Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4760 and GFF4761 overlap by 4 nucleotides GFF4761 and GFF4762 are separated by 23 nucleotides GFF4762 and GFF4763 are separated by 24 nucleotides
GFF4760 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 66,460 to 67,755
GFF4760
GFF4761 - Type III secretion system protein BsaR, at 67,752 to 68,159
GFF4761
GFF4762 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 68,183 to 70,240
GFF4762
GFF4763 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 70,265 to 71,383
GFF4763
Position (kb)
68
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1 at 67.319 kb on - strand, within GFF4760 at 67.380 kb on - strand, within GFF4760 at 67.527 kb on - strand, within GFF4760 at 67.594 kb on + strand, within GFF4760 at 67.595 kb on - strand, within GFF4760 at 67.696 kb on - strand at 67.730 kb on + strand at 67.731 kb on - strand at 67.860 kb on - strand, within GFF4761 at 67.962 kb on + strand, within GFF4761 at 68.005 kb on - strand, within GFF4761 at 68.160 kb on - strand at 68.305 kb on + strand at 68.336 kb on + strand at 68.350 kb on + strand at 68.533 kb on + strand, within GFF4762 at 68.656 kb on + strand, within GFF4762 at 69.089 kb on - strand, within GFF4762 at 69.150 kb on - strand, within GFF4762 at 69.391 kb on + strand, within GFF4762 at 69.449 kb on - strand, within GFF4762 at 69.491 kb on - strand, within GFF4762 at 69.550 kb on + strand, within GFF4762 at 69.551 kb on - strand, within GFF4762 at 69.551 kb on - strand, within GFF4762 at 69.553 kb on - strand, within GFF4762 at 69.752 kb on - strand, within GFF4762 at 69.752 kb on - strand, within GFF4762 at 69.800 kb on - strand, within GFF4762 at 69.883 kb on + strand, within GFF4762 at 70.071 kb on + strand at 70.209 kb on - strand at 70.334 kb on - strand at 70.334 kb on - strand at 70.530 kb on - strand, within GFF4763 at 70.601 kb on + strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.829 kb on + strand, within GFF4763 at 71.009 kb on + strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.076 kb on - strand, within GFF4763 at 71.084 kb on + strand, within GFF4763
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 67,319 - GFF4760 0.66 +1.3 67,380 - GFF4760 0.71 +0.2 67,527 - GFF4760 0.82 -0.1 67,594 + GFF4760 0.88 +0.2 67,595 - GFF4760 0.88 -0.9 67,696 - -0.2 67,730 + -0.3 67,731 - +0.1 67,860 - GFF4761 0.26 -0.2 67,962 + GFF4761 0.51 +1.3 68,005 - GFF4761 0.62 +0.3 68,160 - +0.2 68,305 + -0.8 68,336 + -0.6 68,350 + +0.1 68,533 + GFF4762 0.17 -0.2 68,656 + GFF4762 0.23 -0.4 69,089 - GFF4762 0.44 -0.1 69,150 - GFF4762 0.47 +0.7 69,391 + GFF4762 0.59 -0.9 69,449 - GFF4762 0.62 +0.1 69,491 - GFF4762 0.64 +0.1 69,550 + GFF4762 0.66 +0.1 69,551 - GFF4762 0.66 -0.4 69,551 - GFF4762 0.66 +0.2 69,553 - GFF4762 0.67 +0.6 69,752 - GFF4762 0.76 -1.1 69,752 - GFF4762 0.76 -0.0 69,800 - GFF4762 0.79 +0.1 69,883 + GFF4762 0.83 +0.3 70,071 + -0.2 70,209 - -1.9 70,334 - -0.3 70,334 - -0.3 70,530 - GFF4763 0.24 +0.2 70,601 + GFF4763 0.30 +0.2 70,753 - GFF4763 0.44 -0.4 70,753 - GFF4763 0.44 -0.3 70,753 - GFF4763 0.44 -0.6 70,829 + GFF4763 0.50 -0.2 71,009 + GFF4763 0.66 +0.2 71,010 - GFF4763 0.67 -0.9 71,010 - GFF4763 0.67 +0.4 71,076 - GFF4763 0.72 +0.3 71,084 + GFF4763 0.73 -0.2
Or see this region's nucleotide sequence