Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0474 and Atu0475 overlap by 4 nucleotides Atu0475 and Atu0476 are separated by 268 nucleotides Atu0476 and Atu0477 are separated by 22 nucleotides Atu0477 and Atu0478 are separated by 211 nucleotides
Atu0474: Atu0474 - putative RNA polymerase sigma-70 factor, at 462,837 to 463,382
Atu0474
Atu0475: Atu0475 - hypothetical protein, at 463,379 to 464,083
Atu0475
Atu0476: Atu0476 - aquaporin, at 464,352 to 465,077
Atu0476
Atu0477: Atu0477 - hypothetical protein, at 465,100 to 465,423
Atu0477
Atu0478: Atu0478 - glycosyltransferase, at 465,635 to 467,182
Atu0478
Position (kb)
464
465
466 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 463.419 kb on + strand at 463.424 kb on + strand at 463.424 kb on + strand at 463.444 kb on + strand at 463.452 kb on - strand, within Atu0475 at 463.475 kb on + strand, within Atu0475 at 463.475 kb on + strand, within Atu0475 at 463.539 kb on + strand, within Atu0475 at 463.564 kb on - strand, within Atu0475 at 463.564 kb on - strand, within Atu0475 at 463.564 kb on - strand, within Atu0475 at 463.662 kb on + strand, within Atu0475 at 463.715 kb on + strand, within Atu0475 at 463.745 kb on - strand, within Atu0475 at 463.834 kb on - strand, within Atu0475 at 463.837 kb on - strand, within Atu0475 at 463.861 kb on + strand, within Atu0475 at 463.861 kb on + strand, within Atu0475 at 463.867 kb on + strand, within Atu0475 at 463.869 kb on - strand, within Atu0475 at 463.869 kb on - strand, within Atu0475 at 463.876 kb on + strand, within Atu0475 at 463.876 kb on + strand, within Atu0475 at 463.876 kb on + strand, within Atu0475 at 463.879 kb on - strand, within Atu0475 at 463.884 kb on - strand, within Atu0475 at 463.884 kb on - strand, within Atu0475 at 463.884 kb on - strand, within Atu0475 at 463.932 kb on + strand, within Atu0475 at 463.987 kb on + strand, within Atu0475 at 464.009 kb on - strand, within Atu0475 at 464.009 kb on - strand, within Atu0475 at 464.127 kb on - strand at 464.220 kb on + strand at 464.228 kb on - strand at 464.228 kb on - strand at 464.347 kb on + strand at 464.414 kb on - strand at 464.414 kb on - strand at 464.477 kb on - strand, within Atu0476 at 464.489 kb on + strand, within Atu0476 at 464.495 kb on + strand, within Atu0476 at 464.539 kb on + strand, within Atu0476 at 464.623 kb on - strand, within Atu0476 at 464.658 kb on - strand, within Atu0476 at 464.702 kb on + strand, within Atu0476 at 464.795 kb on - strand, within Atu0476 at 464.845 kb on + strand, within Atu0476 at 464.845 kb on + strand, within Atu0476 at 464.845 kb on + strand, within Atu0476 at 464.853 kb on - strand, within Atu0476 at 464.853 kb on - strand, within Atu0476 at 464.853 kb on - strand, within Atu0476 at 464.853 kb on - strand, within Atu0476 at 464.853 kb on - strand, within Atu0476 at 464.857 kb on + strand, within Atu0476 at 464.857 kb on + strand, within Atu0476 at 464.865 kb on - strand, within Atu0476 at 464.928 kb on - strand, within Atu0476 at 464.932 kb on - strand, within Atu0476 at 464.941 kb on + strand, within Atu0476 at 