Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2126 and Atu2127 are separated by 76 nucleotides Atu2127 and Atu2128 are separated by 8 nucleotides
Atu2126: Atu2126 - hypothetical protein, at 2,091,272 to 2,092,249
Atu2126
Atu2127: Atu2127 - L-carnitine dehydratase, at 2,092,326 to 2,093,528
Atu2127
Atu2128: Atu2128 - acetolactate synthase large subunit, at 2,093,537 to 2,095,189
Atu2128
Position (kb)
2092
2093
2094 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2092.295 kb on + strand at 2092.320 kb on + strand at 2092.442 kb on + strand at 2092.488 kb on + strand, within Atu2127 at 2092.598 kb on - strand, within Atu2127 at 2092.604 kb on - strand, within Atu2127 at 2092.637 kb on - strand, within Atu2127 at 2092.683 kb on + strand, within Atu2127 at 2092.693 kb on + strand, within Atu2127 at 2092.701 kb on - strand, within Atu2127 at 2092.756 kb on - strand, within Atu2127 at 2092.778 kb on + strand, within Atu2127 at 2092.778 kb on + strand, within Atu2127 at 2092.913 kb on + strand, within Atu2127 at 2092.938 kb on + strand, within Atu2127 at 2092.976 kb on - strand, within Atu2127 at 2093.036 kb on + strand, within Atu2127 at 2093.104 kb on - strand, within Atu2127 at 2093.296 kb on + strand, within Atu2127 at 2093.738 kb on - strand, within Atu2128 at 2093.781 kb on - strand, within Atu2128 at 2093.934 kb on - strand, within Atu2128 at 2094.037 kb on + strand, within Atu2128 at 2094.045 kb on - strand, within Atu2128 at 2094.058 kb on + strand, within Atu2128 at 2094.163 kb on - strand, within Atu2128 at 2094.268 kb on + strand, within Atu2128 at 2094.380 kb on + strand, within Atu2128 at 2094.400 kb on + strand, within Atu2128 at 2094.454 kb on - strand, within Atu2128 at 2094.491 kb on - strand, within Atu2128
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,092,295 + +0.5 2,092,320 + -0.1 2,092,442 + -1.6 2,092,488 + Atu2127 0.13 -1.4 2,092,598 - Atu2127 0.23 +0.6 2,092,604 - Atu2127 0.23 +0.0 2,092,637 - Atu2127 0.26 -1.1 2,092,683 + Atu2127 0.30 -0.8 2,092,693 + Atu2127 0.31 -0.1 2,092,701 - Atu2127 0.31 +0.6 2,092,756 - Atu2127 0.36 +1.1 2,092,778 + Atu2127 0.38 -1.1 2,092,778 + Atu2127 0.38 -2.2 2,092,913 + Atu2127 0.49 -2.1 2,092,938 + Atu2127 0.51 -0.3 2,092,976 - Atu2127 0.54 -0.4 2,093,036 + Atu2127 0.59 -1.2 2,093,104 - Atu2127 0.65 -1.2 2,093,296 + Atu2127 0.81 -0.3 2,093,738 - Atu2128 0.12 -1.2 2,093,781 - Atu2128 0.15 +0.1 2,093,934 - Atu2128 0.24 -0.0 2,094,037 + Atu2128 0.30 -0.2 2,094,045 - Atu2128 0.31 +0.5 2,094,058 + Atu2128 0.32 +0.3 2,094,163 - Atu2128 0.38 +0.1 2,094,268 + Atu2128 0.44 +1.9 2,094,380 + Atu2128 0.51 -1.0 2,094,400 + Atu2128 0.52 +0.2 2,094,454 - Atu2128 0.55 -0.8 2,094,491 - Atu2128 0.58 -2.2
Or see this region's nucleotide sequence