Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2573 and Atu2574 are separated by 342 nucleotides Atu2574 and Atu2575 overlap by 4 nucleotides Atu2575 and Atu2576 overlap by 33 nucleotides
Atu2573: Atu2573 - hypothetical protein, at 2,543,770 to 2,544,465
Atu2573
Atu2574: Atu2574 - hypothetical protein, at 2,544,808 to 2,545,455
Atu2574
Atu2575: Atu2575 - mannosidase, at 2,545,452 to 2,547,908
Atu2575
Atu2576: Atu2576 - hypothetical protein, at 2,547,876 to 2,549,735
Atu2576
Position (kb)
2545
2546
2547
2548 Strain fitness (log2 ratio)
-1
0
1
2 at 2544.618 kb on + strand at 2544.800 kb on + strand at 2544.811 kb on + strand at 2544.826 kb on - strand at 2544.833 kb on + strand at 2544.854 kb on - strand at 2545.023 kb on + strand, within Atu2574 at 2545.247 kb on - strand, within Atu2574 at 2545.259 kb on - strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.305 kb on + strand, within Atu2574 at 2545.374 kb on + strand, within Atu2574 at 2545.411 kb on - strand at 2545.432 kb on + strand at 2545.499 kb on - strand at 2545.589 kb on - strand at 2545.693 kb on + strand at 2545.710 kb on - strand, within Atu2575 at 2545.781 kb on - strand, within Atu2575 at 2545.819 kb on + strand, within Atu2575 at 2545.928 kb on + strand, within Atu2575 at 2545.933 kb on + strand, within Atu2575 at 2545.933 kb on + strand, within Atu2575 at 2545.939 kb on - strand, within Atu2575 at 2545.941 kb on + strand, within Atu2575 at 2545.986 kb on + strand, within Atu2575 at 2546.027 kb on + strand, within Atu2575 at 2546.035 kb on + strand, within Atu2575 at 2546.035 kb on - strand, within Atu2575 at 2546.042 kb on + strand, within Atu2575 at 2546.072 kb on + strand, within Atu2575 at 2546.106 kb on - strand, within Atu2575 at 2546.237 kb on + strand, within Atu2575 at 2546.245 kb on - strand, within Atu2575 at 2546.250 kb on - strand, within Atu2575 at 2546.300 kb on + strand, within Atu2575 at 2546.308 kb on - strand, within Atu2575 at 2546.308 kb on - strand, within Atu2575 at 2546.353 kb on - strand, within Atu2575 at 2546.353 kb on - strand, within Atu2575 at 2546.386 kb on - strand, within Atu2575 at 2546.386 kb on - strand, within Atu2575 at 2546.726 kb on + strand, within Atu2575 at 2546.836 kb on + strand, within Atu2575 at 2546.969 kb on + strand, within Atu2575 at 2546.977 kb on - strand, within Atu2575 at 2547.083 kb on + strand, within Atu2575 at 2547.143 kb on + strand, within Atu2575 at 2547.295 kb on - strand, within Atu2575 at 2547.330 kb on + strand, within Atu2575 at 2547.343 kb on - strand, within Atu2575 at 2547.387 kb on - strand, within Atu2575 at 2547.446 kb on - strand, within Atu2575 at 2547.477 kb on - strand, within Atu2575 at 2547.500 kb on - strand, within Atu2575 at 2547.539 kb on + strand, within Atu2575 at 2547.547 kb on - strand, within Atu2575 at 2547.547 kb on - strand, within Atu2575 at 2547.736 kb on - strand at 2547.747 kb on + strand at 2547.755 kb on - strand at 2547.772 kb