Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0672 and Atu0673 are separated by 25 nucleotides Atu0673 and Atu0674 are separated by 6 nucleotides Atu0674 and Atu0675 overlap by 17 nucleotides Atu0675 and Atu0676 are separated by 156 nucleotides
Atu0672: Atu0672 - transcriptional regulator, LysR family, at 668,543 to 669,460
Atu0672
Atu0673: Atu0673 - hypothetical protein, at 669,486 to 670,250
Atu0673
Atu0674: Atu0674 - hypothetical protein, at 670,257 to 670,526
Atu0674
Atu0675: Atu0675 - hypothetical protein, at 670,510 to 670,737
Atu0675
Atu0676: Atu0676 - histidyl-tRNA synthetase, at 670,894 to 672,417
Atu0676
Position (kb) 670
671 Strain fitness (log2 ratio) -1
0
1 at 669.382 kb on - strand at 669.475 kb on - strand at 669.628 kb on + strand, within Atu0673 at 669.628 kb on + strand, within Atu0673 at 669.655 kb on - strand, within Atu0673 at 669.693 kb on + strand, within Atu0673 at 669.762 kb on - strand, within Atu0673 at 669.784 kb on - strand, within Atu0673 at 670.003 kb on + strand, within Atu0673 at 670.038 kb on - strand, within Atu0673 at 670.038 kb on - strand, within Atu0673 at 670.058 kb on - strand, within Atu0673 at 670.134 kb on + strand, within Atu0673 at 670.159 kb on + strand, within Atu0673 at 670.245 kb on - strand at 670.245 kb on - strand at 670.245 kb on - strand at 670.285 kb on + strand, within Atu0674 at 670.285 kb on + strand, within Atu0674 at 670.319 kb on + strand, within Atu0674 at 670.383 kb on + strand, within Atu0674 at 670.498 kb on + strand, within Atu0674 at 670.516 kb on + strand at 670.524 kb on - strand at 670.529 kb on - strand at 670.534 kb on - strand, within Atu0675 at 670.841 kb on + strand at 670.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 669,382 - +0.5 669,475 - +0.3 669,628 + Atu0673 0.19 +0.2 669,628 + Atu0673 0.19 +1.5 669,655 - Atu0673 0.22 -0.2 669,693 + Atu0673 0.27 +0.1 669,762 - Atu0673 0.36 +1.3 669,784 - Atu0673 0.39 +0.4 670,003 + Atu0673 0.68 +0.8 670,038 - Atu0673 0.72 -0.2 670,038 - Atu0673 0.72 +0.1 670,058 - Atu0673 0.75 -1.2 670,134 + Atu0673 0.85 +0.6 670,159 + Atu0673 0.88 -0.3 670,245 - +0.4 670,245 - -0.1 670,245 - +0.3 670,285 + Atu0674 0.10 +0.4 670,285 + Atu0674 0.10 -0.2 670,319 + Atu0674 0.23 +0.5 670,383 + Atu0674 0.47 -0.5 670,498 + Atu0674 0.89 +0.0 670,516 + -0.9 670,524 - -0.1 670,529 - -0.3 670,534 - Atu0675 0.11 +1.4 670,841 + +0.1 670,849 + +0.4
Or see this region's nucleotide sequence