Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2798 and Atu2799 overlap by 2 nucleotides Atu2799 and Atu2800 overlap by 34 nucleotides Atu2800 and Atu2801 overlap by 4 nucleotides
Atu2798: Atu2798 - precorrin-6y methyltransferase, at 2,799,463 to 2,800,722
Atu2798
Atu2799: Atu2799 - precorrin-6x reductase, at 2,800,721 to 2,801,503
Atu2799
Atu2800: Atu2800 - precorrin-3B C17-methyltransferase, at 2,801,470 to 2,802,231
Atu2800
Atu2801: Atu2801 - precorrin-2 C20 methyltransferase, at 2,802,228 to 2,802,995
Atu2801
Position (kb)
2800
2801
2802 Strain fitness (log2 ratio)
-1
0
1
2 at 2799.810 kb on + strand, within Atu2798 at 2800.048 kb on - strand, within Atu2798 at 2800.224 kb on + strand, within Atu2798 at 2800.286 kb on - strand, within Atu2798 at 2800.424 kb on + strand, within Atu2798 at 2800.514 kb on + strand, within Atu2798 at 2800.527 kb on - strand, within Atu2798 at 2800.643 kb on + strand at 2800.693 kb on - strand at 2800.729 kb on + strand at 2800.736 kb on - strand at 2800.821 kb on - strand, within Atu2799 at 2800.847 kb on - strand, within Atu2799 at 2800.892 kb on - strand, within Atu2799 at 2800.948 kb on - strand, within Atu2799 at 2801.057 kb on - strand, within Atu2799 at 2801.078 kb on - strand, within Atu2799 at 2801.103 kb on - strand, within Atu2799 at 2801.230 kb on + strand, within Atu2799 at 2801.367 kb on + strand, within Atu2799 at 2801.445 kb on + strand at 2801.735 kb on + strand, within Atu2800 at 2801.931 kb on - strand, within Atu2800 at 2802.095 kb on + strand, within Atu2800 at 2802.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,799,810 + Atu2798 0.28 -0.9 2,800,048 - Atu2798 0.46 -1.2 2,800,224 + Atu2798 0.60 -1.5 2,800,286 - Atu2798 0.65 -1.1 2,800,424 + Atu2798 0.76 -0.0 2,800,514 + Atu2798 0.83 -0.8 2,800,527 - Atu2798 0.84 +0.9 2,800,643 + -1.3 2,800,693 - -0.3 2,800,729 + +0.2 2,800,736 - -1.1 2,800,821 - Atu2799 0.13 -0.1 2,800,847 - Atu2799 0.16 -1.5 2,800,892 - Atu2799 0.22 -0.5 2,800,948 - Atu2799 0.29 +2.0 2,801,057 - Atu2799 0.43 -1.0 2,801,078 - Atu2799 0.46 -1.5 2,801,103 - Atu2799 0.49 -0.3 2,801,230 + Atu2799 0.65 +0.1 2,801,367 + Atu2799 0.83 +0.7 2,801,445 + +0.0 2,801,735 + Atu2800 0.35 -0.8 2,801,931 - Atu2800 0.60 -1.6 2,802,095 + Atu2800 0.82 +0.1 2,802,235 - +0.3
Or see this region's nucleotide sequence