Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0187 and Atu0188 are separated by 141 nucleotides Atu0188 and Atu0189 overlap by 1 nucleotides
Atu0187: Atu0187 - ABC transporter, substrate binding protein (peptide), at 189,528 to 191,372
Atu0187
Atu0188: Atu0188 - ABC transporter, membrane spanning protein (peptide), at 191,514 to 192,608
Atu0188
Atu0189: Atu0189 - ABC transporter, membrane spanning protein (peptide), at 192,608 to 193,753
Atu0189
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1 at 190.571 kb on - strand, within Atu0187 at 190.600 kb on - strand, within Atu0187 at 190.743 kb on + strand, within Atu0187 at 190.743 kb on + strand, within Atu0187 at 190.878 kb on + strand, within Atu0187 at 190.918 kb on + strand, within Atu0187 at 190.977 kb on + strand, within Atu0187 at 190.992 kb on - strand, within Atu0187 at 191.001 kb on - strand, within Atu0187 at 191.044 kb on - strand, within Atu0187 at 191.237 kb on + strand at 191.237 kb on + strand at 191.245 kb on - strand at 191.245 kb on - strand at 191.301 kb on - strand at 191.361 kb on + strand at 191.505 kb on - strand at 191.544 kb on - strand at 191.669 kb on + strand, within Atu0188 at 191.760 kb on - strand, within Atu0188 at 191.937 kb on - strand, within Atu0188 at 192.427 kb on - strand, within Atu0188 at 192.507 kb on + strand at 192.633 kb on + strand at 192.649 kb on + strand at 192.685 kb on - strand at 192.716 kb on + strand at 192.959 kb on - strand, within Atu0189
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 190,571 - Atu0187 0.57 +0.2 190,600 - Atu0187 0.58 +1.4 190,743 + Atu0187 0.66 +0.1 190,743 + Atu0187 0.66 +0.4 190,878 + Atu0187 0.73 -1.2 190,918 + Atu0187 0.75 +0.0 190,977 + Atu0187 0.79 +0.9 190,992 - Atu0187 0.79 -0.4 191,001 - Atu0187 0.80 +0.3 191,044 - Atu0187 0.82 +0.0 191,237 + +0.7 191,237 + -0.0 191,245 - +1.4 191,245 - -0.6 191,301 - -0.5 191,361 + +0.2 191,505 - -1.6 191,544 - -1.0 191,669 + Atu0188 0.14 -2.0 191,760 - Atu0188 0.22 +1.4 191,937 - Atu0188 0.39 -0.6 192,427 - Atu0188 0.83 -1.5 192,507 + -2.0 192,633 + -1.0 192,649 + -0.9 192,685 - -1.5 192,716 + -0.4 192,959 - Atu0189 0.31 -1.1
Or see this region's nucleotide sequence