on - strand at 2547.772 kb on - strand at 2547.807 kb on - strand at 2547.968 kb on + strand at 2548.083 kb on - strand, within Atu2576 at 2548.140 kb on + strand, within Atu2576 at 2548.188 kb on - strand, within Atu2576 at 2548.312 kb on + strand, within Atu2576 at 2548.504 kb on + strand, within Atu2576 at 2548.596 kb on + strand, within Atu2576 at 2548.662 kb on + strand, within Atu2576 at 2548.717 kb on + strand, within Atu2576 at 2548.752 kb on + strand, within Atu2576 at 2548.775 kb on + strand, within Atu2576 at 2548.843 kb on + strand, within Atu2576 at 2548.843 kb on + strand, within Atu2576 at 2548.851 kb on - strand, within Atu2576 at 2548.861 kb on - strand, within Atu2576
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,544,618 + -0.1 2,544,800 + +1.4 2,544,811 + -0.1 2,544,826 - +0.6 2,544,833 + +0.0 2,544,854 - -0.6 2,545,023 + Atu2574 0.33 -0.1 2,545,247 - Atu2574 0.68 -0.1 2,545,259 - Atu2574 0.70 -1.2 2,545,305 + Atu2574 0.77 +0.2 2,545,305 + Atu2574 0.77 +0.3 2,545,305 + Atu2574 0.77 +0.0 2,545,374 + Atu2574 0.87 +0.3 2,545,411 - -0.3 2,545,432 + +0.4 2,545,499 - -0.2 2,545,589 - +0.2 2,545,693 + -0.3 2,545,710 - Atu2575 0.11 -0.8 2,545,781 - Atu2575 0.13 +0.6 2,545,819 + Atu2575 0.15 -1.2 2,545,928 + Atu2575 0.19 +0.1 2,545,933 + Atu2575 0.20 +0.8 2,545,933 + Atu2575 0.20 +1.2 2,545,939 - Atu2575 0.20 -0.2 2,545,941 + Atu2575 0.20 +0.6 2,545,986 + Atu2575 0.22 -0.3 2,546,027 + Atu2575 0.23 -0.1 2,546,035 + Atu2575 0.24 +0.4 2,546,035 - Atu2575 0.24 +0.2 2,546,042 + Atu2575 0.24 -0.2 2,546,072 + Atu2575 0.25 -0.5 2,546,106 - Atu2575 0.27 -0.4 2,546,237 + Atu2575 0.32 -0.1 2,546,245 - Atu2575 0.32 -0.0 2,546,250 - Atu2575 0.32 +0.2 2,546,300 + Atu2575 0.35 -0.6 2,546,308 - Atu2575 0.35 +0.4 2,546,308 - Atu2575 0.35 -0.1 2,546,353 - Atu2575 0.37 -0.5 2,546,353 - Atu2575 0.37 -0.2 2,546,386 - Atu2575 0.38 +0.4 2,546,386 - Atu2575 0.38 +0.1 2,546,726 + Atu2575 0.52 +0.4 2,546,836 + Atu2575 0.56 +0.0 2,546,969 + Atu2575 0.62 +0.1 2,546,977 - Atu2575 0.62 +0.4 2,547,083 + Atu2575 0.66 +0.6 2,547,143 + Atu2575 0.69 -0.1 2,547,295 - Atu2575 0.75 +0.1 2,547,330 + Atu2575 0.76 +1.0 2,547,343 - Atu2575 0.77 +0.4 2,547,387 - Atu2575 0.79 +2.0 2,547,446 - Atu2575 0.81 +0.4 2,547,477 - Atu2575 0.82 -0.4 2,547,500 - Atu2575 0.83 -0.0 2,547,539 + Atu2575 0.85 +0.0 2,547,547 - Atu2575 0.85 -1.7 2,547,547 - Atu2575 0.85 +1.1 2,547,736 - +0.0 2,547,747 + +0.4 2,547,755 - -0.1 2,547,772 - -0.3 2,547,772 - +0.5 2,547,807 - -0.0 2,547,968 + +0.0 2,548,083 - Atu2576 0.11 -0.2 2,548,140 + Atu2576 0.14 -0.9 2,548,188 - Atu2576 0.17 -0.1 2,548,312 + Atu2576 0.23 +0.5 2,548,504 + Atu2576 0.34 +0.1 2,548,596 + Atu2576 0.39 +0.5 2,548,662 + Atu2576 0.42 -0.0 2,548,717 + Atu2576 0.45 +0.1 2,548,752 + Atu2576 0.47 -0.4 2,548,775 + Atu2576 0.48 -0.2 2,548,843 + Atu2576 0.52 -0.0 2,548,843 + Atu2576 0.52 +0.1 2,548,851 - Atu2576 0.52 -0.5 2,548,861 - Atu2576 0.53 +0.1
Or see this region's nucleotide sequence