Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0037 and Atu0038 are separated by 29 nucleotides Atu0038 and Atu0039 are separated by 15 nucleotides Atu0039 and Atu0040 overlap by 4 nucleotides
Atu0037: Atu0037 - pantothenate kinase, at 39,781 to 40,749
Atu0037
Atu0038: Atu0038 - phosphoribosyl-ATP pyrophosphohydrolase, at 40,779 to 41,102
Atu0038
Atu0039: Atu0039 - imidazoleglycerol-phosphate synthase cyclase, at 41,118 to 41,894
Atu0039
Atu0040: Atu0040 - phosphoribosylformino-5-aminoimidazole carboxamide ribotide isomerase, at 41,891 to 42,634
Atu0040
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1
2 at 40.759 kb on - strand at 40.815 kb on + strand, within Atu0038 at 40.818 kb on - strand, within Atu0038 at 40.820 kb on + strand, within Atu0038 at 40.823 kb on - strand, within Atu0038 at 40.823 kb on - strand, within Atu0038 at 40.823 kb on - strand, within Atu0038 at 41.108 kb on - strand at 41.142 kb on + strand at 41.158 kb on + strand at 41.239 kb on + strand, within Atu0039 at 41.565 kb on - strand, within Atu0039 at 41.573 kb on - strand, within Atu0039 at 41.671 kb on + strand, within Atu0039 at 41.834 kb on - strand at 41.905 kb on + strand at 41.913 kb on + strand at 42.070 kb on - strand, within Atu0040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 40,759 - -1.3 40,815 + Atu0038 0.11 -1.0 40,818 - Atu0038 0.12 +0.3 40,820 + Atu0038 0.13 -1.6 40,823 - Atu0038 0.14 -0.3 40,823 - Atu0038 0.14 +0.2 40,823 - Atu0038 0.14 +0.3 41,108 - +1.2 41,142 + +0.3 41,158 + +2.2 41,239 + Atu0039 0.16 +1.2 41,565 - Atu0039 0.58 +1.2 41,573 - Atu0039 0.59 -2.4 41,671 + Atu0039 0.71 -1.3 41,834 - -0.3 41,905 + -0.2 41,913 + -1.1 42,070 - Atu0040 0.24 +0.9
Or see this region's nucleotide sequence