 542,523 to 543,905
Atu3492
Position (kb) 542
543 Strain fitness (log2 ratio) -2
-1
0
1
2 at 541.264 kb on - strand at 541.377 kb on - strand, within Atu3490 at 541.377 kb on - strand, within Atu3490 at 541.377 kb on - strand, within Atu3490 at 541.422 kb on - strand, within Atu3490 at 541.422 kb on - strand, within Atu3490 at 541.462 kb on + strand, within Atu3490 at 541.492 kb on + strand, within Atu3490 at 541.500 kb on - strand, within Atu3490 at 541.500 kb on - strand, within Atu3490 at 541.505 kb on - strand, within Atu3490 at 541.511 kb on + strand, within Atu3490 at 541.516 kb on + strand, within Atu3490 at 541.519 kb on - strand, within Atu3490 at 541.519 kb on - strand, within Atu3490 at 541.603 kb on + strand, within Atu3490 at 541.619 kb on + strand, within Atu3490 at 541.647 kb on - strand, within Atu3490 at 541.715 kb on - strand, within Atu3490 at 541.715 kb on - strand, within Atu3490 at 541.806 kb on + strand, within Atu3490 at 541.813 kb on - strand, within Atu3490 at 541.814 kb on - strand, within Atu3490 at 541.814 kb on - strand, within Atu3490 at 541.902 kb on - strand, within Atu3490 at 542.150 kb on - strand at 542.184 kb on + strand at 542.193 kb on + strand at 542.193 kb on + strand at 542.201 kb on - strand at 542.201 kb on - strand at 542.278 kb on + strand, within Atu3491 at 542.296 kb on - strand, within Atu3491 at 542.341 kb on + strand, within Atu3491 at 542.384 kb on + strand, within Atu3491 at 542.502 kb on + strand at 542.533 kb on + strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.587 kb on - strand at 542.660 kb on + strand at 542.926 kb on - strand, within Atu3492 at 542.985 kb on + strand, within Atu3492 at 543.061 kb on + strand, within Atu3492 at 543.071 kb on + strand, within Atu3492 at 543.221 kb on + strand, within Atu3492
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 541,264 - -0.3 541,377 - Atu3490 0.19 -0.2 541,377 - Atu3490 0.19 +0.6 541,377 - Atu3490 0.19 +0.1 541,422 - Atu3490 0.23 +0.6 541,422 - Atu3490 0.23 -1.1 541,462 + Atu3490 0.27 -1.1 541,492 + Atu3490 0.30 -0.5 541,500 - Atu3490 0.31 -0.5 541,500 - Atu3490 0.31 +0.6 541,505 - Atu3490 0.31 -0.3 541,511 + Atu3490 0.32 +1.1 541,516 + Atu3490 0.32 -1.8 541,519 - Atu3490 0.33 -0.2 541,519 - Atu3490 0.33 -0.1 541,603 + Atu3490 0.41 -0.0 541,619 + Atu3490 0.43 -0.1 541,647 - Atu3490 0.45 -0.9 541,715 - Atu3490 0.52 +0.0 541,715 - Atu3490 0.52 -0.7 541,806 + Atu3490 0.61 +1.4 541,813 - Atu3490 0.62 -0.2 541,814 - Atu3490 0.62 +0.7 541,814 - Atu3490 0.62 +0.1 541,902 - Atu3490 0.71 +0.4 542,150 - -0.1 542,184 + +0.8 542,193 + +0.6 542,193 + +0.3 542,201 - -0.5 542,201 - -0.5 542,278 + Atu3491 0.23 -1.6 542,296 - Atu3491 0.29 -0.9 542,341 + Atu3491 0.43 +0.9 542,384 + Atu3491 0.56 +0.0 542,502 + -0.3 542,533 + +0.2 542,541 - +1.8 542,541 - +0.5 542,541 - -0.2 542,541 - -0.2 542,541 - +0.7 542,541 - -0.2 542,587 - +0.1 542,660 + +1.0 542,926 - Atu3492 0.29 +0.1 542,985 + Atu3492 0.33 -0.4 543,061 + Atu3492 0.39 -0.6 543,071 + Atu3492 0.40 -0.5 543,221 + Atu3492 0.50 +0.2
Or see this region's nucleotide sequence