Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2582 and Atu2583 are separated by 193 nucleotides Atu2583 and Atu2584 overlap by 4 nucleotides Atu2584 and Atu2585 overlap by 9 nucleotides
Atu2582: Atu2582 - UDP-glucose/GDP-mannose dehydrogenase, at 2,556,795 to 2,558,069
Atu2582
Atu2583: Atu2583 - glycosyltransferase, at 2,558,263 to 2,559,360
Atu2583
Atu2584: Atu2584 - hypothetical protein, at 2,559,357 to 2,560,100
Atu2584
Atu2585: Atu2585 - hypothetical protein, at 2,560,092 to 2,560,931
Atu2585
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2557.319 kb on + strand, within Atu2582 at 2557.363 kb on - strand, within Atu2582 at 2557.422 kb on - strand, within Atu2582 at 2557.573 kb on + strand, within Atu2582 at 2557.678 kb on - strand, within Atu2582 at 2557.680 kb on + strand, within Atu2582 at 2557.682 kb on + strand, within Atu2582 at 2557.698 kb on - strand, within Atu2582 at 2557.725 kb on - strand, within Atu2582 at 2557.758 kb on - strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582 at 2557.933 kb on - strand, within Atu2582 at 2558.058 kb on - strand at 2558.128 kb on - strand at 2558.138 kb on - strand at 2558.203 kb on + strand at 2558.247 kb on + strand at 2558.306 kb on - strand at 2558.373 kb on + strand, within Atu2583 at 2558.384 kb on + strand, within Atu2583 at 2558.389 kb on + strand, within Atu2583 at 2558.392 kb on - strand, within Atu2583 at 2558.392 kb on - strand, within Atu2583 at 2558.432 kb on - strand, within Atu2583 at 2558.437 kb on - strand, within Atu2583 at 2558.698 kb on + strand, within Atu2583 at 2558.705 kb on + strand, within Atu2583 at 2558.707 kb on + strand, within Atu2583 at 2558.786 kb on + strand, within Atu2583 at 2558.814 kb on + strand, within Atu2583 at 2558.822 kb on - strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.879 kb on - strand, within Atu2583 at 2558.886 kb on - strand, within Atu2583 at 2558.900 kb on - strand, within Atu2583 at 2559.072 kb on + strand, within Atu2583 at 2559.073 kb on + strand, within Atu2583 at 2559.112 kb on - strand, within Atu2583 at 2559.118 kb on + strand, within Atu2583 at 2559.118 kb on + strand, within Atu2583 at 2559.172 kb on + strand, within Atu2583 at 2559.229 kb on - strand, within Atu2583 at 2559.236 kb on - strand, within Atu2583 at 2559.244 kb on - strand, within Atu2583 at 2559.246 kb on + strand, within Atu2583 at 2559.361 kb on - strand at 2559.700 kb on - strand, within Atu2584 at 2559.710 kb on + strand, within Atu2584 at 2559.734 kb on - strand, within Atu2584 at 2559.848 kb on + strand, within Atu2584 at 2559.928 kb on + strand, within Atu2584 at 2559.943 kb on + strand, within Atu2584 at 2560.012 kb on + strand, within Atu2584 at 2560.022 kb on + strand, within Atu2584 at 2560.036 kb on + strand at 2560.074 kb on + strand at 2560.115 kb on + strand at 2560.128 kb on - strand at 2560.219 kb on - strand, within Atu2585 at 2560.225 kb on - strand, within Atu2585 at 2560.246 kb on + strand, within Atu2585 at 2560.275 kb on - strand, within Atu2585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,557,319 + Atu2582 0.41 -2.3 2,557,363 - Atu2582 0.45 -0.9 2,557,422 - Atu2582 0.49 -1.8 2,557,573 + Atu2582 0.61 -2.5 2,557,678 - Atu2582 0.69 -1.5 2,557,680 + Atu2582 0.69 -1.1 2,557,682 + Atu2582 0.70 -1.6 2,557,698 - Atu2582 0.71 -2.9 2,557,725 - Atu2582 0.73 -1.7 2,557,758 - Atu2582 0.76 -1.5 2,557,760 + Atu2582 0.76 -1.1 2,557,760 + Atu2582 0.76 -0.3 2,557,933 - Atu2582 0.89 +1.2 2,558,058 - +0.7 2,558,128 - -0.9 2,558,138 - -1.1 2,558,203 + +0.7 2,558,247 + -0.1 2,558,306 - -3.1 2,558,373 + Atu2583 0.10 -2.3 2,558,384 + Atu2583 0.11 -3.7 2,558,389 + Atu2583 0.11 -2.9 2,558,392 - Atu2583 0.12 +2.3 2,558,392 - Atu2583 0.12 +1.3 2,558,432 - Atu2583 0.15 -3.6 2,558,437 - Atu2583 0.16 -0.3 2,558,698 + Atu2583 0.40 +0.7 2,558,705 + Atu2583 0.40 -0.7 2,558,707 + Atu2583 0.40 -3.2 2,558,786 + Atu2583 0.48 -2.2 2,558,814 + Atu2583 0.50 -0.1 2,558,822 - Atu2583 0.51 -2.4 2,558,861 + Atu2583 0.54 -1.6 2,558,861 + Atu2583 0.54 -3.2 2,558,861 + Atu2583 0.54 +0.3 2,558,861 + Atu2583 0.54 -4.0 2,558,861 + Atu2583 0.54 +0.7 2,558,861 + Atu2583 0.54 -4.0 2,558,861 + Atu2583 0.54 +1.3 2,558,861 + Atu2583 0.54 -2.1 2,558,869 - Atu2583 0.55 -2.8 2,558,869 - Atu2583 0.55 -1.4 2,558,869 - Atu2583 0.55 -2.1 2,558,869 - Atu2583 0.55 -0.6 2,558,869 - Atu2583 0.55 -1.4 2,558,869 - Atu2583 0.55 +0.3 2,558,869 - Atu2583 0.55 -1.8 2,558,869 - Atu2583 0.55 -0.1 2,558,879 - Atu2583 0.56 -1.1 2,558,886 - Atu2583 0.57 -4.3 2,558,900 - Atu2583 0.58 -0.7 2,559,072 + Atu2583 0.74 -4.2 2,559,073 + Atu2583 0.74 -0.1 2,559,112 - Atu2583 0.77 -1.1 2,559,118 + Atu2583 0.78 +0.7 2,559,118 + Atu2583 0.78 +0.7 2,559,172 + Atu2583 0.83 -2.7 2,559,229 - Atu2583 0.88 -0.9 2,559,236 - Atu2583 0.89 -3.3 2,559,244 - Atu2583 0.89 -2.1 2,559,246 + Atu2583 0.90 +0.7 2,559,361 - +0.5 2,559,700 - Atu2584 0.46 -1.9 2,559,710 + Atu2584 0.47 -4.3 2,559,734 - Atu2584 0.51 +1.3 2,559,848 + Atu2584 0.66 -2.9 2,559,928 + Atu2584 0.77 -2.3 2,559,943 + Atu2584 0.79 -0.1 2,560,012 + Atu2584 0.88 +0.7 2,560,022 + Atu2584 0.89 -2.7 2,560,036 + -0.7 2,560,074 + -4.3 2,560,115 + -2.8 2,560,128 - -4.0 2,560,219 - Atu2585 0.15 -0.9 2,560,225 - Atu2585 0.16 -1.4 2,560,246 + Atu2585 0.18 +1.3 2,560,275 - Atu2585 0.22 +2.3
Or see this region's nucleotide sequence