Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0188 and Atu0189 overlap by 1 nucleotides Atu0189 and Atu0190 are separated by 5 nucleotides
Atu0188: Atu0188 - ABC transporter, membrane spanning protein (peptide), at 191,514 to 192,608
Atu0188
Atu0189: Atu0189 - ABC transporter, membrane spanning protein (peptide), at 192,608 to 193,753
Atu0189
Atu0190: Atu0190 - ABC transporter, nucleotide binding/ATPase protein (peptide), at 193,759 to 195,399
Atu0190
Position (kb) 192
193
194 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 191.669 kb on + strand, within Atu0188 at 191.760 kb on - strand, within Atu0188 at 191.937 kb on - strand, within Atu0188 at 192.427 kb on - strand, within Atu0188 at 192.507 kb on + strand at 192.633 kb on + strand at 192.649 kb on + strand at 192.685 kb on - strand at 192.716 kb on + strand at 192.959 kb on - strand, within Atu0189 at 193.621 kb on + strand, within Atu0189 at 193.635 kb on - strand, within Atu0189 at 193.647 kb on + strand at 193.647 kb on + strand at 193.655 kb on - strand at 193.725 kb on + strand at 193.735 kb on + strand at 193.825 kb on + strand at 193.864 kb on - strand at 194.003 kb on + strand, within Atu0190 at 194.025 kb on + strand, within Atu0190 at 194.362 kb on - strand, within Atu0190 at 194.388 kb on + strand, within Atu0190 at 194.466 kb on + strand, within Atu0190 at 194.497 kb on - strand, within Atu0190 at 194.507 kb on - strand, within Atu0190 at 194.520 kb on + strand, within Atu0190 at 194.529 kb on - strand, within Atu0190 at 194.687 kb on + strand, within Atu0190 at 194.704 kb on + strand, within Atu0190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 191,669 + Atu0188 0.14 -2.0 191,760 - Atu0188 0.22 +1.4 191,937 - Atu0188 0.39 -0.6 192,427 - Atu0188 0.83 -1.5 192,507 + -2.0 192,633 + -1.0 192,649 + -0.9 192,685 - -1.5 192,716 + -0.4 192,959 - Atu0189 0.31 -1.1 193,621 + Atu0189 0.88 -2.2 193,635 - Atu0189 0.90 -0.7 193,647 + +0.6 193,647 + -3.0 193,655 - -0.7 193,725 + -1.2 193,735 + -1.5 193,825 + -0.6 193,864 - -1.6 194,003 + Atu0190 0.15 -1.8 194,025 + Atu0190 0.16 -3.6 194,362 - Atu0190 0.37 -1.5 194,388 + Atu0190 0.38 -2.0 194,466 + Atu0190 0.43 -1.6 194,497 - Atu0190 0.45 -1.4 194,507 - Atu0190 0.46 -0.1 194,520 + Atu0190 0.46 -2.1 194,529 - Atu0190 0.47 +0.0 194,687 + Atu0190 0.57 -1.4 194,704 + Atu0190 0.58 -3.0
Or see this region's nucleotide sequence