464.957 kb on + strand, within Atu0476 at 464.957 kb on + strand, within Atu0476 at 464.960 kb on - strand, within Atu0476 at 464.962 kb on + strand, within Atu0476 at 464.974 kb on - strand, within Atu0476 at 465.172 kb on + strand, within Atu0477 at 465.384 kb on - strand, within Atu0477 at 465.410 kb on - strand at 465.448 kb on + strand at 465.474 kb on - strand at 465.538 kb on + strand at 465.538 kb on + strand at 465.600 kb on + strand at 465.609 kb on + strand at 465.610 kb on - strand at 465.709 kb on - strand at 465.711 kb on - strand at 465.760 kb on + strand at 465.882 kb on + strand, within Atu0478 at 465.922 kb on - strand, within Atu0478 at 465.952 kb on - strand, within Atu0478 at 465.964 kb on - strand, within Atu0478 at 465.998 kb on + strand, within Atu0478 at 466.042 kb on + strand, within Atu0478 at 466.050 kb on - strand, within Atu0478
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 463,419 + +0.1 463,424 + -1.7 463,424 + -0.5 463,444 + -1.2 463,452 - Atu0475 0.10 +0.4 463,475 + Atu0475 0.14 +0.5 463,475 + Atu0475 0.14 -1.8 463,539 + Atu0475 0.23 +0.3 463,564 - Atu0475 0.26 +0.8 463,564 - Atu0475 0.26 -0.4 463,564 - Atu0475 0.26 -0.3 463,662 + Atu0475 0.40 -0.1 463,715 + Atu0475 0.48 +0.2 463,745 - Atu0475 0.52 -0.2 463,834 - Atu0475 0.65 -1.4 463,837 - Atu0475 0.65 -0.6 463,861 + Atu0475 0.68 +0.3 463,861 + Atu0475 0.68 +0.1 463,867 + Atu0475 0.69 +0.0 463,869 - Atu0475 0.70 -1.0 463,869 - Atu0475 0.70 +0.4 463,876 + Atu0475 0.70 +0.2 463,876 + Atu0475 0.70 +0.1 463,876 + Atu0475 0.70 +0.1 463,879 - Atu0475 0.71 +2.6 463,884 - Atu0475 0.72 +0.5 463,884 - Atu0475 0.72 -0.3 463,884 - Atu0475 0.72 -0.9 463,932 + Atu0475 0.78 +0.6 463,987 + Atu0475 0.86 -0.1 464,009 - Atu0475 0.89 -0.5 464,009 - Atu0475 0.89 -0.5 464,127 - +0.2 464,220 + -1.1 464,228 - -0.5 464,228 - +0.2 464,347 + +0.4 464,414 - -1.0 464,414 - +1.4 464,477 - Atu0476 0.17 -0.5 464,489 + Atu0476 0.19 +0.4 464,495 + Atu0476 0.20 -0.3 464,539 + Atu0476 0.26 +0.9 464,623 - Atu0476 0.37 -0.4 464,658 - Atu0476 0.42 -1.3 464,702 + Atu0476 0.48 -0.1 464,795 - Atu0476 0.61 -0.4 464,845 + Atu0476 0.68 +0.1 464,845 + Atu0476 0.68 -0.1 464,845 + Atu0476 0.68 -1.1 464,853 - Atu0476 0.69 +0.8 464,853 - Atu0476 0.69 -0.2 464,853 - Atu0476 0.69 +0.3 464,853 - Atu0476 0.69 -0.3 464,853 - Atu0476 0.69 -0.4 464,857 + Atu0476 0.70 +0.1 464,857 + Atu0476 0.70 -0.1 464,865 - Atu0476 0.71 -0.1 464,928 - Atu0476 0.79 +0.1 464,932 - Atu0476 0.80 -0.2 464,941 + Atu0476 0.81 -1.3 464,957 + Atu0476 0.83 -0.3 464,957 + Atu0476 0.83 -0.9 464,960 - Atu0476 0.84 -0.3 464,962 + Atu0476 0.84 -0.6 464,974 - Atu0476 0.86 -1.3 465,172 + Atu0477 0.22 +0.1 465,384 - Atu0477 0.88 -1.0 465,410 - +0.2 465,448 + -0.4 465,474 - -1.5 465,538 + -0.2 465,538 + -0.1 465,600 + -0.4 465,609 + -0.1 465,610 - +1.1 465,709 - +1.4 465,711 - -0.3 465,760 + -0.5 465,882 + Atu0478 0.16 +0.1 465,922 - Atu0478 0.19 +0.8 465,952 - Atu0478 0.20 +1.0 465,964 - Atu0478 0.21 -0.4 465,998 + Atu0478 0.23 -0.2 466,042 + Atu0478 0.26 +0.3 466,050 - Atu0478 0.27 +0.1
Or see this region's nucleotide sequence